RVH-421 - Cell Line (ID:50475)
| HMS LINCS ID: | 50475 |
| Name: | RVH-421 |
| Alternative Names: | |
| LINCS ID: | LCL-1255 |
| Alternative ID: | CLO_0008902 |
| Parent Cell Line: | |
| Reference Source: | Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 127 |
| Organism: | Homo sapiens |
| Organ: | skin |
| Tissue: | |
| Cell Type: | |
| Details of Cell Type: | |
| Donor Sex: | male |
| Donor Age: | |
| Donor Ethnicity: | |
| Donor Health Status: | |
| Disease: | DOID:1909, malignant melanoma |
| Details of Disease: | |
| Production Details: | |
| Genetic Modification(s): | none |
| Known Mutations: | |
| Citation Information for Mutations: | COSS909706 |
| Verification Reference Profile: | |
| Growth Properties: | adherent |
| Recommended Culture Conditions: | From MGH/CMT as specified by cell provider: 90% RPMI 1640 (or Leibovitz's L-15) + 10% FBS split confluent culture up to 1:10 one to two times per week using trypsin/EDTA; seed out 1-2 x 106 cells/175 cm 2 at 37C with 5% CO2 about 15 x 106 cells/175 cm 2 |
| Relevant Citations: | |
| Usage Note: | |
| Comments: | |
| Date Publicly Available: | 2012-07-31 |
| Most Recent Update: | 2016-08-24 |
Datasets:
| HMS Dataset ID | Dataset Title | HMS Dataset Type |
|---|---|---|
| 20217 | Viability and apoptosis in BRAF(V600E/D) melanoma cell lines monitored by imaging | Microscopy/Imaging |
| 20218 | Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) | Reverse Phase Protein Array |
| 20229 | PLSR model loadings (pMEK and pERK included) from analysis of the covariation of molecular signals with cell viability and apoptosis fraction in BRAF(V600E/D) melanoma cell lines | Analysis |
| 20230 | PLSR model loadings (pMEK and pERK excluded) from analysis of the covariation of molecular signals with cell viability and apoptosis fraction in BRAF(V600E/D) melanoma cell lines | Analysis |
| 20231 | Average variable importance in the projection (VIP) scores from the PLSR models analyzing the covariation of molecular signals with cell viability and apoptosis fraction in BRAF(V600E/D) melanoma cell lines | Analysis |
Batch Information:
| HMS LINCS Batch ID | Provider | Provider Catalog ID | Provider Batch ID |
|---|---|---|---|
| 50475-1 | Nathan Moerke (Harvard Medical School) | ||
| 50475-2 | Nathan Moerke (Harvard Medical School) |