SK-BR-3 - Cell Line (ID:50057)

Name: SK-BR-3
Alternative Names: SKBr3
Alternative ID: CLO_0009034
Parent Cell Line:
Reference Source: ATCC HTB-30
Organism: Homo sapiens
Organ: breast
Cell Type: epithelial
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3459, breast carcinoma
Details of Disease:
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations:
Verification Reference Profile: DNA Profile (STR, source: ATCC): Amelogenin: X CSF1PO: 12 D13S317: 11,12 D16S539: 9 D5S818: 9,12 D7S820: 9,12 THO1: 8,9 TPOX: 8,11 vWA: 17
Growth Properties: adherent
Recommended Culture Conditions: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 deg C Subculturing protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin, 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37 deg C. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: 2 to 3 times per week
Relevant Citations:
Usage Note:
Date Publicly Available: 2012-05-01
Most Recent Update: 2016-07-12

Batch Information for HMSL50057-1:

HMS LINCS Batch ID: 50057-1
Provider: ATCC
Provider Catalog ID: HTB-30
Provider Batch ID:
Source Information: Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC
Date Received:
HMS QC Outcome:
Transient Modification(s):
Date Publicly Available: 2012-05-01
Most Recent Update: 2015-06-29