HMS LINCS ID,Name,Alternative Names,LINCS ID,Alternative ID,Parent Cell Line,Parent Cell Line HMS LINCS ID,Reference Source,Organism,Organ,Tissue,Cell Type,Details of Cell Type,Donor Sex,Donor Age,Donor Ethnicity,Donor Health Status,Disease,Details of Disease,Production Details,Genetic Modification(s),Known Mutations,Citation Information for Mutations,Verification Reference Profile,Growth Properties,Recommended Culture Conditions,Relevant Citations,Usage Note,Comments,Date Publicly Available,Most Recent Update
50001,5637,,LCL-1702,"CLO_0001421",,,"ATCC HTB-9",Homo sapiens,urinary bladder,,epithelial,,male,,,,"DOID:4007, bladder carcinoma",,,none,,"COSS687452","DNA Profile (STR, source: ATCC):
Amelogenin: X,Y
CSF1PO: 11
D13S317: 11
D16S539: 9
D5S818: 11,12
D7S820: 10,11
THO1: 7,9
TPOX: 8,9
vWA: 16,18",adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:8 is recommended",,,,2012-04-25,2016-04-04
50002,647-V,,LCL-1708,"CLO_0001450",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 414",Homo sapiens,urinary bladder,,epithelial-like,,male,,,,"DOID:4007, bladder carcinoma; DOID:4006, bladder transitional cell carcinoma",transitional cell carcinoma; urothelial bladder carcinoma,,none,,"COSS906797",,adherent,From MGH/CMT as specified by cell provider: 85% Dulbecco's MEM + 15% FBS + 2 mM L-glutamine split confluent culture 1:5 to 1:6 every 3-4 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm 2 in 10 ml medium at 37C with 5-10% CO2 cell harvest of ca. 10-15 x 106 cells/175 cm 2,,,,2012-04-25,2016-08-24
50003,A375.S2,,LCL-1231,"CLO_0001583",,,"ATCC CRL-1872",Homo sapiens,skin,,epithelial,,female,,,,"DOID:1909, malignant melanoma",,,none,,,"DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 8
TPOX: 8,10
vWA: 16,17",adherent,"From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Subculturing: Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution and let the culture sit at room temperature (or 37C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended",,,,2012-04-25,2016-07-12
50004,AGS,,LCL-1893,"CLO_0001686",,,"ATCC CRL-1739",Homo sapiens,stomach,,epithelial,,female,,,,"DOID:5517, stomach carcinoma; DOID:3717, gastric adenocarcinoma",,,none,,"COSS906790",,adherent,"From MGH/CMT as specified by cell provider: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 90%; fetal bovine serum, 10%",,,,2012-04-25,2016-04-04
50005,BPH-1,,LCL-2095,"CLO_0002022",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 143",Homo sapiens,prostate,,epithelioid,,male,,,,"DOID:0060087, male reproductive benign neoplasm",benign prostate hyperplasia,,none,,"COSS924105",,adherent,From MGH/CMT as specified by cell provider: 80% RPMI 1640 + 20% FBS + 20 ng/ml testosterone + 5 microg/ml transferrin + 5 ng/ml sodium selenite + 5 microg/ml insulin + trace elements mix (a list can be provided as they are no longer commercially available in a single mix) split confluent culture 1:3 to 1:8 every 3-7 days using trypsin/EDTA (cells should be incubated for more than 10 min with trypsin/EDTA at 37C); seed out at 0.1 x 10 5 cells/cm 2 ; we noted that the cells grow equally well without the trace element mix which was recommended by the depositor at 37C with 5% CO2 cell harvest of ca. 0.4 x 105 cells/cm 2,,,,2012-04-25,2016-08-24
50006,Ca Ski,CaSki,LCL-1541,"CLO_0002165",,,"ATCC CRL-1550",Homo sapiens,cervix,,epithelial,,female,,,,"DOID:3744, cervical squamous cell carcinoma",,,none,,"COSS906824","STR-PCR Data (source: ECACC):
Amelogenin: X
CSF1PO: 10
D13S317: 8,12
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 7
TPOX: 8
vWA: 17",adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.",,,,2012-04-25,2016-07-12
50007,Ca9-22,,LCL-2033,"CLO_0051380",,,"Japanese Collection of Research Bioresources Cell Bank JCRB0625",Homo sapiens,head & neck,,epithelial-like,,male,,,,"DOID:8602, malignant gingival tumor",gingival carcinoma,,none,,"COSS753538",,not specified,From MGH/CMT as specified by cell provider: EMEM w/ 0.6g/l glut and 10% FCS,,,,2012-04-25,2016-07-12
50008,CAL-51,,LCL-1472,"CLO_0002185",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 302",Homo sapiens,breast,,epithelial-like,,female,,,,"DOID:3459, breast carcinoma",,,none,,"COSS910927",,adherent,From MGH/CMT as specified by cell provider: 80% Dulbecco's MEM (4.5 g/L glucose) + 20% FBS split culture 1:2 once or twice a week using trypsin/EDTA before cells reach complete confluency; seed out at ca. 2 x 106 cells/25 cm 2 ; cells may grow very slowly initially after thawing or after stringent trypsination at 37C with 5-10% CO2 cell harvest of ca. 15-20 x 106 cells/80 cm 2,,,,2012-04-25,2016-08-24
50009,Calu-1,,LCL-1580,"CLO_0002191",,,"ATCC HTB-54",Homo sapiens,lung,,epithelial,,male,,,,"DOID:3907, epidermoid lung carcinoma",NSCLC epidermoid lung carcinoma,,none,,,"DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 11
D5S818: 10,12
D7S820: 9,10
THO1: 9,9.3
TPOX: 8
vWA: 15,16",adherent,"From MGH/CMT as specified by cell provider: McCoy's 5a medium (modified) with 1.5 mM L-glutamine adjusted to contain 2.2 g/L sodium bicarbonate, 90%; fetal bovine serum, 10%",,,,2012-04-25,2016-07-12
50010,Calu-3,,LCL-1631,"CLO_0002192",,,"ATCC HTB-55",Homo sapiens,lung,,epithelial,,male,,,,"DOID:3910, lung adenocarcinoma",NSCLC adenocarcinoma,,none,,"COSS687777","DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 12,14
D5S818: 11
D7S820: 10,11
THO1: 6,9.3
TPOX: 8
vWA: 16,17",adherent,"From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%",,,,2012-04-25,2016-04-04
50011,COLO-679,,LCL-1232,"CLO_0002551",,,"Cell Bank, RIKEN BioResource Center RCB0989",Homo sapiens,skin,,fibroblastic,,female,,,,"DOID:1909, malignant melanoma",,,none,,"COSS906818",,adherent,From MGH/CMT as specified by cell provider: 90-95% RPMI 1640 + 5-10% FBS split confluent culture 1:5 to 1:10 using trypsin/EDTA; change medium twice weekly; seed out at ca. 2 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of about 25 x 106 cells/175 cm 2,,,,2012-04-25,2016-04-04
50012,COLO-800,,LCL-1233,"CLO_0002561",,,"European Collection of Authenticated Cell Cultures (ECACC) 93051123",Homo sapiens,skin,,epithelial-like,,male,,,,"DOID:1909, malignant melanoma",,,none,,"COSS906813",,adherent,From MGH/CMT as specified by cell provider: 90-95% RPMI 1640 + 5-10% FBS split confluent culture 1:4 to 1:5 two to three times per week using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of about 5 x 106 cells/80 cm 2,,,,2012-04-25,2016-08-24
50013,FU97,,LCL-1894,"CLO_0037138",,,"Japanese Collection of Research Bioresources Cell Bank JCRB1074",Homo sapiens,stomach,,epithelial-like,,female,,,,"DOID:5517, stomach carcinoma; DOID:3717, gastric adenocarcinoma",,,none,,"COSS1290806",,adherent,From MGH/CMT as specified by cell provider: Dulbecco's modified Eagle's medium (4.5g/L glucose) with 10% fetal calf serum and 10mg/L insulin,,,,2012-04-25,2016-07-12
50014,HEC-1,,LCL-1496,"CLO_0037162",,,"Japanese Collection of Research Bioresources Cell Bank JCRB0042",Homo sapiens,uterus,,,,female,,,,"DOID:363, uterine cancer; DOID:1380, Endometrial cancer; DOID:2871, endometrial carcinoma; DOID:2870, endometrial adenocarcinoma",,,none,,"COSS907051",,not specified,JHSF growth medium: Eagle's minimal essential medium with 10% calf serum.,,,,2012-04-25,2016-07-12
50015,HLF,,LCL-1938,"CLO_0009987",,,"Japanese Collection of Research Bioresources Cell Bank JCRB0405",Homo sapiens,liver,,,,male,,,,"DOID:684, hepatoma",hepatocellular carcinoma,,none,,,,adherent,From MGH/CMT as specified by cell provider: DMEM w/ 5% FBS,,,,2012-04-25,2016-07-12
50016,HUTU-80,HuTu 80,LCL-1150,"CLO_0004305",,,"ATCC HTB-40",Homo sapiens,intestine,,epithelial,,male,,,,"DOID:10816, duodenum adenocarcinoma",,,none,,"COSS907073","DNA Profile (STR, source: ATCC):
Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 8,11
D16S539: 10,11
D5S818: 12,13
D7S820: 9,11
THO1: 7
TPOX: 9,11
vWA: 16,18",adherent,"ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing: Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37 deg C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended.",,,,2012-04-25,2016-04-04
50017,IA-LM,,LCL-1778,"CLO_0050075",,,"Cell Bank, RIKEN BioResource Center RCB0554",Homo sapiens,lung,,,,male,,,,"DOID:4556, lung large cell carcinoma","NSCLC large cell carcinoma, skin met",,none,,"COSS910779",,adherent,,,,,2012-04-25,2016-04-04
50018,Ishikawa,,LCL-1497,"CLO_0037163",,,"European Collection of Authenticated Cell Cultures (ECACC) 99040201",Homo sapiens,uterus,,epithelial-like,,female,,,,"DOID:363, uterine cancer; DOID:1380, Endometrial cancer; DOID:2871, endometrial carcinoma; DOID:2870, endometrial adenocarcinoma",,,none,,,"STR-PCR Data (source: ECACC):
Amelogenin: X
CSF1PO: 11,12
D13S317: 9,12
D16S539: 9
D5S818: 10,11
D7S820: 9,10
THO1: 9,10
TPOX: 8
vWA: 14,17",adherent,"ECACC growth medium: MEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Foetal Bovine Serum (FBS). Subculture routine: Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 deg C; cells tend to pile up.",,,,2012-04-25,2016-08-24
50019,Ishikawa (Heraklio) 02 ER-,,LCL-1498,"CLO_0006967",,,European Collection of Authenticated Cell Cultures (ECACC) (discontinued),Homo sapiens,uterus,,epithelial,,female,,,,"DOID:363, uterine cancer; DOID:1380, Endometrial cancer; DOID:2871, endometrial carcinoma; DOID:2870, endometrial adenocarcinoma",,,none,,,,not specified,"From MGH/CMT as specified by cell provider: MEM + 10mM glutamine + 1% NEAA + 15% HI-FBS Split confluent cultures 1:8 i.e. seeding at 1-2 x 10,000 cells / cm2 using 0.25% trypsin or trypsin / EDTA; 5% CO2; 37C",,,,2012-04-25,2016-08-24
50020,IST-MEL1,,LCL-1234,"CLO_0006969",,,"Interlab Cell Line Collection (ICLC) HTL01006",Homo sapiens,skin,,epithelial-like,,male,,,,"DOID:1909, malignant melanoma",,,none,,"COSS907172",,adherent,"ICLC growth medium: RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:2-1:4 using trypsin/EDTA; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR",,,,2012-04-25,2016-04-04
