Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 2 of 4) - Dataset (ID:20275)

Data Columns

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Name Description Display Name Data Type Unit Precision Readout Type Replicate Comments
meanFluorescenceIntensity488
Mean fluorescence intensity at wavelength 488 nm
Mean Fluorescence Intensity (488) Numeric arbitrary unit 2
meanFluorescenceIntensity568
Mean fluorescence intensity at wavelength 568 nm
Mean Fluorescence Intensity (568) Numeric arbitrary unit 2
meanFluorescenceIntensity647
Mean fluorescence intensity at wavelength 647 nm
Mean Fluorescence Intensity (647) Numeric arbitrary unit 2
timePointUnit Time Point Unit Text hr
concUnit Small Mol Conc Unit Text uM
protein1Detected647
Protein 1 detected by immunofluorescence at wavelength 647 nm
Protein 1 Detected (647) protein
secondaryAntibody647
Secondary antibody reagent for detection at wavelength 647 nm
Secondary Antibody (647) antibody
primaryAntibody647
Primary antibody reagent for detection at wavelength 647 nm
Primary Antibody (647) antibody
proteinDetected568
Protein detected by immunofluorescence at wavelength 568 nm
Protein Detected (568) protein
secondaryAntibody568
Secondary antibody reagent for detection at wavelength 568 nm
Secondary Antibody (568) antibody
primaryAntibody568
Primary antibody reagent for detection at wavelength 568 nm
Primary Antibody (568) antibody
proteinDetected488
Protein detected by immunofluorescence at wavelength 488 nm
Protein Detected (488) protein
secondaryAntibody488
Secondary antibody reagent for detection at wavelength 488 nm
Secondary Antibody (488) antibody
primaryAntibody488
Primary antibody reagent for detection at wavelength 488 nm
Primary Antibody (488) antibody
replicate Replicate Numeric 0
timePoint Time Point Numeric 0
drugConcentration2 Small Mol 2 Concentration Numeric 3
drugConcentration1 Small Mol 1 Concentration Numeric 3
smallMolecule2
Small molecule perturbagen
Small Molecule 2 small_molecule
smallMolecule1
Small molecule perturbagen
Small Molecule 1 small_molecule
plateWell Plate Well Text
cell
Cell reagent
Cell cell
plateName Plate Name Text
protein4Detected647
Protein 4 detected by immunofluorescence at wavelength 647 nm
Protein 4 Detected (647) protein
protein3Detected647
Protein 3 detected by immunofluorescence at wavelength 647 nm
Protein 3 Detected (647) protein
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