Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)
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Small Molecule | Cell Line | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Replicate | Scanner/Software | pMEK(S217/221) | Normalized Median pMEK(S217/221) | pERK(T202/Y204) | Normalized Median pERK(T202/Y204) | p-p90RSK(S380) | Normalized Median p-p90RSK(S380) | p-p90RSK(T573) | Normalized Median p-p90RSK(T573) | p-AKT(T308) | Normalized Median p-AKT(T308) | p-AKT(S473) | Normalized Median p-AKT(S473) | p-mTOR(S2448) | Normalized Median p-mTOR(S2448) | p-p70S6K(T421/S424) | Normalized Median p-p70S6K(T421/S424) | p-p70S6K(T389) | Normalized Median p-p70S6K(T389) | p-S6(S235/236) | Normalized Median p-S6(S235/236) | p-AMPK(T172) | Normalized Median p-AMPK(T172) | p-JNK(T183/Y185) | Normalized Median p-JNK(T183/Y185) | Total c-Jun | Normalized Median Total c-Jun | p-cJun(S63) | Normalized Median p-cJun(S63) | p-P38(T180/Y182) | Normalized Median p-P38(T180/Y182) | p-HSP27(S82) | Normalized Median p-HSP27(S82) | p-NFKB(S536) | Normalized Median p-NFKB(S536) | Bim | Normalized Median Bim | cPARP | Normalized Median cPARP | p-Histone H3(S10) | Normalized Median p-Histone H3(S10) | p27 Kip1 | Normalized Median p27 Kip1 |
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Selumetinib | RVH-421 | 0.3160 | uM | 24 | hr | 2 | InnoScan/Mapix | 0.2994 | 0.5318 | 0.0626 | -3.1518 | 0.0248 | 1.4189 | 0.0268 | 1.3497 | 0.0123 | 0.7348 | 0.0181 | 0.1478 | 0.0408 | 1.3277 | 0.1508 | -0.3037 | 0.0325 | 1.0853 | 0.1226 | -0.8781 | 0.0617 | 1.4661 | 0.0173 | 1.0962 | 0.0064 | 0.1125 | 0.0195 | 0.2949 | 0.0185 | 1.3296 | 0.1028 | 1.7874 | 0.0412 | 1.2212 | 0.0869 | 4.0276 | 0.0818 | -0.3010 | 0.2375 | 1.5480 | ||
Selumetinib | RVH-421 | 0.3160 | uM | 24 | hr | 3 | InnoScan/Mapix | 0.2083 | 0.5318 | 0.0563 | -3.1518 | 1.4189 | 0.0378 | 1.3497 | 0.0172 | 0.7348 | 0.0177 | 0.1478 | 0.0492 | 1.3277 | 0.1280 | -0.3037 | 0.0399 | 1.0853 | 0.0312 | -0.8781 | 0.0367 | 1.4661 | 0.0160 | 1.0962 | 0.0046 | 0.1125 | 0.0188 | 0.2949 | 1.3296 | 0.0979 | 1.7874 | 0.0275 | 1.2212 | 0.1063 | 4.0276 | 0.0705 | -0.3010 | 0.1435 | 1.5480 | ||||
Selumetinib | RVH-421 | 0.3160 | uM | 24 | hr | 4 | InnoScan/Mapix | 0.2632 | 0.5318 | 0.0507 | -3.1518 | 0.0139 | 1.4189 | 0.0115 | 1.3497 | 0.0131 | 0.7348 | 0.0125 | 0.1478 | 0.0526 | 1.3277 | 0.1235 | -0.3037 | 0.0209 | 1.0853 | 0.1443 | -0.8781 | 0.0584 | 1.4661 | 0.0207 | 1.0962 | 0.0051 | 0.1125 | 0.0203 | 0.2949 | 0.0097 | 1.3296 | 0.0898 | 1.7874 | 0.0209 | 1.2212 | 0.1099 | 4.0276 | 0.0822 | -0.3010 | 0.1175 | 1.5480 | ||