50021,JHH-6,,LCL-1923,"CLO_0009993",,,"Japanese Collection of Research Bioresources Cell Bank JCRB1030",Homo sapiens,liver,,epithelial-like,,female,,,,"DOID:684, hepatocellular carcinoma",,,none,,"COSS1240159",,adherent,From MGH/CMT as specified by cell provider: William's E w/ 10% FCS,,,,2012-04-25,2016-07-12
50022,KATO III,,LCL-2003,"CLO_0007073",,,"ATCC HTB-103",Homo sapiens,stomach,,spherical,,male,,,,DOID:8025; gastric signet ring cell adenocarcinoma,,,none,,"COSS907276","DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 7,11
D13S317: 8,12
D16S539: 10,12
D5S818: 10,11
D7S820: 8,12
THO1: 7,9
TPOX: 11
vWA: 14,16","mixed, adherent and suspension","From MGH/CMT as specified by cell provider: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20% - OR - RPMI 1640 medium, 80%; fetal bovine serum, 20%",,,,2012-04-25,2016-04-04
50023,KMRC-20,,LCL-1755,"CLO_0009978",,,"Japanese Collection of Research Bioresources Cell Bank JCRB1071",Homo sapiens,kidney,,epithelial-like,,female,,,,"DOID:4450, renal cell adenocarcinoma",,,none,,"COSS1298169",,not specified,From MGH/CMT as specified by cell provider: Dulbecco's modified Eagle's medium with 10% fetal calf serum,,,,2012-04-25,2016-07-12
50024,KYSE-140,,LCL-1547,"CLO_0007128",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 348",Homo sapiens,esophagus,,,,male,,,,"DOID:3748, esophageal squamous cell carcinoma",,,none,,"COSS753573",,adherent,"From MGH/CMT as specified by cell provider: 90% RPMI 1640 + 10% FBS split confluent culture 1:5 to 1:10 every 2-4 days using trypsin/EDTA (cells may need five or more minutes to detach from the flask; prior to using trypsin, cells should be washed twice with PBS); seed out at ca. 2-3 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of ca. 20-30 x 10 6 cells/175 cm 2",,,,2012-04-25,2016-08-24
50025,KYSE-150,,LCL-1548,"CLO_0007129",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 375",Homo sapiens,esophagus,,epithelioid,,female,,,,"DOID:3748, esophageal squamous cell carcinoma",,,none,,"COSS907317",,adherent,From MGH/CMT as specified by cell provider: 49% RPMI 1640 + 49% Ham's F12 + 2% FBS split confluent culture 1:20 every 3-4 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/175 cm 2 at 37C with 5% CO2 cell harvest of ca. 1 x 105 cells/cm 2,,,,2012-04-25,2016-08-24
50026,KYSE-180,,LCL-1549,"CLO_0007130",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 379",Homo sapiens,esophagus,,epithelioid,,male,,,,"DOID:3748, esophageal squamous cell carcinoma",,,none,,"COSS907318",,adherent,From MGH/CMT as specified by cell provider: 90% RPMI 1640 + 10% FBS split confluent culture 1:5 to 1:10 every 3-5 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm 2 in 10 ml at 37C with 5% CO2 cell harvest of ca. 30 x 106 cells/175 cm 2,,,,2012-04-25,2016-08-24
50027,KYSE-450,,LCL-1550,"CLO_0007134",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 387",Homo sapiens,esophagus,,epithelioid,,male,,,,"DOID:3748, esophageal squamous cell carcinoma",,,none,,"COSS907320",,adherent,From MGH/CMT as specified by cell provider: 45% RPMI 1640 + 45% Ham's F12 + 10% FBS split confluent culture 1:8 to 1:10 every 3-4 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/25 cm 2 at 37 C with 5% CO2 cell harvest of ca. 5 x 106 cells/25 cm 2,,,,2012-04-25,2016-08-24
50028,LNZTA3WT4,LNZTA3p53WT4; WT4,LCL-1345,"CLO_0007371",,,"ATCC CRL-11543",Homo sapiens,brain,,,,unknown,,,,"DOID:3068, glioblastoma",,,none,,"COSS1240170","DNA Profile (STR, source: ATCC):
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 9,12
D5S818: 12
D7S820: 10,12
THO1: 9.3
TPOX: 8,9
vWA: 15,17",adherent,"From MGH/CMT as specified by cell provider: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 1 microgram/ml tetracycline, 90%; heat-inactivated fetal bovine serum, 10%.
Subculturing: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:10 is recommended",,,,2012-04-25,2016-04-04
50029,MCF7,,LCL-1460,"CLO_0007606",,,"ATCC HTB-22",Homo sapiens,breast,,epithelial,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS905946","DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15",adherent,"From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1 mM sodium pyruvate and supplemented with 0.01 mg/ml bovine insulin, 90%; fetal bovine serum, 10%.
Protocol: Remove culture medium to a centrifuge tube.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Transfer the cell suspension to the centrifuge tube with the medium and cells from step 1, and centrifuge at approximately 125 x g for 5 to 10 minutes. Discard the supernatant.
Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended",,,,2012-04-25,2016-04-04
50030,MDA-MB-435S,,LCL-1307,"CLO_0007638",,,"ATCC HTB-129",Homo sapiens,skin,,melanocyte,,female,,,,"DOID:1909, malignant melanoma (updated based on http://www.atcc.org/products/all/HTB-129.aspx)",,,none,,,"DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 13
D5S818: 12
D7S820: 8,10
THO1: 6,7
TPOX: 8,11
vWA: 16,18",adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with 0.01 mg/ml insulin, 90%; fetal bovine serum, 10%.
Protocol: Remove medium, add fresh 0.25%trypsin - 0.053 mM EDTA, rinse and remove. Place flask at room temperature (or incubated at 37C) for approximately 10 minutes or until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended",,,,2012-04-25,2016-07-12
50031,MT-3,,LCL-1473,"CLO_0007890",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 403",Homo sapiens,breast,,epithelial,,female (probably),,,,"DOID:3459, breast carcinoma",,,none,,,,adherent as colonies,From MGH/CMT as specified by cell provider: 90% RPMI 1640 + 10% FBS + 2 mM L-glutamine + 1 mM sodium pyruvate split confluent culture 1:10 every 4-5 days using trypsin/EDTA; seed out at ca. 1 x 10 6 cells/25 cm 2 at 37C with 5% CO2 cell harvest of ca. 0.4 x 106 cells/cm 2,,,,2012-04-25,2016-08-24
50032,NCI-H1648,H1648,LCL-1632,"CLO_0008006",,,"ATCC CRL-5882",Homo sapiens,lung,,,,male,,,,"DOID:3910, lung adenocarcinoma",NSCLC adenocarcinoma,,none,,"COSS687799","DNA Profile (STR, source: ATCC):
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 10,11
THO1: 7,9.3
TPOX: 8,11
vWA: 14,17",adherent,"From MGH/CMT as specified by cell provider: ACL-4 medium (serum-free). ACL-4 medium is formulated at the ATCC as follows:
A 1:1 mixture of Ham's F12 medium with 2.5 mM L-glutamine and Dulbecco's Modified Eagle's Medium adjusted to contain 1.2 g/L sodium bicarbonate (available as ATCC 30-2006) with additional supplements at the concentrations below:
0.02 mg/ml insulin
0.01 mg/ml transferrin
25 nM sodium selenite
50 nM Hydrocortisone
1 ng/ml Epidermal Growth Factor (do not filter)
0.01 mM ethanolamine
0.01 mM phosphorylethanolamine
100 pM triiodothyronine
0.5% (w/v) bovine serum albumin
10 mM HEPES
0.5 mM sodium pyruvate
2mM L-glutamine",,,,2012-04-25,2016-04-04
50033,NCI-H1651,H1651,LCL-1633,"CLO_0008008",,,"ATCC CRL-5884",Homo sapiens,lung,,epithelial,,male,,,,"DOID:3910, lung adenocarcinoma",NSCLC adenocarcinoma,,none,,"COSS910900","DNA Profile (STR, source: ATCC): Amelogenin: X
CSF1PO: 10,13
D13S317: 11
D16S539: 11
D5S818: 12,14
D7S820: 8
THO1: 8
TPOX: 11
vWA: 18",adherent,"From MGH/CMT as specified by cell provider: ACL-4 medium supplemented with 10% FBS. ACL-4 medium is formulated at the ATCC as follows:
A 1:1 mixture of Ham's F12 medium with 2.5 mM L-glutamine and Dulbecco's Modified Eagle's Medium adjusted to contain 1.2 g/L sodium bicarbonate (available as ATCC 30-2006) with additional supplements at the concentrations below:
0.02 mg/ml insulin
0.01 mg/ml transferrin
25 nM sodium selenite
50 nM Hydrocortisone
1 ng/ml Epidermal Growth Factor (do not filter)
0.01 mM ethanolamine
0.01 mM phosphorylethanolamine
100 pM triiodothyronine
0.5% (w/v) bovine serum albumin
10 mM HEPES
0.5 mM sodium pyruvate
2mM L-glutamine",,,,2012-04-25,2016-04-04
50034,NCI-H1703,H1703,LCL-1582,"CLO_0008014",,,"ATCC CRL-5889",Homo sapiens,lung,,epithelial,,male,,,,"DOID:3907, lung squamous cell carcinoma",NSCLC squamous cell carcinoma,,none,,"COSS908474","DNA Profile (STR, source: ATCC):
Amelogenin: X,Y
CSF1PO: 12
D13S317: 10
D16S539: 10,12
D5S818: 11
D7S820: 10,12
THO1: 7
TPOX: 8,11
vWA: 16,17",adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%",,,,2012-04-25,2016-04-04
50035,NCI-H1915,H1915,LCL-1598,"CLO_0008029",,,"ATCC CRL-5904",Homo sapiens,lung,,large cell,,female,,,,"DOID:3908, non-small cell lung carcinoma",NSCLC carcinoma,,none,,"COSS1240184",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium, 90%; fetal bovine serum, 10%",,,,2012-04-25,2016-04-04
50036,NCI-H2023,H2023,LCL-1599,"CLO_0008039",,,"ATCC CRL-5912",Homo sapiens,lung,,,,male,,,,"DOID:3908, non-small cell lung carcinoma",NSCLC carcinoma,,none,,"COSS1240187","DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 11,12
D5S818: 12
D7S820: 11
THO1: 7,9
TPOX: 8
vWA: 18",not specified,"From MGH/CMT as specified by cell provider: HITES medium supplemented with 5% fetal bovine serum. HITES medium with 5% fetal bovine serum is formulated at the ATCC as follows:
Dulbecco's medium : Ham's F12, 50:50 mix (ATCC 30-2006)
Insulin 0.005 mg/ml
Transferrin 0.01 mg/ml
Sodium selenite 30 nM
Hydrocortisone 10 nM
beta-estradiol 10 nM
HEPES 10 mM
L-glutamine 2 mM (in addition to that in the base medium)
fetal bovine serum 5%",,,,2012-04-25,2016-07-12
50037,NCI-H810,H810,LCL-1670,"CLO_0008117",,,"ATCC CRL-5816",Homo sapiens,lung,,epithelial,,male,,,,"DOID:3910, lung adenocarcinoma, DOID:4556, large cell carcinoma",NSCLC giant cell carcinoma,,none,,"COSS925341","DNA Profile (STR, source: ATCC):
Amelogenin: X,Y
CSF1PO: 10
D13S317: 12,13
D16S539: 9
D5S818: 11
D7S820: 10
THO1: 7,9
TPOX: 10,11
vWA: 15,16",adherent,"From MGH/CMT as specified by cell provider: HITES medium supplemented with 5% fetal bovine serum. HITES medium with 5% fetal bovine serum is formulated at the ATCC as follows:
Dulbecco's medium : Ham's F12, 50:50 mix (ATCC 30-2006)
Insulin 0.005 mg/ml
