Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 2 of 4) - Dataset (ID:20275)

Data Columns

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Name Description Display Name Data Type Unit Precision Readout Type Replicate Comments
cell
Cell reagent
Cell cell
meanFluorescenceIntensity488
Mean fluorescence intensity at wavelength 488 nm
Mean Fluorescence Intensity (488) Numeric arbitrary unit 2
meanFluorescenceIntensity568
Mean fluorescence intensity at wavelength 568 nm
Mean Fluorescence Intensity (568) Numeric arbitrary unit 2
meanFluorescenceIntensity647
Mean fluorescence intensity at wavelength 647 nm
Mean Fluorescence Intensity (647) Numeric arbitrary unit 2
plateName Plate Name Text
plateWell Plate Well Text
primaryAntibody488
Primary antibody reagent for detection at wavelength 488 nm
Primary Antibody (488) antibody
primaryAntibody568
Primary antibody reagent for detection at wavelength 568 nm
Primary Antibody (568) antibody
primaryAntibody647
Primary antibody reagent for detection at wavelength 647 nm
Primary Antibody (647) antibody
protein1Detected647
Protein 1 detected by immunofluorescence at wavelength 647 nm
Protein 1 Detected (647) protein
protein2Detected647
Protein 2 detected by immunofluorescence at wavelength 647 nm
Protein 2 Detected (647) protein
protein3Detected647
Protein 3 detected by immunofluorescence at wavelength 647 nm
Protein 3 Detected (647) protein
protein4Detected647
Protein 4 detected by immunofluorescence at wavelength 647 nm
Protein 4 Detected (647) protein
proteinDetected488
Protein detected by immunofluorescence at wavelength 488 nm
Protein Detected (488) protein
proteinDetected568
Protein detected by immunofluorescence at wavelength 568 nm
Protein Detected (568) protein
replicate Replicate Numeric 0
secondaryAntibody488
Secondary antibody reagent for detection at wavelength 488 nm
Secondary Antibody (488) antibody
secondaryAntibody568
Secondary antibody reagent for detection at wavelength 568 nm
Secondary Antibody (568) antibody
secondaryAntibody647
Secondary antibody reagent for detection at wavelength 647 nm
Secondary Antibody (647) antibody
drugConcentration1 Small Mol 1 Concentration Numeric 3
drugConcentration2 Small Mol 2 Concentration Numeric 3
concUnit Small Mol Conc Unit Text uM
smallMolecule1
Small molecule perturbagen
Small Molecule 1 small_molecule
smallMolecule2
Small molecule perturbagen
Small Molecule 2 small_molecule
timePoint Time Point Numeric 0
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