Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

 Full screen view
Filter results: >>>>
Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 399.6 4
0306-0623
 19.85 623.15 257 57.94 0.962
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 780.7 4
0307-0626
 446.30 626.23 296 65.84 0.858
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 315.4 4
0308-0628
 276.95 627.68 296 65.60 0.864
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1556.8 4
0309-0632
 393.23 631.71 281 63.60 0.873
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1667.2 4
0310-0629
 712.87 629.97 259 61.36 0.865
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2841.8 4
0311-0633
 197.52 634.07 279 62.53 0.897
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3669.8 4
0312-0642
 488.06 641.33 754 112.47 0.749
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1260.9 4
0313-0637
 829.47 636.54 263 61.26 0.881
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3620.0 4
0314-0644
 345.03 644.26 229 55.70 0.928
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 391.2 4
0315-0641
 608.85 641.57 282 65.36 0.830
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4214.9 4
0316-0647
 814.22 647.40 352 70.67 0.886
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1296.1 4
0317-0648
 411.99 648.90 283 67.84 0.773
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1117.2 4
0318-0654
 217.20 652.52 594 101.74 0.721
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 7452.4 4
0319-0647
 372.52 646.90 117 54.67 0.492
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 5743.8 4
0320-0651
 366.21 653.00 129 56.43 0.509
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2038.1 4
0321-0657
 751.75 656.75 276 64.77 0.827
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 687.7 4
0322-0661
 29.53 660.50 527 89.26 0.831
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 301.3 4
0323-0658
 948.14 657.79 286 63.60 0.889
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 384.3 4
0324-0664
 526.90 664.58 255 62.53 0.820
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 963.0 4
0325-0661
 625.34 660.88 249 57.94 0.932
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 335.7 4
0326-0664
 562.53 664.41 309 68.43 0.829
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1141.6 4
0327-0667
 784.11 667.20 269 63.36 0.842
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 323.2 4
0328-0665
 139.16 664.90 285 63.36 0.892
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1199.9 4
0329-0670
 586.29 670.21 256 59.94 0.895
COLO 858 Vemurafenib
0.0316
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 5305.8 4
0330-0670
 914.77 670.05 225 54.87 0.939