Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

 Full screen view
Filter results: >>>>
Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.8000 2.1186 0.1302 0.1927 0.1524 0.4255 0.1580 0.2597 1.1723 0.3745 0.3311 0.1600 0.1689 0.1543 0.3663 0.0395 0.1179 0.5525 0.5656
COLO 858 0.0000
uM
 5
hr
 6
Odyssey/Microvigene
 0.6024 1.2887 0.1312 0.2004 0.1527 0.3077 0.1704 0.2660 0.7692 0.2865 0.3300 0.1522 0.1795 0.1623 0.3584 0.0943 0.0826 0.6289 0.3948
COLO 858 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.9259 2.7624 0.1678 0.2976 0.1427 0.4587 0.1808 0.3597 1.2674 0.4444 0.5525 0.1783 0.2525 0.2058 0.3745 0.0943 0.1093 0.6098 0.6670
COLO 858 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.8265 2.2272 0.1482 0.2717 0.1639 0.4566 0.3636 1.0787 0.4049 0.4951 0.1597 0.2632 0.2421 0.4149 0.0943 0.1227 0.7407 0.5946
COLO 858 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.7353 2.1368 0.1129 0.1876 0.1163 0.4831 0.1479 0.2532 0.9259 0.3831 0.3718 0.1326 0.1695 0.1391 0.3003 0.0513 0.0980 0.4878 0.5665
COLO 858 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.6024 1.3298 0.1259 0.2033 0.1142 0.3663 0.1653 0.2695 0.7463 0.2899 0.3425 0.1407 0.1692 0.1795 0.3345 0.0730 0.0855 0.6135 0.4136
COLO 858 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.9346 2.5773 0.1511 0.2268 0.1253 0.4587 0.1560 0.2755 1.2210 0.4292 0.4630 0.1631 0.2004 0.1727 0.3546 0.0763 0.1409 0.5917 0.6697
COLO 858 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.8065 2.2936 0.1261 0.2488 0.1212 0.4566 0.1686 0.2865 0.4902 0.4184 0.4785 0.1592 0.1946 0.1972 0.3831 0.0952 0.1205 0.7194 0.5808
COLO 858 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.7692 2.1978 0.1284 0.2000 0.1590 0.4348 0.1776 0.3115 1.2422 0.3831 0.3401 0.1506 0.1812 0.1623 0.3460 0.0518 0.1126 0.5848 0.5981
COLO 858 0.0000
uM
 5
hr
 7
Odyssey/Microvigene
 0.6173 1.3228 0.1497 0.2169 0.1515 0.3425 0.1818 0.3378 0.8333 0.2924 0.3497 0.1642 0.1761 0.1712 0.3610 0.0855 0.0885 0.6757 0.4520
COLO 858 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.6250 1.7762 0.1429 0.2174 0.1287 0.3704 0.1412 0.2770 0.9174 0.3636 0.3534 0.1456 0.1812 0.1405 0.3175 0.1036 0.0813 0.4695 0.5062
COLO 858 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.5465 1.4225 0.1311 0.2128 0.1223 0.3953 0.1608 0.3115 0.7874 0.3115 0.3650 0.1520 0.1792 0.1456 0.3226 0.0641 0.0758 0.5525 0.4621
COLO 858 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.5618 1.1682 0.1486 0.2710 0.1842 0.2857 0.2024 0.3086 0.7353 0.2857 0.3876 0.1783 0.2331 0.1637 0.4329 0.0877 0.0833 0.6757 0.4130
COLO 858 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.5682 1.0695 0.1608 0.3021 0.1866 0.2762 0.1883 0.3521 0.7092 0.2525 0.4115 0.1984 0.2681 0.1866 0.4525 0.0599 0.0800 0.7299 0.4043
COLO 858 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.6135 1.6949 0.1300 0.2037 0.1429 0.3650 0.1520 0.2597 0.9434 0.3876 0.3300 0.1524 0.1848 0.1274 0.3546 0.0719 0.0917 0.4739 0.5092
COLO 858 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.5848 1.4577 0.1377 0.2151 0.1359 0.3817 0.1681 0.2865 0.8197 0.3125 0.3460 0.1618 0.1822 0.1421 0.3718 0.0725 0.1073 0.5435 0.4599
COLO 858 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.5650 1.1561 0.1468 0.2740 0.1678 0.2841 0.1869 0.2941 0.5495 0.2801 0.5348 0.1565 0.1957 0.1773 0.3663 0.0546 0.0935 0.6250 0.4269
COLO 858 0.0000
uM
 5
hr
 8
Odyssey/Microvigene
 0.5780 1.0764 0.1414 0.2681 0.1701 0.2703 0.1789 0.3215 0.8065 0.2747 0.5102 0.1698 0.2066 0.1608 0.4132 0.0800 0.0741 0.6897 0.4100
COLO 858 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 1.1236 2.7248 0.2232 0.3049 0.2083 0.3623 0.2165 0.3968 1.1390 1.1198 0.6329 0.2083 0.2786 0.3876 0.5848 0.2342 0.2217 0.7246 0.7811
COLO 858 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.9091 2.0877 0.2141 0.2899 0.2381 0.3165 0.2667 0.4525 0.9346 0.8772 0.6211 0.2020 0.2632 0.4219 0.5917 0.2110 0.1035 0.8696 0.6443
COLO 858 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 1.0000 2.2883 0.1733 0.2532 0.1715 0.2907 0.1916 0.2762 1.0070 0.9901 0.5714 0.1799 0.2041 0.4000 0.4808 0.1304 0.1425 0.6849 0.6713
COLO 858 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.6944 1.1947 0.1969 0.2571 0.1815 0.2165 0.2304 0.3003 0.6329 0.5405 0.4695 0.1905 0.2212 0.3937 0.4808 0.1605 0.0971 0.7194 0.4290
COLO 858 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 1.0050 2.5253 0.1681 0.2416 0.1359 0.2959 0.1695 0.2188 0.9346 1.1074 0.4739 0.1429 0.2110 0.2941 0.3861 0.1252 0.1742 0.5376 0.6981
COLO 858 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 0.9174 2.1598 0.1869 0.2439 0.1383 0.3145 0.1658 0.2320 0.8929 0.8547 0.4808 0.1357 0.2198 0.2994 0.3876 0.1323 0.1351 0.6250 0.6498
COLO 858 0.0000
uM
 10
hr
 1
Odyssey/Microvigene
 1.0352 2.1930 0.1412 0.2028 0.1805 0.2916 0.1701 0.2288 0.9346 0.9346 0.3521 0.1582 0.1567 0.2809 0.3534 0.0667 0.1582 0.5435 0.7100