Cor.4U - Differentiated Cell (ID:75001)

HMS LINCS ID: 75001
Name: Cor.4U
Alternative Names: Cor.4U human induced Pluripotent Stem Cell-derived cardiomyocytes (hiPSC-CMs)
LINCS ID:
Alternative ID:
Precursor iPSC Cell: HMSL70001-1: Axiogenesis iPSC
Details of Cell Type: cardiomyocyte
Cell Type Detail: mix of sub-types: atrial, ventricular, nodal
Known Mutations:
Citation Information for Mutations:
Molecular Features:
Recommended Culture Conditions: Complete culture medium recipe:
450mL IMDM (PAN,P04-20150)
500μL Insulin (Insulin solution human, I9278, Sigma)
50mL Fetal calf serum (heat inactivated)
Thioglycerol (400 μM)
BMCC serum free culture medium recipe:
500ml IMDM (PAN,P04-20150)
500μl Insulin (Insulin solution human, I9278, Sigma)
5ml CD-Lipids (CD Lipids concentrate, 11905-031, Gibco)
500μl Trace Elements A ( 25-021-CI, Cellgro)
500μl Trace Elements B ( 99-175-CI, Cellgro)
500μl Trace Elements C (99-176-CI, Cellgro)
250μl Vitamin C ( 100mg/ml Stock)
Thioglycerol (400 μM)
Relevant Citations:
Related Projects:
Cell Markers: express cardiac-specific proteins, e.g. cardiac alpha-actinin and the gap junction protein connexin-43
Genetic Modification(s):
Differentiation Protocol: proprietary protocol
Comments:
Date Publicly Available: 2017-09-29
Most Recent Update: 2017-09-29

Datasets:

HMS Dataset ID Dataset Title HMS Dataset Type
20324
Tyrosine Kinase Inhibitor-induced cardiotoxicity in hiPSC derived cardiomyocytes - pilot dataset
RNA-Seq
20325
Tyrosine Kinase Inhibitor-induced cardiotoxicity in hiPSC derived cardiomyocytes - pilot dataset (qPCR validation)
qPCR
20349
Tyrosine Kinase Inhibitor-induced cardiotoxicity in hiPSC derived cardiomyocytes - Proteomics
Proteomics

Batch Information:

HMS LINCS Batch ID Provider Provider Catalog ID Provider Batch ID
75001-1 Ncardia, previously Axiogenesis Ax-B-HC02-4M CB188CL_V_4M
75001-2 Ncardia, previously Axiogenesis Ax-B-HC02-4M CB212CL_V_4M
75001-3 Ncardia, previously Axiogenesis Ax-B-HC02-4M CB254CL_V_4M
75001-4 Ncardia, previously Axiogenesis Ax-B-HC02-4M CB302CL_V_4M
75001-5 Ncardia, previously Axiogenesis Ax-B-HC02-4M F2015-KW19CL1
75001-6 Ncardia, previously Axiogenesis Ax-B-HC02-4M F2015-KW20CL2