Selumetinib | RVH-421 | 0.3160 | uM | 24 | hr | 5 | InnoScan/Mapix | 0.2359 | 0.5318 | 0.0638 | -3.1518 | 0.0261 | 1.4189 | 0.0094 | 1.3497 | 0.0144 | 0.7348 | 0.0154 | 0.1478 | 0.0340 | 1.3277 | 0.1403 | -0.3037 | 0.0253 | 1.0853 | 0.1550 | -0.8781 | 0.0437 | 1.4661 | 0.0171 | 1.0962 | 0.0062 | 0.1125 | 0.0198 | 0.2949 | 0.0113 | 1.3296 | 0.0873 | 1.7874 | 0.0263 | 1.2212 | 0.1166 | 4.0276 | 0.0539 | -0.3010 | 0.1431 | 1.5480 | ||
Selumetinib | RVH-421 | 0.3160 | uM | 24 | hr | 6 | InnoScan/Mapix | 0.2359 | 0.5318 | 0.0745 | -3.1518 | 0.0223 | 1.4189 | 0.0242 | 1.3497 | 0.0117 | 0.7348 | 0.0177 | 0.1478 | 0.0371 | 1.3277 | 0.1401 | -0.3037 | 0.0272 | 1.0853 | 0.1353 | -0.8781 | 0.0430 | 1.4661 | 0.0219 | 1.0962 | 0.0079 | 0.1125 | 0.0216 | 0.2949 | 0.0163 | 1.3296 | 0.0850 | 1.7874 | 0.0307 | 1.2212 | 0.1029 | 4.0276 | 0.0720 | -0.3010 | 0.1789 | 1.5480 | ||
Selumetinib | RVH-421 | 0.3160 | uM | 24 | hr | 7 | InnoScan/Mapix | 0.1901 | 0.5318 | 0.0329 | -3.1518 | 0.0214 | 1.4189 | 0.0239 | 1.3497 | 0.0117 | 0.7348 | 0.0134 | 0.1478 | 0.0363 | 1.3277 | 0.1311 | -0.3037 | 0.0296 | 1.0853 | 0.0416 | -0.8781 | 0.0384 | 1.4661 | 0.0106 | 1.0962 | 0.0042 | 0.1125 | 0.0133 | 0.2949 | 0.0193 | 1.3296 | 0.0903 | 1.7874 | 0.0227 | 1.2212 | 0.0875 | 4.0276 | 0.0513 | -0.3010 | 0.1149 | 1.5480 | ||
Selumetinib | RVH-421 | 0.3160 | uM | 24 | hr | 8 | InnoScan/Mapix | 0.1934 | 0.5318 | 0.0364 | -3.1518 | 0.0112 | 1.4189 | 0.0099 | 1.3497 | 0.0104 | 0.7348 | 0.0135 | 0.1478 | 0.0262 | 1.3277 | 0.1227 | -0.3037 | 0.0208 | 1.0853 | 0.1300 | -0.8781 | 0.0400 | 1.4661 | 0.0111 | 1.0962 | 0.0050 | 0.1125 | 0.0127 | 0.2949 | 0.0077 | 1.3296 | 0.0955 | 1.7874 | 0.0185 | 1.2212 | 0.0910 | 4.0276 | 0.0678 | -0.3010 | 0.0820 | 1.5480 | ||
Selumetinib | RVH-421 | 0.3160 | uM | 48 | hr | 1 | InnoScan/Mapix | 0.2632 | 0.6565 | 0.0356 | -2.7427 | 0.0204 | 1.3888 | 0.0142 | 1.8163 | 0.0196 | 1.0416 | 0.0226 | 0.3990 | 0.0458 | 1.7749 | 0.1745 | -0.0393 | 0.0346 | 1.3368 | 0.1247 | -1.6353 | 0.0575 | 1.9312 | 0.0205 | 1.4922 | 0.0055 | 0.3074 | 0.0090 | -0.5644 | 0.0138 | 1.7659 | 0.0883 | 1.5741 | 0.0350 | 1.5182 | 0.1742 | 4.0403 | 0.0666 | -0.2727 | 0.2475 | 2.1877 | ||
