Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 564.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 81.46 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 16.36
WM115-P8
D7
WM-115 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 722.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 100.26 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 25.70
WM115-P8
C7
WM-115 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 794.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 101.97 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 27.50
WM115-P8
F7
WM-115 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 717.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 113.19 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 27.56
WM115-P8
G7
WM-115 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 617.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 86.86 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 28.01
WM115-P8
G6
WM-115 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 514.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 89.87 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 30.79
WM115-P8
D6
WM-115 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 513.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 89.69 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 31.49
WM115-P8
G5
WM-115 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 369.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 102.23 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 32.01
WM115-P8
D5
COLO 858 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 365.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 128.93 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 36.97
Colo858-P8
G7
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 291.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 80.39 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 38.02
WM115-P10
C9
COLO 858 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 335.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 128.69 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 38.37
Colo858-P8
G6
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 314.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 79.73 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 39.13
WM115-P10
G9
WM-115 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 629.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 98.77 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 39.54
WM115-P8
C6
WM-115 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 832.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.42 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 39.82
WM115-P8
E7
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 300.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 89.31 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 40.05
WM115-P10
D9
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 86.32 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 42.90
WM115-P10
F9
WM-115 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 456.83 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 93.07 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 43.23
WM115-P8
F5
COLO 858 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 356.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 124.14 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 43.40
Colo858-P8
F7
COLO 858 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.06 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 43.62
Colo858-P10
C9
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.64 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 45.05
Colo858-P10
D9
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 631.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 190.51 NGFR rabbit IgG(H+L) / donkey NGFR 45.27
Colo858-P1
D11
WM-115 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 958.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.39 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 46.78
WM115-P8
B7
WM-115 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 491.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.62 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 47.00
WM115-P8
C5
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 623.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 215.11 NGFR rabbit IgG(H+L) / donkey NGFR 47.15
Colo858-P1
G11
COLO 858 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 479.96 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 164.91 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 47.87
Colo858-P8
G5
  • Page 1 of 58
  • 1 to 25 of 1440 records