Transferrin 0.01 mg/ml
Sodium selenite 30 nM
Hydrocortisone 10 nM
beta-estradiol 10 nM
HEPES 10 mM
L-glutamine 2 mM (in addition to that in the base medium)
fetal bovine serum 5%",,,,2012-04-25,2016-04-04
50038,PC-9,,LCL-1630,"CLO_0050067",,,"European Collection of Authenticated Cell Cultures (ECACC) 90071810",Homo sapiens,lung,,,,not specified,,,,DOID:3908; non-small cell lung cancer (disorder),,,none,,,,adherent,From MGH/CMT as specified by cell provider: RPMI 1640 + 10% FBS + PS,,,,2012-04-25,2016-08-24
50039,PE/CA-PJ15,,LCL-1212,"CLO_0009951",,,"European Collection of Authenticated Cell Cultures (ECACC) 96121230",Homo sapiens,head & neck,,epithelial-like,,unknown,,,,"DOID:1749, DOID:8649, tongue squamous carcinoma",,,none,,"COSS1240207",,adherent,"ECACC growth medium: IDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Subculture routine: split sub-confluent cultures (70-80%) 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 deg C. Note: cells may take up to 7 days to become confluent, a medium change during this period may be required.",,,,2012-04-25,2016-08-24
50040,PL4,,LCL-1098,"CLO_0037112",,,Cyril Benes (Massachusetts General Hospital),Homo sapiens,pancreas,,,,not specified,,,,"DOID:4905, pancreatic carcinoma",,,none,,"COSS1298533",,adherent,,,,,2012-04-25,2016-07-12
50041,SJCRH30,RC13; RMS 13; SJRH30,LCL-1408,"CLO_0037076",,,"ATCC CRL-2061",Homo sapiens,muscle,,fibroblast,,male,,,,"DOID:3247, rhabdomyosarcoma",,,none,,"COSS909716","DNA Profile (STR, source: ATCC):
D5S818: 12, 13
D13S317: 11
D7S820: 10
D16S539: 12
THO1: 9, 9.3
TPOX: 8, 11
vWA: 17, 18
CSF1PO: 10, 11
Amelogenin: XY",adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%",,,,2012-04-25,2016-07-12
50042,SK-LMS-1,,LCL-1286,"CLO_0009038",,,"ATCC HTB-88",Homo sapiens,uterus,,,,female,,,,"DOID:1967, Leiomyosarcoma",sarcoma; leiomyosarcoma uterus,,none,,"COSS909720",,adherent,,,,,2012-04-25,2016-04-04
50043,SK-MES,SK-MES-1; SK MES 1,LCL-1583,"CLO_0009049",,,"ATCC HTB-58",Homo sapiens,lung,,epithelial,,male,,,,"DOID:3907, lung squamous cell carcinoma",NSCLC squamous cell carcinoma,,none,,"COSS909728","STR-PCR Data (source: ECACC):
Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 13
D5S818: 11
D7S820: 8
THO1: 6,9.3
TPOX: 8
vWA: 14",adherent,"From MGH/CMT as specified by cell provider: Ham's F10 : DMEM (1:1) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 i.e. seeding at 4-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.",,,,2012-04-25,2016-04-04
50044,SK-OV-3,,LCL-1517,"CLO_0009063",,,"ATCC HTB-77",Homo sapiens,ovary,,epithelial,,female,,,,"DOID:3713, ovarian adenocarcinoma",,,none,,"COSS905959","STR-PCR Data (source: ECACC):
Amelogenin: X
CSF1PO: 11
D13S317: 8,11
D16S539: 12
D5S818: 11
D7S820: 13,14
THO1: 9,9.3
TPOX: 8,11
vWA: 17,18",adherent,"From MGH/CMT as specified by cell provider: McCoy's 5a + 2mM Glutamine + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 3-6x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.",,,,2012-04-25,2016-07-12
50045,SNB75,SNB-75,LCL-1346,"CLO_0009905",,,"DTP, DCTD Tumor Repository, National Cancer Institute",Homo sapiens,brain,,,,not specified,,,,"DOID:3068, glioblastoma",,,none,,"COSS905982",,adherent,,,,,2012-04-25,2016-07-12
50046,SW527,SW 527; SW-527,LCL-1474,"CLO_0009219",,,"ATCC CRL-7940",Homo sapiens,breast,,epithelial,,female,,,,"DOID:3459, breast carcinoma",,,none,,,,adherent,"From MGH/CMT as specified by cell provider: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; fetal bovine serum, 10%",,,,2012-04-25,2016-07-12
50047,SW620,SW-620,LCL-1157,"CLO_0009221",,,"ATCC CCL-227",Homo sapiens,intestine,,epithelial,,male,,,,"DOID:1520, colon carcinoma","colon adenocarcinoma, Dukes' type C",,none,,"COSS905962","DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 13,14
D13S317: 12
D16S539: 9,13
D5S818: 13
D7S820: 8,9
THO1: 8
TPOX: 11
vWA: 16",adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%",,,,2012-04-25,2016-04-04
50048,T24,T-24,LCL-1709,"CLO_0009245",,,"ATCC HTB-4",Homo sapiens,urinary bladder,,epithelial,,female,,,,"DOID:4007, bladder carcinoma; DOID:4006, bladder transitional cell carcinoma",transitional cell carcinoma; urothelial bladder carcinoma,,none,,"COSS724812","DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 10,12
D13S317: 12
D16S539: 9
D5S818: 10,12
D7S820: 10,11
THO1: 6
TPOX: 8,11
vWA: 17",adherent,"From MGH/CMT as specified by cell provider: McCoy's 5a medium (modified) with 1.5 mM L-glutamine adjusted to contain 2.2 g/L sodium bicarbonate, 90%; fetal bovine serum, 10%.
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended",,,,2012-04-25,2016-07-12
50049,WiDr,,LCL-1169,"CLO_0009600",,,"ATCC CCL-218",Homo sapiens,intestine,,epithelial,,female,,,,"DOID:1520, DOID:234, colon adenocarcinoma",,,none,,,"STR-PCR Data (source: ECACC):
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19",adherent,"From MGH/CMT as specified by cell provider: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.",,,,2012-04-25,2016-07-12
50056,HME1,hTERT-HME1,LCL-2083,"CLO_0004291",,,"ATCC CRL-4010",Homo sapiens,breast,,epithelial,,female,,,,not diseased,,"Human mammary epithelium, HME1 cell line, was derived from a 53-year-old patient undergoing reduction mammoplasty surgery (no history of breast cancer).
The HME1 cells were immortalized by infection with the retrovirus pBabepuro+hTERT vector and cultured in complete growth medium containing puromycin until stable clones were selected.",pBabepuro+hTERT integration,,,"DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 11,12
D5S818: 11
D7S820: 7,12
THO1: 7,8
TPOX: 10,12
vWA: 15,16",adherent,"ATCC complete growth medium: The base medium for this cell line (MEBM) along with all the additives can be obtained from Lonza/Clonetics Corporation as a kit: MEGM, Kit Catalog No. CC-3150. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with kit. Note: Do not filter complete medium
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0 deg C
Subculturing protocol:
Remove and discard culture medium.
Add 2.0 to 3.0 ml of 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to the flask and observe cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
To remove trypsin-EDTA solution, add 2.0 to 3.0 ml of 0.1% SoybeanTrypsin Inhibitor solution and aspirate cells by gently pipetting.
Transfer cell suspension to a 15 mL centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes.
Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 7 X 10(3) to 9 X 10(3) viable cells/sq. cm is recommended. Subculture cultures when they reach a cell concentration between 4 X 10(4) and 6 X 10(4) cells/sq. cm.
Incubate cultures at 37C.
Interval: weekly
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended.
Medium Renewal: Every 2 to 3 days",,,,2012-05-01,2016-07-12
50057,SK-BR-3,SKBr3,LCL-1475,"CLO_0009034",,,"ATCC HTB-30",Homo sapiens,breast,,epithelial,,female,,,,"DOID:3459, breast carcinoma",,,none,,,"DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 12
D13S317: 11,12
D16S539: 9
D5S818: 9,12
D7S820: 9,12
THO1: 8,9
TPOX: 8,11
vWA: 17",adherent,"ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0 deg C
Subculturing protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin, 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37 deg C.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week",,,,2012-05-01,2016-07-12
50058,MDA-MB-231,MDAMB231; MDA-MB231,LCL-1461,"CLO_0007634",,,"ATCC HTB-26",Homo sapiens,breast,,epithelial,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS905960","DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 12,13
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 8,9
THO1: 7,9.3
TPOX: 8,9
vWA: 15,18",adherent,"ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 37.0 deg C
Subculturing protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37 deg C without CO2.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week",,,,2012-05-01,2016-07-12
50060,A-375,A375,LCL-1235,"CLO_0001582",,,"ATCC CRL-1619",Homo sapiens,skin,,epithelial,,female,,,,"DOID:1909, malignant melanoma",,,none,,"COSS906793","DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 8
TPOX: 8,10
vWA: 16,17",adherent,"ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0 deg C
Subculturing protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37 deg C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days",,,,2012-05-01,2016-07-12
50061,HeLa,,LCL-1512,"CLO_0003684",,,"ATCC CRM-CCL-2",Homo sapiens,cervix,,epithelial,,female,,,,"DOID:3702, cervical adenocarcinoma",,,none,,"COSS1298134","DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18",adherent,"ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0 deg C
Subculturing protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37 deg C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week",,,,2012-05-01,2016-07-12
50084,A549,,LCL-1601,"CLO_0001601",,,"ATCC CCL-185",Homo sapiens,lung,,,,male,,,,"DOID:3908, non-small cell lung carcinoma",NSCLC carcinoma,,none,,"COSS905949",,adherent,"From MGH/CMT as specified by cell provider: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 90%; fetal bovine serum, 10%",,,,2012-07-31,2016-04-04
50091,AU565,AU-565,LCL-1462,"CLO_0001769",,,"ATCC CRL-2351",Homo sapiens,breast,,,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS910704",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Subculturing: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended",,,,2012-07-31,2016-04-04
50105,BT-20,,LCL-1476,"CLO_0002041",,,"ATCC HTB-19",Homo sapiens,breast,,,,female,,,,"DOID:3459, breast carcinoma",breast ductal carcinoma,,none,,"COSS906801",,adherent,"From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended",,,,2012-07-31,2016-04-04
50106,BT-474,,LCL-1308,"CLO_0002042",,,"ATCC HTB-20",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS946359",,adherent,"From MGH/CMT as specified by cell provider: Hybri-Care Medium ATCC 46-X, 90%; fetal bovine serum, 10%.