Selumetinib | RVH-421 | 0.3160 | uM | 48 | hr | 2 | InnoScan/Mapix | 0.2793 | 0.6565 | 0.0500 | -2.7427 | 0.0221 | 1.3888 | 0.0266 | 1.8163 | 0.0139 | 1.0416 | 0.3990 | 0.0545 | 1.7749 | 0.1709 | -0.0393 | 0.0379 | 1.3368 | 0.0286 | -1.6353 | 1.9312 | 0.0231 | 1.4922 | 0.0075 | 0.3074 | 0.0098 | -0.5644 | 0.0204 | 1.7659 | 0.0745 | 1.5741 | 0.0418 | 1.5182 | 0.0745 | 4.0403 | 0.0747 | -0.2727 | 0.2667 | 2.1877 | ||||
Selumetinib | RVH-421 | 0.3160 | uM | 48 | hr | 3 | InnoScan/Mapix | 0.1792 | 0.6565 | 0.0773 | -2.7427 | 0.0541 | 1.3888 | 0.0344 | 1.8163 | 0.0185 | 1.0416 | 0.0216 | 0.3990 | 0.0554 | 1.7749 | 0.1555 | -0.0393 | 0.0504 | 1.3368 | 0.1183 | -1.6353 | 1.9312 | 0.0229 | 1.4922 | 0.0059 | 0.3074 | 0.0129 | -0.5644 | 0.0178 | 1.7659 | 0.1063 | 1.5741 | 0.0297 | 1.5182 | 0.1050 | 4.0403 | 0.0789 | -0.2727 | 0.2217 | 2.1877 | |||
Selumetinib | RVH-421 | 0.3160 | uM | 48 | hr | 4 | InnoScan/Mapix | 0.2110 | 0.6565 | 0.0693 | -2.7427 | 0.0216 | 1.3888 | 0.0130 | 1.8163 | 0.0140 | 1.0416 | 0.0191 | 0.3990 | 0.0370 | 1.7749 | 0.1466 | -0.0393 | 0.0212 | 1.3368 | 0.1805 | -1.6353 | 0.0609 | 1.9312 | 0.0174 | 1.4922 | 0.0056 | 0.3074 | 0.0109 | -0.5644 | 0.0116 | 1.7659 | 1.5741 | 0.0243 | 1.5182 | 0.1264 | 4.0403 | -0.2727 | 0.1770 | 2.1877 | ||||
Selumetinib | RVH-421 | 0.3160 | uM | 48 | hr | 5 | InnoScan/Mapix | 0.2890 | 0.6565 | 0.0880 | -2.7427 | 0.0168 | 1.3888 | 0.0142 | 1.8163 | 0.0187 | 1.0416 | 0.0193 | 0.3990 | 0.0467 | 1.7749 | 0.1634 | -0.0393 | 0.0309 | 1.3368 | 0.0953 | -1.6353 | 0.0620 | 1.9312 | 0.0229 | 1.4922 | 0.0065 | 0.3074 | 0.0131 | -0.5644 | 0.0149 | 1.7659 | 0.0665 | 1.5741 | 0.0344 | 1.5182 | 0.1059 | 4.0403 | 0.0654 | -0.2727 | 0.2247 | 2.1877 | ||
Selumetinib | RVH-421 | 0.3160 | uM | 48 | hr | 6 | InnoScan/Mapix | 0.2899 | 0.6565 | -2.7427 | 0.0290 | 1.3888 | 0.0372 | 1.8163 | 0.0141 | 1.0416 | 0.0219 | 0.3990 | 0.0540 | 1.7749 | 0.1618 | -0.0393 | 0.0329 | 1.3368 | 0.0387 | -1.6353 | 0.0609 | 1.9312 | 1.4922 | 0.3074 | 0.0146 | -0.5644 | 0.0235 | 1.7659 | 0.0441 | 1.5741 | 0.0404 | 1.5182 | 0.0706 | 4.0403 | 0.0747 | -0.2727 | 0.2667 | 2.1877 | |||||
Selumetinib | RVH-421 | 0.3160 | uM | 48 | hr | 7 | InnoScan/Mapix | 0.2342 | 0.6565 | 0.0878 | -2.7427 | 0.0434 | 1.3888 | 0.0294 | 1.8163 | 0.0174 | 1.0416 | 0.0172 | 0.3990 | 0.0616 | 1.7749 | 0.1621 | -0.0393 | 0.0396 | 1.3368 | 0.0404 | -1.6353 | 0.0588 | 1.9312 | 0.0218 | 1.4922 | 0.0055 | 0.3074 | 0.0106 | -0.5644 | 0.0307 | 1.7659 | 0.0799 | 1.5741 | 0.0318 | 1.5182 | 0.1230 | 4.0403 | 0.0666 | -0.2727 | 0.2160 | 2.1877 | ||
