Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
LOXIMVI 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.3846 1.1933 0.2688 0.2410 0.2160 0.4386 0.3497 1.3986 0.3333 0.4098 1.0941 0.3571 0.3968 0.1587 0.1880 1.1086 0.2770
LOXIMVI 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.2994 1.3193 0.2315 0.2252 0.2169 0.4115 0.3425 1.4347 0.4505 1.1429 0.4000 0.4673 0.1155 0.2212 1.0091 0.2841
LOXIMVI 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.3759 1.2500 0.2849 0.2387 0.2114 0.4630 0.3401 1.4793 0.3367 0.4237 1.1338 0.3891 0.4184 0.1642 0.1927 0.9804 0.2725
LOXIMVI 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.2924 1.0504 0.2320 0.2331 0.1969 0.3984 0.3472 1.4006 0.2950 0.3891 1.0764 0.3759 0.4405 0.0758 1.0183 0.2604
LOXIMVI 0.0000
uM
 5
hr
 2
Odyssey/Microvigene
 0.3788 1.0776 0.2865 0.2451 0.2203 0.4032 0.3436 1.3661 0.3425 0.3861 1.1601 0.4274 0.3937 0.1435 0.2083 1.0101 0.2924
LOXIMVI 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.3058 1.3755 0.2445 0.2410 0.1961 0.4082 0.3534 1.4472 0.3378 0.4425 1.1834 0.3802 0.3968 0.0990 0.2041 1.0764 0.2710
LOXIMVI 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.3012 1.2821 0.2404 0.2433 0.2075 0.4082 0.3509 1.4124 0.3636 0.4184 1.0953 0.4032 0.1148 0.1919 1.0893 0.2865
LOXIMVI 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.3509 1.1765 0.2268 0.2416 0.1961 0.4098 0.3205 1.3928 0.3155 0.4219 1.0707 0.3731 0.3774 0.1106 0.2114 1.0526 0.2755
LOXIMVI 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.3145 1.0787 0.2703 0.2268 0.2227 0.4132 0.3226 1.3661 0.3185 0.3690 1.0718 0.3745 0.3731 0.1170 0.2070 1.1364 0.2747
LOXIMVI 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.3484 1.3587 0.2398 0.2273 0.1946 0.4274 0.3546 1.4327 0.3497 0.4425 1.1364 0.3831 0.4425 0.1277 0.2183 1.0695 0.2597
LOXIMVI 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.2907 1.3123 0.2916 0.2336 0.2283 0.4444 0.3413 1.4225 0.3401 0.3984 1.1086 0.3731 0.4367 0.1263 0.2075 1.0941 0.2660
LOXIMVI 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.3195 1.0163 0.2445 0.2445 0.2273 0.3300 1.3605 0.3484 0.4274 1.0537 0.3846 0.4444 0.1014 0.2096 1.1351 0.2703
LOXIMVI 0.0000
uM
 5
hr
 3
Odyssey/Microvigene
 0.3937 0.9901 0.2967 0.2310 0.2155 0.4329 0.3356 1.3928 0.3333 0.3509 1.1223 0.3831 0.3677 0.1242 0.1923 1.0941 0.2874
LOXIMVI 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.3367 1.4245 0.2451 0.2481 0.2222 0.4292 0.3546 1.6103 0.3802 0.4546 1.1338 0.3802 0.4032 0.1499 0.2320 1.1274 0.2717
LOXIMVI 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.3571 1.4245 0.2646 0.2481 0.2451 0.4464 0.3390 1.4993 0.3584 0.4255 1.1001 0.3704 0.3937 0.1157 0.2110 1.1148 0.2786
LOXIMVI 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.3623 1.1287 0.2237 0.2571 0.2188 0.3534 0.3155 1.3550 0.2577 0.4049 1.0030 0.3663 0.3774 0.0917 0.2232 0.9709 0.2857
LOXIMVI 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.3677 1.1806 0.2725 0.2353 0.2326 0.3584 0.3367 1.3228 0.3030 0.3731 0.9524 0.3623 0.3571 0.1044 0.2016 1.0091 0.2833
LOXIMVI 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.3448 1.3158 0.2062 0.2445 0.2227 0.4310 0.3584 1.5408 0.3356 0.4608 1.1211 0.4082 0.4608 0.1267 0.2370 1.0526 0.2825
LOXIMVI 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.3436 1.3210 0.2660 0.2310 0.2364 0.4274 0.3846 1.5267 0.3534 0.4132 1.0428 0.3788 0.4237 0.0909 0.2222 1.0363 0.2688
LOXIMVI 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.3546 1.0650 0.2416 0.2725 0.2198 0.3704 0.3378 1.4347 0.2941 0.4032 0.9901 0.4049 0.4546 0.1109 0.2359 1.0549 0.2747
LOXIMVI 0.0000
uM
 5
hr
 4
Odyssey/Microvigene
 0.3650 1.0650 0.2571 0.2564 0.2475 0.3876 0.3788 1.3123 0.3058 0.3817 1.0787 0.3906 0.3831 0.1129 0.2110 1.0965
LOXIMVI 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.3322 1.3736 0.2387 0.2387 0.2128 0.4525 1.4837 0.3559 0.4348 1.1641 0.4329 0.4082 0.2169 1.0953 0.2809
LOXIMVI 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.3650 1.4006 0.2817 0.2755 0.2262 0.4167 0.3623 1.5015 0.3690 0.4608 1.1416 0.4049 0.4405 0.1241 0.2604 1.0373 0.2899
LOXIMVI 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.3290 1.2739 0.2451 0.2695 0.2262 0.4464 0.3650 1.3514 0.3623 0.4762 1.0616 0.4149 0.4184 0.1348 0.2252 1.0537 0.2833
LOXIMVI 0.0000
uM
 5
hr
 5
Odyssey/Microvigene
 0.3984 1.2937 0.2747 0.2865 0.2427 0.4630 0.3584 1.4085 0.3356 0.4505 1.1136 0.4149 0.3953 0.1295 0.2342 1.1249 0.3030