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended",,,,2012-07-31,2016-04-04
50107,BT-483,,LCL-1309,"CLO_0002043",,,"ATCC HTB-121",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS949093",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.01 mg/ml bovine insulin, 80%; fetal bovine serum, 20%.
Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 3 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 is recommended",,,,2012-07-31,2016-04-04
50108,BT-549,,LCL-1310,"CLO_0002044",,,"ATCC HTB-122",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS905951",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 0.023 IU/ml insulin and 10% fetal bovine serum.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended",,,,2012-07-31,2016-04-04
50112,C32,,LCL-1238,"CLO_0002113",,,"ATCC CRL-1585",Homo sapiens,skin,,,,male,,,,"DOID:1909, malignant melanoma",,,none,,"COSS906830",,adherent,"From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%",,,,2012-07-31,2016-04-04
50131,CAMA-1,,LCL-1466,"CLO_0002194",,,"ATCC HTB-21",Homo sapiens,breast,,,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS946382",,adherent,"From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:4 is recommended",,,,2012-07-31,2016-04-04
50149,COLO 858,,LCL-1242,"CLO_0002569",,,"European Collection of Authenticated Cell Cultures (ECACC) 93052613",Homo sapiens,skin,,,,male,,,,"DOID:1909, malignant melanoma",,,none,,,,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37C; 5% CO2.",,,,2012-07-31,2016-08-24
50205,HCC1143,,LCL-1329,"CLO_0003630",,,"ATCC CRL-2321",Homo sapiens,breast,,,,female,,,,"DOID:3008, primary ductal carcinoma",breast ductal carcinoma,,none,,"COSS749710",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol: Remove medium, rinse with 0.25% trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended",,,,2012-07-31,2016-04-04
50206,HCC1395,HCC-1395,LCL-1330,"CLO_0003634",,,"ATCC CRL-2324",Homo sapiens,breast,,,,female,,,,"DOID:3008, primary ductal carcinoma",breast ductal carcinoma,,none,,"COSS749712",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 is recommended",,,,2012-07-31,2016-04-04
50207,HCC1419,HCC-1419,LCL-1314,"CLO_0003636",,,"ATCC CRL-2326",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS907045",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Growth Conditions: The line grows as large epithelial attached cells in island-like formation.
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended",,,,2012-07-31,2016-04-04
50208,HCC1428,HCC-1428,LCL-1467,"CLO_0003637",,,"ATCC CRL-2327",Homo sapiens,breast,,,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS1290905",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:8 is recommended",,,,2012-07-31,2016-04-04
50210,HCC1569,HCC-1569,LCL-1480,"CLO_0003640",,,"ATCC CRL-2330",Homo sapiens,breast,,,,female,,,,"DOID:3459, breast carcinoma",breast carcinoma; metastatic carcinoma,,none,,"COSS907046",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Subculturing: Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended",,,,2012-07-31,2016-04-04
50211,HCC1806,HCC-1806,LCL-1960,"CLO_0003644",,,"ATCC CRL-2335",Homo sapiens,breast,,,,female,,,,"DOID:7459, primary acantholytic squamous cell carcino",breast ductal carcinoma,,none,,"COSS907047",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Subculturing: Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended",,,,2012-07-31,2016-04-04
50212,HCC1937,HCC-1937,LCL-1331,"CLO_0003645",,,"ATCC CRL-2336",Homo sapiens,breast,,,,female,,,,"DOID:3008, primary ductal carcinoma",breast ductal carcinoma,,none,,"COSS749714",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol: Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended",,,,2012-07-31,2016-04-04
50213,HCC1954,HCC-1954,LCL-1332,"CLO_0003647",,,"ATCC CRL-2338",Homo sapiens,breast,,,,female,,,,"DOID:3008, primary ductal carcinoma",breast ductal carcinoma,,none,,"COSS749709",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:8 is recommended",,,,2012-07-31,2016-04-04
50214,HCC202,HCC-202,LCL-1333,"CLO_0003649",,,"ATCC CRL-2316",Homo sapiens,breast,,,,female,,,,"DOID:3008, primary ductal carcinoma",breast ductal carcinoma,,none,,"COSS1290906",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Subculturing: Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended",,,,2012-07-31,2016-04-04
50216,HCC38,HCC-38,LCL-1334,"CLO_0003655",,,"ATCC CRL-2314",Homo sapiens,breast,,,,female,,,,"DOID:3008, primary ductal carcinoma",breast ductal carcinoma,,none,,"COSS749717",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended",,,,2012-07-31,2016-04-04
50219,HCC70,HCC-70,LCL-1335,"CLO_0003658",,,"ATCC CRL-2315",Homo sapiens,breast,,,,female,,,,"DOID:3008, primary ductal carcinoma",breast ductal carcinoma,,none,,"COSS907048",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended",,,,2012-07-31,2016-04-04
50238,Hs 578T,Hs-578-T,LCL-1315,"CLO_0004009",,,"ATCC HTB-126",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS905957",,adherent,"From MGH/CMT as specified by cell provider: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.01 mg/ml bovine insulin and 10% fetal bovine serum.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended",,,,2012-07-31,2016-07-12
50324,MB 157,,LCL-1484,"CLO_0007544",,,"ATCC CRL-7721",Homo sapiens,breast,,,,female,,,,"DOID:3459, breast carcinoma",,,none,,,,adherent,"From MGH/CMT as specified by cell provider: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 90%; fetal bovine serum, 10%.
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: 1:2 to 1:3",,,,2012-07-31,2016-07-12
50327,MDA-MB-134-VI,,LCL-1316,"CLO_0007631",,,"ATCC HTB-23",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,,,lightly adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 80%; fetal bovine serum, 20%.
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended",,,,2012-07-31,2016-07-12
50328,MDA-MB-157,,LCL-1916,"CLO_0007632",,,"ATCC HTB-24",Homo sapiens,breast,,,,female,,,,"DOID:5605, breast medullary carcinoma",,,none,,"COSS925338",,adherent,"From MGH/CMT as specified by cell provider: LLeibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C without CO2.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended",,,,2012-07-31,2016-04-04
50329,MDA-MB-175-VII,,LCL-1317,"CLO_0007633",,,"ATCC HTB-25",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS908120",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%.
Protocol:
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended",,,,2012-07-31,2016-04-04
50331,MDA-MB-361,,LCL-1468,"CLO_0007636",,,"ATCC HTB-27",Homo sapiens,breast,,,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS908121",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 80%; fetal bovine serum, 20%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended",,,,2012-07-31,2016-04-04
50332,MDA-MB-415,,LCL-1469,"CLO_0007637",,,"ATCC HTB-128",Homo sapiens,breast,,,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS924240",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2mM L-glutamine supplemented with 10 mcg/ml insulin and 10 mcg/ml glutathione, 85%; fetal bovine serum, 15%.
Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation ratio: A subcultivation ratio of 1:2 is recommended",,,,2012-07-31,2016-04-04
50333,MDA-MB-436,,LCL-1470,"CLO_0007639",,,"ATCC HTB-130",Homo sapiens,breast,,,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS1240172",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 10 mcg/ml insulin, 16 mcg/ml glutathione, 90%; fetal bovine serum, 10%.
Subculturing: Subcultures are prepared by scraping. Remove spent medium, add 3 to 5 ml of fresh medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation ratio: A subcultivation ratio of 1:2 is recommended",,,,2012-07-31,2016-04-04
50334,MDA-MB-453,,LCL-1485,"CLO_0007640",,,"ATCC HTB-131",Homo sapiens,breast,,,,female,,,,"DOID:3459, breast carcinoma",breast carcinoma; metastatic carcinoma,,none,,"COSS908122",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C without CO2.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended",,,,2012-07-31,2016-04-04
50335,MDA-MB-468,,LCL-1471,"CLO_0007641",,,"ATCC HTB-132",Homo sapiens,breast,,,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS908123",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended",,,,2012-07-31,2016-04-04
50454,PC-3,PC3,LCL-1299,"CLO_0008395",,,"ATCC CRL-1435",Homo sapiens,prostate,,,,male,,,,"DOID:2526, Prostate adenocarcinoma",prostate adenocarcinoma; bone metastasis,,none,,"COSS905934",,adherent,,,,,2012-07-31,2016-04-04
50475,RVH-421,,LCL-1255,"CLO_0008902",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 127",Homo sapiens,skin,,,,male,,,,"DOID:1909, malignant melanoma",,,none,,"COSS909706",,adherent,From MGH/CMT as specified by cell provider: 90% RPMI 1640 (or Leibovitz's L-15) + 10% FBS split confluent culture up to 1:10 one to two times per week using trypsin/EDTA; seed out 1-2 x 106 cells/175 cm 2 at 37C with 5% CO2 about 15 x 106 cells/175 cm 2,,,,2012-07-31,2016-08-24
50541,T47D,T-47D,LCL-1486,"CLO_0009251",,,"ATCC HTB-133",Homo sapiens,breast,,,,female,,,,"DOID:3459, breast carcinoma",,,none,,"COSS905945",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 or DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 37C.",,,,2012-07-31,2016-07-12
50556,UACC-812,,LCL-1319,"CLO_0009467",,,"ATCC CRL-1897",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS910910",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with 20 ng/ml human EGF and 20% fetal bovine serum.
