MCF 10A EKAREV H2B-mCherry - Cell Line (ID:51133)
| HMS LINCS ID: | 51133 |
| Name: | MCF 10A EKAREV H2B-mCherry |
| Alternative Names: | |
| LINCS ID: | |
| Alternative ID: | CLO_0007599 |
| Parent Cell Line: | HMSL50583-13: MCF 10A |
| Reference Source: | Somponnat Sampattavanich (Mahidol University) |
| Organism: | Homo sapiens |
| Organ: | breast |
| Tissue: | |
| Cell Type: | epithelial |
| Details of Cell Type: | |
| Donor Sex: | female |
| Donor Age: | |
| Donor Ethnicity: | |
| Donor Health Status: | |
| Disease: | fibrocystic disease |
| Details of Disease: | non-tumorigenic |
| Production Details: | Modification type: stable retroviral transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated constructs: an H2B-mCherry expression cassette and pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing the ERK FRET/CFP reporter EKAREV (PMID: 23219535; PMID: 24140422) |
| Genetic Modification(s): | pPB-CAG-EKAREV |
| Known Mutations: | |
| Citation Information for Mutations: | |
| Verification Reference Profile: | |
| Growth Properties: | adherent |
| Recommended Culture Conditions: | |
| Relevant Citations: | |
| Usage Note: | |
| Comments: | |
| Date Publicly Available: | 2017-05-02 |
| Most Recent Update: | 2017-05-02 |
Datasets:
| HMS Dataset ID | Dataset Title | HMS Dataset Type |
|---|---|---|
| 20300 | Growth factor-induced ERK activity pulsing dynamics in a mammary epithelial cell line. Dataset 1 of 3: single-cell reporter measurements. | Microscopy/Imaging |
| 20301 | Growth factor-induced ERK activity pulsing dynamics in a mammary epithelial cell line. Dataset 2 of 3: single-cell reporter pulsing metrics. | Analysis |
| 20302 | Growth factor-induced ERK activity pulsing dynamics in a mammary epithelial cell line. Dataset 3 of 3: mean reporter pulsing metrics. | Analysis |
Batch Information:
| HMS LINCS Batch ID | Provider | Provider Catalog ID | Provider Batch ID |
|---|---|---|---|
| 51133-1 | Somponnat Sampattavanich (Mahidol University) |