| HMS LINCS ID:
|
50043
|
| Name:
|
SK-MES
|
| Alternative Names:
|
SK-MES-1; SK MES 1
|
| LINCS ID:
|
LCL-1583
|
| Alternative ID:
|
CLO_0009049
|
| Parent Cell Line:
|
|
| Reference Source:
|
ATCC HTB-58
|
| Organism:
|
Homo sapiens
|
| Organ:
|
lung
|
| Tissue:
|
|
| Cell Type:
|
epithelial
|
| Details of Cell Type:
|
|
| Donor Sex:
|
male
|
| Donor Age:
|
|
| Donor Ethnicity:
|
|
| Donor Health Status:
|
|
| Disease:
|
DOID:3907, lung squamous cell carcinoma
|
| Details of Disease:
|
NSCLC squamous cell carcinoma
|
| Production Details:
|
|
| Genetic Modification(s):
|
none
|
| Known Mutations:
|
|
| Citation Information for Mutations:
|
COSS909728
|
| Verification Reference Profile:
|
STR-PCR Data (source: ECACC):
Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 13
D5S818: 11
D7S820: 8
THO1: 6,9.3
TPOX: 8
vWA: 14
|
| Growth Properties:
|
adherent
|
| Recommended Culture Conditions:
|
From MGH/CMT as specified by cell provider: Ham's F10 : DMEM (1:1) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 i.e. seeding at 4-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
|
| Relevant Citations:
|
|
| Usage Note:
|
|
| Comments:
|
|
| Date Publicly Available:
|
2012-04-25
|
| Most Recent Update:
|
2016-04-04
|