Growth Conditions: The cells grow very slowly, and growth is enhanced by using 20% fetal bovine serum and adding epidermal growth factor (20 ng/ml) to the medium.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended",,,,2012-07-31,2016-04-04
50557,UACC-893,,LCL-1320,"CLO_0009468",,,"ATCC CRL-1902",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS909778",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended",,,,2012-07-31,2016-04-04
50567,WM-115,,LCL-1260,"CLO_0009613",,,"ATCC CRL-1675",Homo sapiens,skin,,,,female,,,,"DOID:1909, malignant melanoma",,,none,,"COSS909784",,adherent,"From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.03% EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 34 to 35°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 34 to 35°C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended",,,,2012-07-31,2016-04-04
50568,WM1552C,,LCL-1261,"CLO_0009614",,,"ATCC CRL-2808",Homo sapiens,skin,,,,male,,,,"DOID:1909, malignant melanoma",,,none,,"COSS1299078",,adherent,"From MGH/CMT as specified by cell provider: 2% Tumor Medium (Tu2%) containing a 4:1 mixture of MCDB 153 medium with 1.5 g/L sodium bicarbonate and Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with 0.005 mg/ml bovine insulin, 1.68 mM CaCl2, and 2% fetal bovine serum.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
An inoculum of 5 X 10(3) to 7 X 10(3) viable cells/cm2 is recommended. .
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation of 1:3 to 1:4 is recommended",,,,2012-07-31,2016-04-04
50574,ZR-75-1,,LCL-1321,"CLO_0009727",,,"ATCC CRL-1500",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,,,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended",,,,2012-07-31,2016-07-12
50575,ZR-75-30,,LCL-1322,"CLO_0009728",,,"ATCC CRL-1504",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS909907",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended",,,,2012-07-31,2016-04-04
50576,184B5,,LCL-2081,"CLO_0001138",,,"ATCC CRL-8799",Homo sapiens,breast,,epithelial,,female,,,,not diseased,,"Cells derived from the tissue were exposed to benzo(a)pyrene, and a transformed line was established. The 184B5 cell line was established from normal mammary tissue obtained from a normal reduction mammoplasty.",none,,,,adherent,,,,,2012-11-02,2016-04-04
50577,DU4475,,LCL-1323,"CLO_0002842",,,"ATCC HTB-123",Homo sapiens,breast,,epithelial,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS906844",,suspension,,,,,2012-11-02,2016-04-04
50578,HCC1187,,LCL-1324,"CLO_0003632",,,"ATCC CRL-2322",Homo sapiens,breast,duct,epithelial,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS749711",,"mixed, adherent and suspension",,,,,2012-11-02,2016-07-12
50579,HCC1500,,LCL-1325,"CLO_0003639",,,"ATCC CRL-2329",Homo sapiens,breast,duct,epithelial,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS1303900",,adherent,,,,,2012-11-02,2016-04-04
50580,HCC1599,,LCL-1326,"CLO_0003641",,,"ATCC CRL-2331",Homo sapiens,breast,duct,epithelial,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS749713",,suspension,,,,,2012-11-02,2016-04-04
50581,HCC2157,,LCL-1327,"CLO_0003650",,,"ATCC CRL-2340",Homo sapiens,breast,duct,epithelial,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS749715",,suspension,,,,,2012-11-02,2016-04-04
50582,HCC2218,,LCL-1328,"CLO_0003652",,,"ATCC CRL-2343",Homo sapiens,breast,duct,epithelial,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS749716",,suspension,,,,,2012-11-02,2016-04-04
50583,MCF 10A,,LCL-2085,"CLO_0007599",,,"ATCC CRL-10317",Homo sapiens,breast,,epithelial,,female,,,,fibrocystic disease,non-tumorigenic,,none,,,,adherent,,,,,2012-11-02,2016-04-04
50584,MCF 10F,,LCL-2102,"CLO_0007600",,,"ATCC CRL-10318",Homo sapiens,breast,,epithelial,,female,,,,fibrocystic disease,non-tumorigenic,,none,,,,adherent,,,,,2012-11-02,2016-04-04
50811,Ramos (RA 1),,LCL-1097,"CLO_0008739",,,"ATCC CRL-1596",Homo sapiens,blood,,,,male,,,,Burkitt's lymphoma,,,none,,,,suspension,,,,,2012-12-17,2016-07-12
50861,THP-1,,LCL-1072,"CLO_0009348",,,"ATCC TIB-202",Homo sapiens,blood,,,,male,,,,AML,,,none,,"COSS909771",,suspension,,,,,2012-12-17,2016-07-12
50868,U-87 MG,U-87-MG,LCL-1364,"CLO_0009464",,,"ATCC HTB-14",Homo sapiens,brain,,,,female,,,,"DOID:3068, glioblastoma",,,none,,"COSS687590",,adherent,,,,,2012-12-17,2016-07-12
50869,U-937,,LCL-1125,"CLO_0009465",,,"ATCC CRL-1593.2",Homo sapiens,lymphatic system,,,,male,,,,"DOID:0050745, diffuse large B-cell lymphoma, DOID:0060060, non-Hodgkin's lymphoma; DOID:707, B-cell lymphocytic neoplasm",histiocytic lymphoma,,none,,,,suspension,,,,,2012-12-17,2016-07-12
50988,LOXIMVI,LOX IMVI,LCL-1276,"CLO_0037130",,,"DTP, DCTD Tumor Repository, National Cancer Institute",Homo sapiens,skin,,,,male,,,,"DOID:1909, malignant melanoma",malignant melanoma; metastatic,,none,,"COSS905974",,semi-adherent,,,,,2012-07-31,2016-07-12
50999,MMAC-SF,,LCL-1277,"CLO_0037131",,,"Cell Bank, RIKEN BioResource Center RCB1200",Homo sapiens,skin,,,,na,,,,"DOID:1909, malignant melanoma",malignant melanoma; ascites,,none,,"COSS925339",,adherent,,,,,2012-07-31,2016-07-12
51006,MZ7-mel,,LCL-1279,"CLO_0037133",,,Thomas Woelfel (Johannes Gutenberg University Mainz),Homo sapiens,skin,,,,female,,,,"DOID:1909, malignant melanoma",,,none,,"COSS753596",,adherent,,,,,2012-07-31,2016-07-12
51081,SUM1315MO2,SUM-1315MO2,LCL-2066,"CLO_0009916",,,University of Michigan (http://www.cancer.med.umich.edu/breast_cell/Production/index.html),Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,"PMID 10604729",,,2013-07-30,2016-07-12
51082,SUM149PT,SUM-149PT,LCL-2067,"CLO_0009917",,,"Asterand SUM-149PT",Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,"PMID 10604729",,,2013-07-30,2016-07-12
51083,SUM159PT,SUM-159PT,LCL-2068,"CLO_0009918",,,"Asterand SUM-159PT",Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,"PMID 10604729",,,2013-07-30,2016-07-12
51084,SUM185PE,SUM-185PE,LCL-2069,"CLO_0009919",,,"Asterand SUM-185PE",Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,"PMID 10604729",,,2013-07-30,2016-07-12
51085,SUM225CWN,SUM-225CWN,LCL-2070,"CLO_0009920",,,"Asterand SUM-225CWN",Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,"PMID 10604729",,,2013-07-30,2016-07-12
51086,SUM229PE,SUM-229PE,LCL-2065,"CLO_0009921",,,"Asterand SUM-229PE",Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,"PMID 10604729",,,2013-07-30,2016-07-12
51087,SUM52PE,SUM-52E,LCL-2071,"CLO_0037189",,,"Asterand SUM-52PE",Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,"PMID 10604729",,,2013-07-30,2016-07-12
51088,184A1,,LCL-2080,"CLO_0001137",,,"ATCC CRL-8798",Homo sapiens,breast,,,,,,,,,,,none,,,,adherent,,,,,2013-07-30,2016-07-12
51089,MCF12A,MCF-12A,LCL-2082,"CLO_0007603",,,"ATCC CRL-10782",Homo sapiens,breast,,,,,,,,,,,none,,,,adherent,,,,,2013-07-30,2016-07-12
51090,MX1,MX-1,LCL-2072,"CLO_0009915",,,"DTP, DCTD Tumor Repository, National Cancer Institute",Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,,,,2013-07-30,2016-07-12
51091,T47DKBLUC,T47D-KBluc,LCL-2064,"CLO_0037191",,,"ATCC CRL-2865",Homo sapiens,breast,,,,,,,,,,The parent cell line was transfected with pGL2.TATA.Inr.luc.neo which contains three estrogen responsive elements upstream of a luc reporter gene. The cells were selected for responsiveness to 17-beta-estradiol.,pGL2.TATA.Inr.luc.neo integration,,,,adherent,,,,,2013-07-30,2016-07-12
51092,600MPE,,LCL-2073,"CLO_0009908",,,H.S. Smith (California Pacific Medical Center),Homo sapiens,breast,,,,,,,,,,,,,,,,,"PMID 3470538",,,2013-07-30,2016-07-12
51093,HCC2185,,LCL-2074,"CLO_0009909",,,Adi Gazdar (University of Texas-Southwestern Medical Center),Homo sapiens,breast,,,,female,38,white,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,,,non-adherent,,"PMID 9833771",,,2013-07-30,2016-07-12
51094,HCC3153,,LCL-2075,"CLO_0009910",,,Adi Gazdar (University of Texas-Southwestern Medical Center),Homo sapiens,breast,,,,,,,,,,,,,,,,,,,,2013-07-30,2016-07-12
51095,LY2,LY 2,LCL-2076,"CLO_0009913",,,Mark Lippman (National Cancer Institute),Homo sapiens,breast,,,,,,,,,,"The cell line was selected by increasing the concentration of LY 117018, a potent antiestrogen that inhibits cell growth at concentrations as low as 10(-10) M, in the growth medium in a stepwise manner from 10(-8) to 10(-6) M as the cells become resistant.",none,,,,,,"PMID 4029083",,,2013-07-30,2016-07-12
51096,ZR75B,,LCL-2077,"CLO_0009924",,,Mark Lippman (National Cancer Institute),Homo sapiens,breast,,,,,,,,,,,,,,,,,"PMID 7439217",,,2013-07-30,2016-07-12
51097,BCWM.1,,LCL-2094,"CLO_0009833",,,Steven Treon (Dana Farber Cancer Center),Homo sapiens,blood,,,,female,,,,"DOID:0050747, lymphoplasmacytic lymphoma",B-cell lymphocytic neoplasm characterized by an uncontrolled increase of B-cells,,none,,,,,,"PMID 17761288",,,2014-06-26,2016-07-12
51098,MWCL.1,,,,,,Stephen M. Ansell (Mayo Clinic),Homo sapiens,blood,,,,male,73,,,"DOID:0050747, lymphoplasmacytic lymphoma",B-cell lymphocytic neoplasm characterized by an uncontrolled increase of B-cells,,none,,,,,,"PMID 21415268",,,2014-06-26,2016-07-12
51099,NGP,,LCL-2103,"CLO_0037091",,,"Creative Bioarray CSC-C2562",Homo sapiens,nervous system,,,,,,,,"DOID:769, neuroblastoma",,,,,,,,,"Thiele CJ. Neuroblastoma: In (Ed.) Masters, J. Human Cell Culture. Lancaster, UK: Kluwer Academic Publishers. 1998, Vol 1, p 21-53.",,,2014-06-26,2016-07-12