Selumetinib | RVH-421 | 0.3160 | uM | 48 | hr | 8 | InnoScan/Mapix | 0.2513 | 0.6565 | 0.0745 | -2.7427 | 0.0162 | 1.3888 | 0.0253 | 1.8163 | 0.0131 | 1.0416 | 0.0191 | 0.3990 | 0.0341 | 1.7749 | 0.1672 | -0.0393 | 0.0286 | 1.3368 | 0.0585 | -1.6353 | 0.0571 | 1.9312 | 0.0185 | 1.4922 | 0.0069 | 0.3074 | 0.0087 | -0.5644 | 0.0133 | 1.7659 | 0.0776 | 1.5741 | 0.0230 | 1.5182 | 0.0936 | 4.0403 | -0.2727 | 0.1736 | 2.1877 | |||
Selumetinib | RVH-421 | 1.0000 | uM | 1 | hr | 1 | InnoScan/Mapix | 0.1484 | -0.4734 | 0.0081 | -5.9399 | 0.0139 | 0.8591 | 0.0102 | 0.5716 | 0.0137 | 0.7542 | 0.0292 | 1.0134 | 0.0272 | 0.8628 | 0.0930 | -0.8753 | 0.0261 | 0.8362 | 0.3610 | -0.0918 | 0.0339 | 0.5293 | 0.0051 | -0.2152 | 0.0036 | -0.1527 | 0.0072 | -1.0052 | 0.0049 | 0.0954 | 0.0446 | 0.1877 | 0.0161 | 0.3047 | 0.0133 | 0.2613 | 0.0909 | -0.7311 | 0.0496 | -0.1537 | ||
Selumetinib | RVH-421 | 1.0000 | uM | 1 | hr | 2 | InnoScan/Mapix | 0.1587 | -0.4734 | 0.0074 | -5.9399 | 0.0158 | 0.8591 | 0.0138 | 0.5716 | 0.0130 | 0.7542 | 0.0411 | 1.0134 | 0.0277 | 0.8628 | 0.0988 | -0.8753 | 0.0291 | 0.8362 | 0.3356 | -0.0918 | 0.0296 | 0.5293 | 0.0058 | -0.2152 | 0.0045 | -0.1527 | 0.0074 | -1.0052 | 0.0076 | 0.0954 | 0.0388 | 0.1877 | 0.0187 | 0.3047 | 0.0067 | 0.2613 | 0.1002 | -0.7311 | 0.0617 | -0.1537 | ||
Selumetinib | RVH-421 | 1.0000 | uM | 1 | hr | 3 | InnoScan/Mapix | 0.1139 | -0.4734 | 0.0130 | -5.9399 | 0.0523 | 0.8591 | 0.5716 | 0.7542 | 0.0299 | 1.0134 | 0.8628 | 0.0837 | -0.8753 | 0.0413 | 0.8362 | 0.1366 | -0.0918 | 0.0252 | 0.5293 | 0.0075 | -0.2152 | 0.0043 | -0.1527 | 0.0107 | -1.0052 | 0.0066 | 0.0954 | 0.0405 | 0.1877 | 0.0206 | 0.3047 | 0.0123 | 0.2613 | 0.0510 | -0.7311 | 0.0457 | -0.1537 | |||||
Selumetinib | RVH-421 | 1.0000 | uM | 1 | hr | 4 | InnoScan/Mapix | 0.1261 | -0.4734 | 0.0073 | -5.9399 | 0.0142 | 0.8591 | 0.0105 | 0.5716 | 0.0120 | 0.7542 | 0.0365 | 1.0134 | 0.0288 | 0.8628 | 0.0917 | -0.8753 | 0.0291 | 0.8362 | 0.2882 | -0.0918 | 0.0289 | 0.5293 | 0.0067 | -0.2152 | 0.0044 | -0.1527 | 0.0105 | -1.0052 | 0.0053 | 0.0954 | 0.0315 | 0.1877 | 0.0137 | 0.3047 | 0.0104 | 0.2613 | 0.0821 | -0.7311 | 0.0327 | -0.1537 | ||
