MDA-MB-436 - Cell Line (ID:50333)
| HMS LINCS ID: | 50333 |
| Name: | MDA-MB-436 |
| Alternative Names: | |
| LINCS ID: | LCL-1470 |
| Alternative ID: | CLO_0007639 |
| Parent Cell Line: | |
| Reference Source: | ATCC HTB-130 |
| Organism: | Homo sapiens |
| Organ: | breast |
| Tissue: | |
| Cell Type: | |
| Details of Cell Type: | |
| Donor Sex: | female |
| Donor Age: | |
| Donor Ethnicity: | |
| Donor Health Status: | |
| Disease: | DOID:3458, adenocarcinoma |
| Details of Disease: | breast adenocarcinoma |
| Production Details: | |
| Genetic Modification(s): | none |
| Known Mutations: | |
| Citation Information for Mutations: | COSS1240172 |
| Verification Reference Profile: | |
| Growth Properties: | adherent |
| Recommended Culture Conditions: | From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 10 mcg/ml insulin, 16 mcg/ml glutathione, 90%; fetal bovine serum, 10%. Subculturing: Subcultures are prepared by scraping. Remove spent medium, add 3 to 5 ml of fresh medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subculture every 6 to 8 days. Subcultivation ratio: A subcultivation ratio of 1:2 is recommended |
| Relevant Citations: | |
| Usage Note: | |
| Comments: | |
| Date Publicly Available: | 2012-07-31 |
| Most Recent Update: | 2016-04-04 |
Batch Information for HMSL50333-2:
| HMS LINCS Batch ID: | 50333-2 |
| Provider: | ATCC |
| Provider Catalog ID: | HTB-130 |
| Provider Batch ID: | |
| Source Information: | Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010 |
| Date Received: | 2010-07-16 |
| HMS QC Outcome: | |
| Transient Modification(s): | |
| Comments: | |
| Date Publicly Available: | 2016-06-06 |
| Most Recent Update: | 2016-07-12 |