51100,K2,,LCL-2091,"CLO_0037127",,,Hensin Tsao (Masschusetts General Hospital),Homo sapiens,skin,,,,,,,,"DOID:1909, malignant melanoma",,,,,"COSS1298160",,,,,,,2015-01-10,2016-07-12
51101,SKMEL28,SK-MEL-28,LCL-2092,"CLO_0009043",,,"ATCC HTB-72",Homo sapiens,skin,,,,male,51,,,"DOID:1909, malignant melanoma",,,none,,"COSS905954",,adherent,,,,,2015-01-10,2016-07-12
51102,HeLa ICRP,,LCL-2096,"CLO_0003684",HeLa,HMSL50061-2,Jeremie Roux (Harvard Medical School),Homo sapiens,cervix,,epithelial,,,,,,"DOID:3702, cervical adenocarcinoma",,Modification type: stable retroviral transformation; integrated plasmid: IC-RP (2xIETD). Citation: IC-RP: Addgene Plasmid #24536.,IC-RP (2xIETD) integration,,,,adherent,,,,,2015-06-10,2016-07-12
51103,HeLa ICRP FLIP-L-mCherry,,LCL-2099,"CLO_0003684",HeLa ICRP,HMSL51102-1,Jeremie Roux (Harvard Medical School),Homo sapiens,cervix,,epithelial,,,,,,"DOID:3702, cervical adenocarcinoma",,Modification type: retroviral transformation with pQCXIP-FLIP-L-mCherry and maintenance as a heterogenous population; integrated plasmids: IC-RP (2xIETD); pQCXIP-FLIP-L-mCherry. Citations: IC-RP: Addgene Plasmid #24536; FLIP-L parental plasmid: Inna Lavrik (Otto von Guericke University).,IC-RP (2xIETD) and pQCXIP-FLIP-L-mCherry integration,,,,adherent,,,,,2015-06-10,2016-07-12
51104,HeLa ICRP FLIP-S-mCherry,,LCL-2100,"CLO_0003684",HeLa ICRP,HMSL51102-1,Jeremie Roux (Harvard Medical School),Homo sapiens,cervix,,epithelial,,,,,,"DOID:3702, cervical adenocarcinoma",,Modification type: retroviral transformation with pQCXIP-FLIP-S-mCherry and maintenance as a heterogenous population; integrated plasmids: IC-RP (2xIETD); pQCXIP-FLIP-S-mCherry. Citations: IC-RP: Addgene Plasmid #24536; FLIP-S parental plasmid: Inna Lavrik (Otto von Guericke University).,IC-RP (2xIETD) and pQCXIP-FLIP-S-mCherry integration,,,,adherent,,,,,2015-06-10,2016-07-12
51105,HeLa ICRP Bcl-2-mCherry,,LCL-2097,"CLO_0003684",HeLa ICRP,HMSL51102-1,Jeremie Roux (Harvard Medical School),Homo sapiens,cervix,,epithelial,,,,,,"DOID:3702, cervical adenocarcinoma",,"Modification type: retroviral transformation with pQCXIP-Bcl-2-mCherry and maintenance as a heterogenous population; integrated plasmids: IC-RP (2xIETD); pQCXIP-Bcl-2-mCherry. Citations: IC-RP: Addgene Plasmid #24536; pQCXIP-Bcl-2-mCherry: John Bachman (Peter Sorger group, Harvard Medical School).",IC-RP (2xIETD) and pQCXIP-Bcl-2-mCherry integration,,,,adherent,,,,,2015-06-10,2016-07-12
51106,HeLa ICRP Bcl-XL-mCherry,,LCL-2098,"CLO_0003684",HeLa ICRP,HMSL51102-1,Jeremie Roux (Harvard Medical School),Homo sapiens,cervix,,epithelial,,,,,,"DOID:3702, cervical adenocarcinoma",,"Modification type: retroviral transformation with pQCXIP-Bcl-XL-mCherry and maintenance as a heterogenous population; integrated plasmids: IC-RP (2xIETD); pQCXIP-Bcl-XL-mCherry. Citations: IC-RP: Addgene Plasmid #24536; pQCXIP-Bcl-XL-mCherry: John Bachman (Peter Sorger group, Harvard Medical School).",IC-RP (2xIETD) and pQCXIP-Bcl-XL-mCherry integration,,,,adherent,,,,,2015-06-10,2016-07-12
51107,21MT-1,,,,,,Ruth Sager (Dana-Farber Cancer Institute),Homo sapiens,breast,,epithelial,,female,36,,,"DOID:3005, invasive ductal carcinoma",breast ductal adenocarcinoma,established from the pleural effusion of lung metastases of a primary breast tumor,none,,,,adherent,,"PMID 2487147; PMID 1977518",,,2016-08-24,2016-08-24
51108,21NT,,,,,,Ruth Sager (Dana-Farber Cancer Institute),Homo sapiens,breast,,epithelial,,female,36,,,"DOID:3005, invasive ductal carcinoma",breast ductal adenocarcinoma,established from primary breast tumor,none,,,,adherent,,"PMID 1977518",,,2016-08-24,2016-08-24
51109,21PT,,,,,,Ruth Sager (Dana-Farber Cancer Institute),Homo sapiens,breast,,epithelial,,female,36,,,"DOID:3005, invasive ductal carcinoma",breast ductal adenocarcinoma,established from primary breast tumor,none,,,,adherent,,"PMID 1977518",,,2016-08-24,2016-08-24
51110,CAL-120,,,"CLO_0002179",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 459",Homo sapiens,breast,,,,female,43,,,"DOID:3458, breast adenocarcinoma",breast adenocarcinoma,From DSMZ: established from the pleural effusion of a metastatic breast adenocarcinoma in 1991,none,,,,adherent; From DSMZ: heterogenous population with epithelial-like and fibroblast-like adherent cells growing in monolayers,From DSMZ: Medium: 90% Dulbecco's MEM + 10% h.i. FBS. Subculture: split confluent culture 1:2 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/175 cm2. Incubation: at 37°C with 10% CO2. Doubling time: ca. 50-60 hours.,,,,2016-08-24,2016-08-24
51111,CAL-148,,,"CLO_0002181",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 460",Homo sapiens,breast,,epithelioid,,female,58,,,"DOID:3458, breast adenocarcinoma",breast adenocarcinoma,From DSMZ: established from the pleural effusion from a 58-year-old French woman with terminally invasive breast cancer (moderately differentiated ductal adenocarcinoma) after repeated cycles of chemotherapy (including adriamycin and taxol),none,,,,"mixed; From DSMZ: epitheloid cells growing as single cells or as clumps either in suspension or, if supplemented with EGF, in monolayers",From DSMZ: Medium: 80% Dulbecco's MEM + 20% h.i. FBS + 2 mM L-glutamine + EGF (1 µg/100 ml). Subculture: Split confluent culture 1:4 to 1:10 every 3-5 days by agitation. Seed out at ca. 0.2-0.3 x 106 cells/ml (suspension culture) or 0.4-0.8 x 105 cells/cm2 (adherent culture). Maintain at about 0.2-0.7 x 106 cells/ml or equivalent. Cell numbers may be difficult to ascertain due to growth in clumps. Cells grow faster in suspension without EGF. Incubation: at 37°C with 10% CO2. Doubling time: ca. 30-40 hours.,"PMID 7531416",,,2016-08-24,2017-05-02
51112,CAL-85-1,,,"CLO_0002190",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 440",Homo sapiens,breast,,epithelial,,female,35,,,"DOID:3458, breast adenocarcinoma",breast adenocarcinoma,From DSMZ: established from the relapsing invasive galactophoric breast adenocarcinoma resected from a 35-year-old woman during radiotherapy in 1990; cells were described as expressing the multidrug resistance (MDR) gene,none,,,,adherent; From DSMZ: adherent epithelial-like cells growing in monolayers,From DSMZ: Medium: 90% Dulbecco's MEM + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate. Subculture: Split confluent culture 1:2 to 1:3 every 3-4 days using trypsin/EDTA (incubation with trypsin/EDTA for about 10 min). Seed out at ca. 0.03-0.04 x 106 cells/cm2. Maintain at 0.04 x 106 cells/cm2. A significant amount of cell debris is always visible in the culture background which might be due to granula or other intracellular material. Incubation: at 37°C with 5% CO2. Doubling time: ca. 70 hours.,"Fischel, J. L., Formento, P., Lucas, C., Berlion, M., Bizzari, J. P., Milano, G. (1993) Le S9788 dans la réversion de la résistance MDR: Activité comparée à des composés de référence. Bull Cancer 80 : 409.",,,2016-08-24,2016-08-24
51113,EFM-19,,,"CLO_0002888",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 231",Homo sapiens,breast,,epithelioid,,female,50,Caucasian,,"DOID:3007, breast ductal carcinoma",breast ductal adenocarcinoma,"From DSMZ: established from the pleural effusion of a 50-year-old Caucasian woman with breast carcinoma (ductal type, histological grading II) in 1979",none,,,,"mixed; From DSMZ: epitheloid, growing in clusters at first, then as monolayer",From DSMZ: Medium: 90% RPMI 1640 + 10% h.i. FBS. Subculture: Split confluent culture 1:2 to 1:3 twice a week using trypsin/EDTA. Seed out at ca. 2 x 106 cells/25 cm2. Incubation: at 37°C with 5% CO2. Doubling time: about 64 hours.,"PMID 6325325",,,2016-08-24,2017-05-02
51114,EFM-192A,,,"CLO_0002889",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 258",Homo sapiens,breast,,epithelioid,,female,46,Caucasian,,"DOID:3458, breast adenocarcinoma",breast adenocarcinoma,From DSMZ: established from the pleural effusion of the left side from a 46-year-old Caucasian woman with metastasizing breast adenocarcinoma in 1985,none,ERBB2 amplification,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 258",,"adherent; From DSMZ: epitheloid, adherent growing as monolayer",From DSMZ: Medium: 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine. Subculture: Split confluent culture 1:2 to 1:3 once a week using trypsin/EDTA. Seed out at ca. 2 x 106 cells/25 cm2. Incubation: at 37°C with 5% CO2. Doubling time: about 9 days.,,,,2016-08-24,2017-05-02
51115,EFM-192B,,,"CLO_0002890",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 308",Homo sapiens,breast,,epithelioid,,female,46,Caucasian,,"DOID:3458, breast adenocarcinoma",breast adenocarcinoma,From DSMZ: established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma,none,,,,adherent; From DSMZ: epitheloid adherent cells growing as monolayers,From DSMZ: Medium: 80% RPMI 1640 + 20% h.i. FBS. Subculture: Subcultivation recommended before reaching confluence. Split 1:2 to 1:3 once a week using trypsin/EDTA. Seed out at ca. 0.5-1.0 x 105 cells/cm2. Incubation: at 37°C with 5% CO2. Doubling time: ca. 4-5 days.,,,,2016-08-24,2017-05-02
