Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

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Cell Small Molecule Small Mol Concentration Small Mol Conc Unit Timepoint Timepoint Unit Raw Image Registered Image CycIF Round Primary Antibody Secondary Antibody Cytell filter Protein Detected Other Reagent Detected Measured Fluorescence Intensity (a.u.) Field Cell ID Number X-coordinate Y-coordinate Cell Area (um^2) Cell Perimeter (um) Cell Circularity
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2253.8 8
0322-0523
 544.13 524.15 269 79.01 0.541
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1133.7 8
0323-0524
 373.61 524.93 246 58.77 0.895
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1634.1 8
0324-0524
 249.55 524.74 219 54.87 0.914
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1492.3 8
0325-0528
 587.81 528.57 220 57.26 0.843
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1441.8 8
0326-0528
 661.83 528.57 227 57.11 0.875
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4605.5 8
0327-0529
 741.26 528.96 331 66.18 0.950
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4055.2 8
0328-0529
 265.76 529.33 168 49.11 0.875
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4487.1 8
0329-0530
 886.67 530.57 195 53.36 0.861
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 3195.9 8
0330-0536
 475.34 535.73 334 73.36 0.780
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 805.5 8
0331-0532
 693.26 532.27 262 62.77 0.836
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2044.0 8
0332-0535
 904.34 535.08 217 57.11 0.836
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2059.1 8
0333-0540
 503.66 539.29 356 73.01 0.839
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4571.1 8
0334-0536
 837.33 536.46 168 51.11 0.808
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 785.9 8
0335-0540
 618.73 540.15 265 63.60 0.823
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 334.1 8
0336-0545
 108.17 545.82 246 60.77 0.837
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 10435.3 8
0337-0540
 310.74 540.38 112 45.11 0.692
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2334.4 8
0338-0544
 540.04 543.04 278 84.91 0.485
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1392.3 8
0339-0543
 647.59 542.83 260 59.94 0.909
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2215.7 8
0340-0543
 228.87 543.02 213 53.94 0.920
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 7958.4 8
0341-0547
 440.74 547.05 393 74.18 0.897
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2330.6 8
0342-0547
 294.62 547.99 340 68.53 0.910
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 4708.7 8
0343-0549
 63.74 548.50 494 85.84 0.842
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1814.0 8
0344-0547
 405.03 547.38 199 53.94 0.859
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 2285.2 8
0345-0553
 720.38 553.08 424 78.91 0.856
COLO 858 Vemurafenib
0
uM
 48
hr
 image if available image if available
Cycle-1
 KI-67 / clone D3B5 rabbit IgG(H+L) / goat / cross-adsorbed
FITC
 KI-67 1302.6 8
0346-0555
 585.25 555.47 238 57.11 0.917