Selumetinib | RVH-421 | 1.0000 | uM | 1 | hr | 5 | InnoScan/Mapix | 0.1074 | -0.4734 | 0.0064 | -5.9399 | 0.0116 | 0.8591 | 0.0110 | 0.5716 | 0.0129 | 0.7542 | 0.0292 | 1.0134 | 0.0229 | 0.8628 | 0.0907 | -0.8753 | 0.0168 | 0.8362 | 0.1905 | -0.0918 | 0.0203 | 0.5293 | 0.0055 | -0.2152 | 0.0037 | -0.1527 | 0.0056 | -1.0052 | 0.0045 | 0.0954 | 0.0205 | 0.1877 | 0.0121 | 0.3047 | 0.0047 | 0.2613 | 0.0446 | -0.7311 | 0.0411 | -0.1537 | ||
Selumetinib | RVH-421 | 1.0000 | uM | 1 | hr | 6 | InnoScan/Mapix | 0.1212 | -0.4734 | 0.0081 | -5.9399 | 0.0151 | 0.8591 | 0.0159 | 0.5716 | 0.0133 | 0.7542 | 0.0418 | 1.0134 | 0.0267 | 0.8628 | 0.0859 | -0.8753 | 0.0218 | 0.8362 | 0.2114 | -0.0918 | 0.0195 | 0.5293 | 0.0100 | -0.2152 | 0.0049 | -0.1527 | 0.0063 | -1.0052 | 0.0068 | 0.0954 | 0.0147 | 0.1877 | 0.0148 | 0.3047 | 0.0055 | 0.2613 | 0.0447 | -0.7311 | 0.0478 | -0.1537 | ||
Selumetinib | RVH-421 | 1.0000 | uM | 1 | hr | 7 | InnoScan/Mapix | 0.0819 | -0.4734 | 0.0087 | -5.9399 | 0.0153 | 0.8591 | 0.0177 | 0.5716 | 0.0115 | 0.7542 | 0.0180 | 1.0134 | 0.0189 | 0.8628 | 0.0815 | -0.8753 | 0.0186 | 0.8362 | 0.1499 | -0.0918 | 0.0141 | 0.5293 | 0.0076 | -0.2152 | 0.0040 | -0.1527 | 0.0084 | -1.0052 | 0.0033 | 0.0954 | 0.0280 | 0.1877 | 0.0119 | 0.3047 | 0.0088 | 0.2613 | 0.0430 | -0.7311 | 0.0424 | -0.1537 | ||
Selumetinib | RVH-421 | 1.0000 | uM | 1 | hr | 8 | InnoScan/Mapix | 0.0903 | -0.4734 | 0.0115 | -5.9399 | 0.8591 | 0.0082 | 0.5716 | 0.0099 | 0.7542 | 0.0244 | 1.0134 | 0.0166 | 0.8628 | 0.0991 | -0.8753 | 0.0122 | 0.8362 | 0.2242 | -0.0918 | 0.0143 | 0.5293 | -0.2152 | -0.1527 | 0.0098 | -1.0052 | 0.0038 | 0.0954 | 0.0257 | 0.1877 | 0.0076 | 0.3047 | 0.0035 | 0.2613 | 0.0517 | -0.7311 | 0.0243 | -0.1537 | |||||
Selumetinib | RVH-421 | 1.0000 | uM | 5 | hr | 1 | InnoScan/Mapix | 0.2639 | 0.6994 | 0.0186 | -4.9504 | 0.0112 | 0.9273 | 0.0113 | 1.1135 | 0.0120 | 0.6322 | 0.0161 | 0.4546 | 0.0234 | 0.9042 | 0.1346 | -0.3627 | 0.0332 | 0.9777 | 0.1783 | -1.0365 | 0.0452 | 1.5261 | 0.0063 | -0.4177 | 0.0036 | -0.4877 | 0.0068 | -1.4783 | 0.0104 | 1.1187 | 0.0591 | 0.9717 | 0.0224 | 0.8831 | 0.0274 | 1.4833 | 0.2681 | 1.3813 | 0.0835 | 0.7153 | ||
Selumetinib | RVH-421 | 1.0000 | uM | 5 | hr | 2 | InnoScan/Mapix | 0.2825 | 0.6994 | 0.0189 | -4.9504 | 0.0179 | 0.9273 | 0.0159 | 1.1135 | 0.0103 | 0.6322 | 0.0204 | 0.4546 | 0.0313 | 0.9042 | 0.1316 | -0.3627 | 0.0263 | 0.9777 | 0.1825 | -1.0365 | 0.0437 | 1.5261 | 0.0072 | -0.4177 | 0.0046 | -0.4877 | 0.0067 | -1.4783 | 0.0147 | 1.1187 | 0.0532 | 0.9717 | 0.0267 | 0.8831 | 0.0149 | 1.4833 | 0.2985 | 1.3813 | 0.0928 | 0.7153 |