51116,EFM-192C,,,"CLO_0002891",,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 311",Homo sapiens,breast,,epithelioid,,female,46,Caucasian,,"DOID:3458, breast adenocarcinoma",breast adenocarcinoma,From DSMZ: established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma,none,ERBB2 amplification,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 311",,adherent; From DSMZ: epitheloid adherent cells growing as monolayers,From DSMZ: Medium: 80% RPMI 1640 + 20% h.i. FBS. Subculture: Optimal split ratio is 1:2 to 1:3 once a week using trypsin/EDTA. Seed out at ca. 5-8 x 104 cells/cm2 or 2 x 106 cells/25 cm2 flask. Incubation: at 37°C with 5% CO2. Doubling time: ca. 4 days.,,,,2016-08-24,2017-05-02
51117,HBL-100,,,"CLO_0003626",,,"CLS Cell Lines Service 300178",Homo sapiens,breast,,epithelial,,female,27,Caucasian,,not diseased,,"From CLS: The epithelial cell line HBL-100 has been derived by E.V. Gaffney and associates from the milk of a nursing mother and obtained 3 days after delivery. Although there was no evidence of a breast lesion in the milk donor, and the patient had no family history of breast cancer, the karyotype of the recovered cells was abnormal as early as passage 7. This line was able to synthesize a small amount of lactose and would respond to prolactin or estrogen by producing increased amounts of casein. Electron micrographs revealed microvilli, tonofibrils and desmosomes.",none,,,"DNA Profile (STR, source: CLS): Amelogenin: X,Y CSF1PO: 10 D13S317: 12 D16S539: 9,12 D5S818: 11,12 D7S820: 8,12 THO1: 6,8 TPOX: 8 vWA: 16 D3S1358: 14,16 D21S11: 28,30 D18S51: 16 Penta E: 7 Penta D: 12 D8S1179: 12,15 FGA: 25","adherent; From CLS: monolayer, adherent","From CLS: Medium: DMEM supplemented with 4.5 g/L glucose, L-glutamine, and 10% fetal bovine serum. Subculture: Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask); the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. Split Ratio: A ratio of 1:2 is recommended. Fluid Renewal: 2 to 3 times weekly.",,,,2016-08-24,2016-08-24
51118,JIMT-1,,,,,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 589",Homo sapiens,breast,,epithelial,,female,62,,,"DOID:3005, invasive ductal carcinoma",breast ductal adenocarcinoma,"From DSMZ: established from the pleural effusion of a 62-year-old woman with ductal breast cancer (grade 3 invasive, T2N1M0) after postoperative radiation in 2003; cell line was described to be insensitive to HER-2-inhibiting drugs, e.g. trastuzumab (Herceptin)",none,HER-2 amplification,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 589",,adherent; From DSMZ: epithelial-like cells growing in monolayers,From DSMZ: Medium: 90% Dulbecco's MEM + 10% h.i. FBS. Subculture: Split confluent culture 1:2 to 1:8 every 2-4 days using trypsin/EDTA. Seed out at ca. 1 x 106 cells/80 cm2. Incubation: at 37°C with 5% CO2. Doubling time: ca. 30-40 hours.,"PMID 15634652",,,2016-08-24,2016-08-24
51119,HMT-3522 S1,,,,,,"European Collection of Authenticated Cell Cultures (ECACC) 98102210",Homo sapiens,breast,,epithelial,,female,48,Caucasian,,"DOID:0060082, breast benign neoplasm",,"From ECACC: HMT-3522 S1, also known as HMT-3522/wt, is a subline that has been derived from HMT-3522. The parent line was established from a benign breast tumour of a 48 year old woman and has undergone spontaneous malignant transformation. It was grown on collagen in serum-free media without antibiotics from explantation onwards. HMT-3522 S1 is a continuation of this line from passage 34 growing without collagen.",none,mutation at codon 179 of P53,"European Collection of Authenticated Cell Cultures (ECACC) 98102210",,adherent,From ECACC: DMEM / Ham's F12 (1:1) + 2 mM Glutamine + 250 ng/ml insulin + 10 µg/ml transferrin +10-8 M sodium selenite + 10-10 M 17 beta-estradiol + 0.5 µg/ml hydrocortisone + 5 µg/ml ovine prolactin + 10 ng/ml EGF,"PMID 3558253",,,2016-08-24,2016-08-24
51120,SUM102PT,SUM-102PT,,,,,"Asterand SUM-102PT",Homo sapiens,breast,,,,,,,,"DOID:3005, invasive ductal carcinoma",breast ductal adenocarcinoma,"From Asterand: developed from a patient with minimally invasive, ER negative and PR negative human breast carcinoma.",none,,,,adherent,"From Asterand: Ham’s F-12 + 1 g/L bovine serum albumin (fatty acid-free), 10 ng/mL EGF, 5 mM ethanolamine, 10 mM HEPES, 1 ug/mL hydrocortisone, 5 ug/mL insulin, 50 nM sodium selenite, 5 ug/mL apo-transferrin, 10 nM triiodo-L-thyronine","PMID 10604729",,,2016-08-24,2016-08-24
51121,SUM190PT,SUM-190PT,,,,,"Asterand SUM-190PT",Homo sapiens,breast,,,,,,,,"DOID:6263, inflammatory breast carcinoma",inflammatory luminal breast carcinoma,From Asterand: derived from a primary human inflammatory luminal breast carcinoma,none,,,,adherent,"From Asterand: Ham’s F-12 + 1 g/L bovine serum albumin, 5 mM ethanolamine, 10 mM HEPES, 1 ug/mL hydrocortisone, 5 ug/mL insulin, 50 nM sodium selenite, 5 ug/mL apo-transferrin, 10 nM triiodo-L-thyronine","PMID 10604729",,,2016-08-24,2016-08-24
51122,SUM44PE,SUM-44PE,,,,,"Asterand SUM-44PE",Homo sapiens,breast,,,,,,,,"DOID:3459, breast carcinoma",luminal breast carcinoma,From Asterand: derived from primary human luminal breast epithelial cells of a pleural effusion.,none,,,,adherent,"From Asterand: Ham’s F-12 + 1 g/L bovine serum albumin, 5 mM ethanolamine, 10 mM HEPES, 1 ug/mL hydrocortisone, 5 ug/mL insulin, 50 nM sodium selenite, 5 ug/mL apo-transferrin, 10 nM triiodo-L-thyronine","PMID 10604729",,,2016-08-24,2016-08-24
51123,HMT-3522 T4-2,,,,,,"European Collection of Authenticated Cell Cultures (ECACC) 98102212",Homo sapiens,breast,,epithelial,,female,48,Caucasian,,"DOID:0060082, breast benign neoplasm",,"From ECACC: HMT-3522 T4-2, also known as HMT-3522/mt-1, is a subline that has been derived from HMT-3522. The parent line was established from a benign breast tumour of a 48 year old woman and has undergone spontaneous malignant transformation. It was grown on collagen in serum-free media without antibiotics from explantation onwards. A mutation at codon 179 of the p53 gene was detected during establishment of the parent line at passage 25-30. The subline S1 is a continuation of this line from passage 34 (ECACC cataogue no. 98102210) growing without collagen. Subline S2 (ECACC catalogue no. 98102211) has been isolated by culturing S1 cells from passage 118 in serum-free media without epidermal growth factor (EGF). The third subline, T4-2 was established from S2 at passage 238 and is the only tumorigenic line in nude mice out of the 3 sublines.",none,mutation at codon 179 of P53; trisomy for the short arm of chromosome 7,"European Collection of Authenticated Cell Cultures (ECACC) 98102212","DNA Profile (STR, source: Asterand): STR-PCR Data: Amelogenin: X CSF1PO: 11,12 D13S317: 11 D16S539: 11,13 D5S818: 9,11 D7S 820: 8,9 THO1: 9,9.3 TPOX: 8,11 vWA: 14,17",adherent,From ECACC: DMEM / Ham's F12 (1:1) + 2 mM Glutamine + 250 ng/ml insulin + 10 µg / ml transferrin +10-8 M Sodium selenite + 10-10 M 17 beta-estradiol + 0.5 µg/ml hydrocortisone + 5 µg / ml ovine prolactin,"PMID 3558253",,,2016-08-24,2016-08-24
51124,MCF 10A-H2B-mCherry,,,"CLO_0007599",MCF 10A,HMSL50583-7,Mario Niepel (Harvard Medical School),Homo sapiens,breast,,epithelial,,female,,,,fibrocystic disease,non-tumorigenic,"Modification type: retroviral transformation with an H2B-mCherry expression cassette that comprised AAVS1 homology arms, the hPGK promotor, and SV40 polyA terminator and integration into the AAVS1 safe harbor genomic locus using CRISPR/Cas9; followed by clonal selection based on transgene expression level. Citations: H2B-mCherry expression cassette: gift of Robert Benezra (Memorial Sloan Kettering Cancer Center), Addgene plasmid #20972; SV40 polyA terminator: gift from Rudolf Jaenisch (Whitehead Institute for Biomedical Research), Addgene plasmid # 22072.",H2B-mCherry integration at AAVS1 safe harbor locus,,,,adherent,,"PMID 27135972",,,2016-08-24,2016-08-24
51125,184A1 F3aN400-Venus NLS-mCherry,,,"CLO_0001137",184A1,HMSL51088-2,Somponnat Sampattavanich (Mahidol University),Homo sapiens,breast,,,,,,,,,,"Modification type: stable retroviral transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated constructs: an NLS-mCherry expression cassette and pMSCV-puro-FoxO3aN400-Venus, a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479) inserted upstream of the Venus sequence in pMSCV-puro.",NLS-mCherry and pMSCV-puro-FoxO3aN400-Venus integration,,,,adherent,,,,,2017-05-02,2017-05-02
51126,MCF 10A F3aN400-Venus-P2A-NLS-mCherry,,,"CLO_0007599",MCF 10A,HMSL50583-11,Somponnat Sampattavanich (Mahidol University),Homo sapiens,breast,,epithelial,,female,,,,fibrocystic disease,non-tumorigenic,"Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing FoxO3aN400-HA-Venus-P2A-NLS-Myc-mCherry, which includes a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479)",pPB-CAG-FoxO3aN400-HA-Venus-P2A-NLS-Myc-mCherry,,,,adherent,,,,,2017-05-02,2017-05-02
51127,MCF 10A F3aN400S294A/S344A-Venus-P2A-NLS-mCherry,,,"CLO_0007599",MCF 10A,HMSL50583-11,Somponnat Sampattavanich (Mahidol University),Homo sapiens,breast,,epithelial,,female,,,,fibrocystic disease,non-tumorigenic,"Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing FoxO3aN400S294A/S344A-HA-Venus-NLS-Myc-mCherry, which includes a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479) and mutated ERK phosphorylation sites S294A/S344A",pPB-CAG-FoxO3aN400S294A/S344A-HA-Venus-P2A-NLS-Myc-mCherry,,,,adherent,,,,,2017-05-02,2017-05-02
