HMS LINCS Batch ID,HMS LINCS ID,Name,Alternative Names,LINCS ID,Alternative ID,Reference Source,Organism,Organ,Tissue,Cell Type,Details of Cell Type,Donor Sex,Donor Age,Donor Ethnicity,Donor Health Status,Disease,Details of Disease,Production Details,Genetic Modification(s),Known Mutations,Citation Information for Mutations,Verification Reference Profile,Growth Properties,Recommended Culture Conditions,Relevant Citations,Usage Note,Comments,Provider,Provider Catalog ID,Provider Batch ID,Source Information,Date Received,HMS QC Outcome,Transient Modification(s),Date Publicly Available,Most Recent Update
50060-2,50060,A-375,A375,LCL-1235,"CLO_0001582","ATCC CRL-1619",Homo sapiens,skin,,epithelial,,female,,,,"DOID:1909, malignant melanoma",,,none,,"COSS906793","DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 8
TPOX: 8,10
vWA: 16,17",adherent,"ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0 deg C
Subculturing protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37 deg C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days",,,,Nienke Moret (Harvard Medical School),,,"Obtained by Nienke Moret (Harvard Medical School) from ATCC on September 16, 2014, and distributed to Mohammad Fallahi-Sichani in April, 2015.",2014-04,,,2012-05-01,2016-07-12
50149-4,50149,COLO 858,,LCL-1242,"CLO_0002569","European Collection of Authenticated Cell Cultures (ECACC) 93052613",Homo sapiens,skin,,,,male,,,,"DOID:1909, malignant melanoma",,,none,,,,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37C; 5% CO2.",,,,Nathan Moerke (Harvard Medical School),,,Obtained by Nathan Moerke (Harvard Medical School) from the Center for Molecular Therapeutics (CMT; Massachusetts General Hospital) (contact: Cyril Benes) and distributed to Mohammad Fallahi-Sichani in 2012,2012,,,2012-07-31,2016-08-24
50567-3,50567,WM-115,,LCL-1260,"CLO_0009613","ATCC CRL-1675",Homo sapiens,skin,,,,female,,,,"DOID:1909, malignant melanoma",,,none,,"COSS909784",,adherent,"From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.03% EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 34 to 35°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 34 to 35°C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended",,,,Nathan Moerke (Harvard Medical School),,,Obtained by Nathan Moerke (Harvard Medical School) from the Center for Molecular Therapeutics (CMT; Massachusetts General Hospital) (contact: Cyril Benes) and distributed to Mohammad Fallahi-Sichani in 2012,2012,,,2012-07-31,2016-04-04
50999-3,50999,MMAC-SF,,LCL-1277,"CLO_0037131","Cell Bank, RIKEN BioResource Center RCB1200",Homo sapiens,skin,,,,na,,,,"DOID:1909, malignant melanoma",malignant melanoma; ascites,,none,,"COSS925339",,adherent,,,,,Nathan Moerke (Harvard Medical School),,,Obtained by Nathan Moerke (Harvard Medical School) from the Center for Molecular Therapeutics (CMT; Massachusetts General Hospital) (contact: Cyril Benes) and distributed to Mohammad Fallahi-Sichani in 2012. CMT previously had obtained the cells from the Wellcome Trust Sanger Institute.,2012,,,2012-07-31,2016-07-12
51006-3,51006,MZ7-mel,,LCL-1279,"CLO_0037133",Thomas Woelfel (Johannes Gutenberg University Mainz),Homo sapiens,skin,,,,female,,,,"DOID:1909, malignant melanoma",,,none,,"COSS753596",,adherent,,,,,Nathan Moerke (Harvard Medical School),,,Obtained by Nathan Moerke (Harvard Medical School) from the Center for Molecular Therapeutics (CMT; Massachusetts General Hospital) (contact: Cyril Benes) and distributed to Mohammad Fallahi-Sichani in 2012. CMT previously had obtained the cells from the Wellcome Trust Sanger Institute.,2012,,,2012-07-31,2016-07-12