Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 101.95 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 120.37
WM115-P4
G10
WM-115 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 331.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 96.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 122.36
WM115-P4
G11
WM-115 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 330.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 78.62 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 180.73
WM115-P4
G2
WM-115 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 399.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 87.77 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 201.01
WM115-P4
G3
WM-115 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 287.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 83.00 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 130.39
WM115-P4
G4
WM-115 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 284.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 85.15 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 125.68
WM115-P4
G5
WM-115 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 85.67 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 187.41
WM115-P4
G6
WM-115 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 249.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 88.02 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 160.63
WM115-P4
G7
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 235.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 87.45 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 154.60
WM115-P4
G8
WM-115 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 88.10 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 97.08
WM115-P4
G9
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 319.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.20 NGFR rabbit IgG(H+L) / donkey NGFR 1378.93
WM115-P7
B10
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 199.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 101.49 NGFR rabbit IgG(H+L) / donkey NGFR 1348.46
WM115-P7
B11
WM-115 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1228.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 363.06 NGFR rabbit IgG(H+L) / donkey NGFR 1118.25
WM115-P7
B2
WM-115 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 882.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 211.51 NGFR rabbit IgG(H+L) / donkey NGFR 993.83
WM115-P7
B3
WM-115 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 751.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 186.48 NGFR rabbit IgG(H+L) / donkey NGFR 1206.66
WM115-P7
B4
WM-115 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 656.54 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.09 NGFR rabbit IgG(H+L) / donkey NGFR 1005.59
WM115-P7
B5
WM-115 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1060.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.41 NGFR rabbit IgG(H+L) / donkey NGFR 1143.08
WM115-P7
B6
WM-115 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1091.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 202.38 NGFR rabbit IgG(H+L) / donkey NGFR 1156.82
WM115-P7
B7
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1071.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 277.53 NGFR rabbit IgG(H+L) / donkey NGFR 1202.27
WM115-P7
B8
WM-115 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 906.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 259.11 NGFR rabbit IgG(H+L) / donkey NGFR 1044.06
WM115-P7
B9
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 100.46 NGFR rabbit IgG(H+L) / donkey NGFR 1930.11
WM115-P7
C10
WM-115 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 255.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 98.68 NGFR rabbit IgG(H+L) / donkey NGFR 1691.78
WM115-P7
C11
WM-115 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 651.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.57 NGFR rabbit IgG(H+L) / donkey NGFR 1715.91
WM115-P7
C2
WM-115 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 548.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.68 NGFR rabbit IgG(H+L) / donkey NGFR 1716.12
WM115-P7
C3
WM-115 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 570.96 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 128.44 NGFR rabbit IgG(H+L) / donkey NGFR 1351.44
WM115-P7
C4