51128,MCF 10A F3aN400T32A/S253A/S315A-Venus-P2A-NLS-mCherry,,,"CLO_0007599",MCF 10A,HMSL50583-11,Somponnat Sampattavanich (Mahidol University),Homo sapiens,breast,,epithelial,,female,,,,fibrocystic disease,non-tumorigenic,"Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry, which includes a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479) and mutated AKT phosphorylation sites T32A/S253A/S315A",pPB-CAG-FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry,,,,adherent,,,,,2017-05-02,2017-05-02
51129,184A1 F3aN400-Venus-P2A-NLS-mCherry,,,"CLO_0001137",184A1,HMSL51088-3,Somponnat Sampattavanich (Mahidol University),Homo sapiens,breast,,,,,,,,,,"Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing FoxO3aN400-HA-Venus-P2A-NLS-Myc-mCherry, which includes a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479)",pPB-CAG-FoxO3aN400-HA-Venus-P2A-NLS-Myc-mCherry,,,,adherent,,,,,2017-05-02,2017-05-02
51130,184A1 F3aN400S294A/S344A-Venus-P2A-NLS-mCherry,,,"CLO_0001137",184A1,HMSL51088-3,Somponnat Sampattavanich (Mahidol University),Homo sapiens,breast,,,,,,,,,,"Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing FoxO3aN400S294A/S344A-HA-Venus-P2A-NLS-Myc-mCherry, which includes a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479) and mutated ERK phosphorylation sites S294A/S344A",pPB-CAG-FoxO3aN400S294A/S344A-HA-Venus-P2A-NLS-Myc-mCherry,,,,adherent,,,,,2017-05-02,2017-05-02
51131,184A1 F3aN400T32A/S253A/S315A-Venus-P2A-NLS-mCherry,,,"CLO_0001137",184A1,HMSL51088-3,Somponnat Sampattavanich (Mahidol University),Homo sapiens,breast,,,,,,,,,,"Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry, which includes a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479) and mutated AKT phosphorylation sites T32A/S253A/S315A",pPB-CAG-FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry,,,,adherent,,,,,2017-05-02,2017-05-02
51132,MCF 10A EKAREV-P2A-F3aN400-mCherry,,,"CLO_0007599",MCF 10A,HMSL50583-12,Somponnat Sampattavanich (Mahidol University),Homo sapiens,breast,,epithelial,,female,,,,fibrocystic disease,non-tumorigenic,"Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing EKAREV-P2A-FoxO3aN400-HA-mCherry, which includes the ERK FRET/CFP reporter EKAREV (PMID: 23219535; PMID: 24140422), a P2A site, and a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479)",pPB-CAG-EKAREV-P2A-FoxO3aN400-HA-mCherry,,,,adherent,,,,,2017-05-02,2017-05-02
51133,MCF 10A EKAREV H2B-mCherry,,,"CLO_0007599",MCF 10A,HMSL50583-13,Somponnat Sampattavanich (Mahidol University),Homo sapiens,breast,,epithelial,,female,,,,fibrocystic disease,non-tumorigenic,"Modification type: stable retroviral transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated constructs: an H2B-mCherry expression cassette and pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing the ERK FRET/CFP reporter EKAREV (PMID: 23219535; PMID: 24140422)",pPB-CAG-EKAREV,,,,adherent,,,,,2017-05-02,2017-05-02
51134,PDX1258,,,,,,Dan Stover (Harvard Medical School),Homo sapiens,breast,,epithelial,,female,,,,,metaplastic triple negative breast cancer,,none,,,"DNA Profile (STR): Amelogenin: X CSF1PO: 11 D13S317: 7 D16S539: 13 D5S818: 13 D7S820: 9,10 THO1: 8 TPOX: 8,11 vWA: 16",adherent,"Cells are cultured in F-media: 3:1 mix of complete Dulbecco's MEM (containing 10% fetal bovine serum, 1% penicillin/streptomycin) and Ham's F12 suplemented with 0.125 ng/ml epidermal growth factor, 25 ng/ml hydrocortisone, 5 ug/ml insulin, 8.6 ng/ml cholera toxin, and 5 uM Y-27632 (HMSLID 10176). Protocol: Collect spent culture medium in a falcon tube, wash cells once with PBS and collect wash, trypsinize cells with 0.25% trypsin, collect trypsinized cells. Centrifuge at 300 x g for 5 min. Resuspend the cell pellet in F-media, and transfer to fresh plates or flasks. A cubcultivation ratio of 1:2 to 1:4 recommended.",,,"Established by Dan Stover (Joan Brugge Lab, Harvard Medical School) from a PDX tumor (Jean Zhao Lab, Dana Farber Cancer Institute, established by Elgene Lim)",2018-03-27,2018-03-27
51135,PDX1328,,,,,,Caitlin Mills (Harvard Medical School),Homo sapiens,breast,,epithelial,,female,,,,,breast cancer,,none,,,"DNA Profile (STR): Amelogenin: X CSF1PO: 11,14 D13S317: 8,13 D16S539: 11 D5S818: 11,12 D7S820: 10,11,12 THO1: 6,9 TPOX: 8,9 vWA: 16,19",adherent,"Cells are cultured in F-media: 3:1 mix of complete Dulbecco's MEM (containing 10% fetal bovine serum, 1% penicillin/streptomycin) and Ham's F12 suplemented with 0.125 ng/ml epidermal growth factor, 25 ng/ml hydrocortisone, 5 ug/ml insulin, 8.6 ng/ml cholera toxin, and 5 uM Y-27632 (HMSLID 10176). Protocol: Collect spent culture medium in a falcon tube, wash cells once with PBS and collect wash, trypsinize cells with 0.25% trypsin, collect trypsinized cells. Centrifuge at 300 x g for 5 min. Resuspend the cell pellet in F-media, and transfer to fresh plates or flasks. A cubcultivation ratio of 1:2 to 1:4 recommended.",,,"Established by Caitlin Mills (Harvard Medical School) from a PDX tumor (Jean Zhao Lab, Dana Farber Cancer Institute, established by Elgene Lim)",2018-03-27,2018-03-27
51136,PDXHCI002,,,,,,Dan Stover (Harvard Medical School),Homo sapiens,breast,,epithelial,,female,,,,"DOID: 3008, IDC","poorly differentiated, medullary type, stage 3A",,none,,,"DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 9,12 D16S539: 12 D5S818: 12 D7S820: 8,11 THO1: 6,8 TPOX: 8,11 vWA: 17,19",adherent,"Cells are cultured in F-media: 3:1 mix of complete Dulbecco's MEM (containing 10% fetal bovine serum, 1% penicillin/streptomycin) and Ham's F12 suplemented with 0.125 ng/ml epidermal growth factor, 25 ng/ml hydrocortisone, 5 ug/ml insulin, 8.6 ng/ml cholera toxin, and 5 uM Y-27632 (HMSLID 10176). Protocol: Aspirate spent culture medium, wash cells once with PBS, trypsinize cells with 0.25% trypsin, collect trypsinized cells. Centrifuge at 300 x g for 5 min. Resuspend the cell pellet in F-media, and transfer to fresh plates or flasks. A cubcultivation ratio of 1:2 to 1:6 recommended.","PMID 22019887",,"Established by Dan Stover (Joan Brugge Lab, Harvard Medical School) from a PDX tumor (Alana Welm Lab, University of Utah)",2018-03-27,2018-03-27
51137,PDX1206,,,,,,Caitlin Mills (Harvard Medical School),Homo sapiens,breast,,epithelial,,female,,,,,,,none,,,"DNA Profile (STR): Amelogenin: X CSF1PO: 11 D13S317: 7 D16S539: 13 D5S818: 13 D7S820: 9,10 THO1: 8 TPOX: 8,11 vWA: 16",adherent,"Cells are cultured in F-media: 3:1 mix of complete Dulbecco's MEM (containing 10% fetal bovine serum, 1% penicillin/streptomycin) and Ham's F12 suplemented with 0.125 ng/ml epidermal growth factor, 25 ng/ml hydrocortisone, 5 ug/ml insulin, 8.6 ng/ml cholera toxin, and 5 uM Y-27632 (HMSLID 10176). Protocol: Collect spent culture medium in a falcon tube, wash cells once with PBS and collect wash, trypsinize cells with 0.25% trypsin, collect trypsinized cells. Centrifuge at 300 x g for 5 min. Resuspend the cell pellet in F-media, and transfer to fresh plates or flasks. A cubcultivation ratio of 1:2 to 1:4 recommended.",,,Established by Caitlin Mills (Harvard Medical School),2020-03-02,2020-03-02
51138,EVSA-T,,,,,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 433",Homo sapiens,breast,,epithelial,,female,,,,,,,none,,"906862",,adherent,"From the vendor: Cells are cultured in EMEM + 10% h.i. FBS + 2 mM L-glutamine. Protocol: split semi-confluent culture 1:3 every 3-4 days using trypsin/EDTA, dissociate cells with a pipette (10 ml) during trypsination; seed out at ~ 2 x 10^6 cells/25 cm2 in 10 ml medium; maximal density at ~0.6 x 10^6/cm2; after thawing, viability may be low (about 50%) for the first week. Maintain cultures at 37 °C with 5% CO2.",,,,2020-03-02,2020-03-02
51139,MDAMB330,,,,,,"ATCC HTB-127",Homo sapiens,breast,,epithelial,,female,,,,,,,none,,"1330941",,adherent,"From the vendor: Cells are cultured in Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with: 30 ng/ ml human recombinant EGF, 0.016 mg/ml human recombinant insulin, 2mM glutathione, 20% fetal bovine serum. Protocol: Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 to 2 minutes, remove trypsin and let the culture sit at 37C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. A subcultivation ratio of 1:2 to 1:4 is recommended. Maintain cultures at 37˚C free of CO2.",,,,2020-03-02,2020-03-02
51140,MGH312,,,,,,Massachusetts General Hospital,Homo sapiens,breast,,epithelial,,female,,,,,,Cell line established from liver metastasis,none,,,,adherent,"Cells are cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillin/streptomycin. Protocol: Aspirate spent medium, wash cells once with PBS, trypsinize cells with 0.25% trypsin. Suspend the trypsinized cells in growth media, and transfer to fresh plates or flasks. A cubcultivation ratio of 1:2 to 1:4 recommended.",,,Established at MGH from patient sample,2020-03-02,2020-03-02
51141,MGH358,,,,,,Massachusetts General Hospital,Homo sapiens,breast,,epithelial,,female,,,,,,,none,"BRCA2, PIK3CA",,,adherent,"Cells are cultured in RPMI-1640 supplemented with 10% FBS and 1% penicillin/streptomycin. Protocol: Aspirate spent medium, wash cells once with PBS, trypsinize cells with 0.25% trypsin. Suspend the trypsinized cells in growth media, and transfer to fresh plates or flasks. A cubcultivation ratio of 1:2 to 1:4 recommended.",,,Established at MGH from patient sample,2020-03-02,2020-03-02