| HMS LINCS ID:
|
50049
|
| Name:
|
WiDr
|
| Alternative Names:
|
|
| LINCS ID:
|
LCL-1169
|
| Alternative ID:
|
CLO_0009600
|
| Parent Cell Line:
|
|
| Reference Source:
|
ATCC CCL-218
|
| Organism:
|
Homo sapiens
|
| Organ:
|
intestine
|
| Tissue:
|
|
| Cell Type:
|
epithelial
|
| Details of Cell Type:
|
|
| Donor Sex:
|
female
|
| Donor Age:
|
|
| Donor Ethnicity:
|
|
| Donor Health Status:
|
|
| Disease:
|
DOID:1520, DOID:234, colon adenocarcinoma
|
| Details of Disease:
|
|
| Production Details:
|
|
| Genetic Modification(s):
|
none
|
| Known Mutations:
|
|
| Citation Information for Mutations:
|
|
| Verification Reference Profile:
|
STR-PCR Data (source: ECACC):
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19
|
| Growth Properties:
|
adherent
|
| Recommended Culture Conditions:
|
From MGH/CMT as specified by cell provider: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
|
| Relevant Citations:
|
|
| Usage Note:
|
|
| Comments:
|
|
| Date Publicly Available:
|
2012-04-25
|
| Most Recent Update:
|
2016-07-12
|