MCF 10A-H2B-mCherry - Cell Line (ID:51124)
| HMS LINCS ID: | 51124 |
| Name: | MCF 10A-H2B-mCherry |
| Alternative Names: | |
| LINCS ID: | |
| Alternative ID: | CLO_0007599 |
| Parent Cell Line: | HMSL50583-7: MCF 10A |
| Reference Source: | Mario Niepel (Harvard Medical School) |
| Organism: | Homo sapiens |
| Organ: | breast |
| Tissue: | |
| Cell Type: | epithelial |
| Details of Cell Type: | |
| Donor Sex: | female |
| Donor Age: | |
| Donor Ethnicity: | |
| Donor Health Status: | |
| Disease: | fibrocystic disease |
| Details of Disease: | non-tumorigenic |
| Production Details: | Modification type: retroviral transformation with an H2B-mCherry expression cassette that comprised AAVS1 homology arms, the hPGK promotor, and SV40 polyA terminator and integration into the AAVS1 safe harbor genomic locus using CRISPR/Cas9; followed by clonal selection based on transgene expression level. Citations: H2B-mCherry expression cassette: gift of Robert Benezra (Memorial Sloan Kettering Cancer Center), Addgene plasmid #20972; SV40 polyA terminator: gift from Rudolf Jaenisch (Whitehead Institute for Biomedical Research), Addgene plasmid # 22072. |
| Genetic Modification(s): | H2B-mCherry integration at AAVS1 safe harbor locus |
| Known Mutations: | |
| Citation Information for Mutations: | |
| Verification Reference Profile: | |
| Growth Properties: | adherent |
| Recommended Culture Conditions: | |
| Relevant Citations: | PMID 27135972 |
| Usage Note: | |
| Comments: | |
| Date Publicly Available: | 2016-08-24 |
| Most Recent Update: | 2016-08-24 |
Datasets:
| HMS Dataset ID | Dataset Title | HMS Dataset Type |
|---|---|---|
| 20278 | LINCS MCF 10A Common Project: Fixed-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 1 of 9: Cell count and normalized growth rate inhibition values for biological replicate 1 | Microscopy/Imaging |
| 20279 | LINCS MCF 10A Common Project: Fixed-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 2 of 9: Cell count and normalized growth rate inhibition values for biological replicate 2 | Microscopy/Imaging |
| 20280 | LINCS MCF 10A Common Project: Fixed-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 3 of 9: Cell count and normalized growth rate inhibition values for biological replicate 3 | Microscopy/Imaging |
| 20281 | LINCS MCF 10A Common Project: Fixed-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 4 of 9: Mean cell count and mean normalized growth rate inhibition values for biological replicate 1 | Microscopy/Imaging |
| 20282 | LINCS MCF 10A Common Project: Fixed-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 5 of 9: Mean cell count and mean normalized growth rate inhibition values for biological replicate 2 | Microscopy/Imaging |
| 20283 | LINCS MCF 10A Common Project: Fixed-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 6 of 9: Mean cell count and mean normalized growth rate inhibition values for biological replicate 3 | Microscopy/Imaging |
| 20284 | LINCS MCF 10A Common Project: Fixed-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 7 of 9: Calculated dose-response metrics for biological replicate 1 | Analysis |
| 20285 | LINCS MCF 10A Common Project: Fixed-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 8 of 9: Calculated dose-response metrics for biological replicate 2 | Analysis |
| 20286 | LINCS MCF 10A Common Project: Fixed-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 9 of 9: Calculated dose-response metrics for biological replicate 3 | Analysis |
| 20309 | LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 1 of 15: End-point cell counts and normalized growth rate inhibition values for all technical replicates of biological replicate 1. | Microscopy/Imaging |
| 20310 | LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 2 of 15: End-point cell counts and normalized growth rate inhibition values for all technical replicates of biological replicate 2. | Microscopy/Imaging |
| 20311 | LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 3 of 15: End-point cell counts and normalized growth rate inhibition values for all technical replicates of biological replicate 3. | Microscopy/Imaging |
| 20312 | LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 4 of 15: End-point mean cell counts and mean normalized growth rate inhibition values for biological replicate 1. | Microscopy/Imaging |
| 20313 | LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 5 of 15: End-point mean cell counts and mean normalized growth rate inhibition values for biological replicate 2. | Microscopy/Imaging |
| 20314 | LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 6 of 15: End-point mean cell counts and mean normalized growth rate inhibition values for biological replicate 3. | Microscopy/Imaging |
| 20315 | LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 7 of 15: End-point dose-response metrics for biological replicate 1. | Analysis |
| 20316 | LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 8 of 15: End-point dose-response metrics for biological replicate 2. | Analysis |
| 20317 | LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 9 of 15: End-point dose-response metrics for biological replicate 3. | Analysis |
| 20318 | LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 10 of 15: Time-dependent mean normalized growth rate inhibition values for biological replicate 1. | Microscopy/Imaging |
| 20319 | LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 11 of 15: Time-dependent mean normalized growth rate inhibition values for biological replicate 2. | Microscopy/Imaging |
| 20320 | LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 12 of 15: Time-dependent mean normalized growth rate inhibition values for biological replicate 3. | Microscopy/Imaging |
| 20321 | LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 13 of 15: Time-dependent dose-response metrics for biological replicate 1. | Analysis |
| 20322 | LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 14 of 15: Time-dependent dose-response metrics for biological replicate 2. | Analysis |
| 20323 | LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 15 of 15: Time-dependent dose-response metrics for biological replicate 3. | Analysis |
Batch Information:
| HMS LINCS Batch ID | Provider | Provider Catalog ID | Provider Batch ID |
|---|---|---|---|
| 51124-1 | Mario Niepel (Harvard Medical School) |