Cor.4U - Differentiated Cell (ID:75001)

HMS LINCS ID: 75001
Name: Cor.4U
Alternative Names: Cor.4U human induced Pluripotent Stem Cell-derived cardiomyocytes (hiPSC-CMs)
LINCS ID:
Alternative ID:
Precursor iPSC Cell: HMSL70001-1: Axiogenesis iPSC
Details of Cell Type: cardiomyocyte
Cell Type Detail: mix of sub-types: atrial, ventricular, nodal
Known Mutations:
Citation Information for Mutations:
Molecular Features:
Recommended Culture Conditions: Complete culture medium recipe:
450mL IMDM (PAN,P04-20150)
500μL Insulin (Insulin solution human, I9278, Sigma)
50mL Fetal calf serum (heat inactivated)
Thioglycerol (400 μM)
BMCC serum free culture medium recipe:
500ml IMDM (PAN,P04-20150)
500μl Insulin (Insulin solution human, I9278, Sigma)
5ml CD-Lipids (CD Lipids concentrate, 11905-031, Gibco)
500μl Trace Elements A ( 25-021-CI, Cellgro)
500μl Trace Elements B ( 99-175-CI, Cellgro)
500μl Trace Elements C (99-176-CI, Cellgro)
250μl Vitamin C ( 100mg/ml Stock)
Thioglycerol (400 μM)
Relevant Citations:
Related Projects:
Cell Markers: express cardiac-specific proteins, e.g. cardiac alpha-actinin and the gap junction protein connexin-43
Genetic Modification(s):
Differentiation Protocol: proprietary protocol
Comments:
Date Publicly Available: 2017-09-29
Most Recent Update: 2017-09-29

Batch Information for HMSL75001-2:

HMS LINCS Batch ID: 75001-2
Provider: Ncardia, previously Axiogenesis
Provider Catalog ID: Ax-B-HC02-4M
Provider Batch ID: CB212CL_V_4M
Source Information:
Date Received:
HMS QC Outcome:
Culture Conditions: Complete culture medium recipe:
450mL IMDM (PAN,P04-20150)
500μL Insulin (Insulin solution human, I9278, Sigma)
50mL Fetal calf serum (heat inactivated)
Thioglycerol (400 μM) BMCC serum free culture medium recipe:
500ml IMDM (PAN,P04-20150)
500μl Insulin (Insulin solution human, I9278, Sigma)
5ml CD-Lipids (CD Lipids concentrate, 11905-031, Gibco)
500μl Trace Elements A ( 25-021-CI, Cellgro)
500μl Trace Elements B ( 99-175-CI, Cellgro)
500μl Trace Elements C (99-176-CI, Cellgro)
250μl Vitamin C ( 100mg/ml Stock)
Thioglycerol (400 μM)
1% FBS
Passage Number:
Days Post Differentiation:
Transient Modification(s): none
Comments:
Date Publicly Available: 2017-09-29
Most Recent Update: 2018-03-20