BT-483 - Cell Line (ID:50107)

Name: BT-483
Alternative Names:
Alternative ID: CLO_0002043
Parent Cell Line:
Reference Source: ATCC HTB-121
Organism: Homo sapiens
Organ: breast
Cell Type:
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3008, ductal carcinoma
Details of Disease: breast ductal carcinoma
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS949093
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.01 mg/ml bovine insulin, 80%; fetal bovine serum, 20%. Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 3 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subcultivation ratio: A subcultivation ratio of 1:2 is recommended
Relevant Citations:
Usage Note:
Date Publicly Available: 2012-07-31
Most Recent Update: 2016-04-04

Batch Information for HMSL50107-1:

HMS LINCS Batch ID: 50107-1
Provider: ATCC
Provider Catalog ID: HTB-121
Provider Batch ID:
Source Information: Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC
Date Received:
HMS QC Outcome:
Transient Modification(s):
Date Publicly Available: 2012-07-31
Most Recent Update: 2015-06-29