184A1 F3aN400T32A/S253A/S315A-Venus-P2A-NLS-mCherry - Cell Line (ID:51131)

HMS LINCS ID: 51131
Name: 184A1 F3aN400T32A/S253A/S315A-Venus-P2A-NLS-mCherry
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LINCS ID:
Alternative ID: CLO_0001137
Parent Cell Line: HMSL51088-3: 184A1
Reference Source: Somponnat Sampattavanich (Mahidol University)
Organism: Homo sapiens
Organ: breast
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Production Details: Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry, which includes a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479) and mutated AKT phosphorylation sites T32A/S253A/S315A
Genetic Modification(s): pPB-CAG-FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry
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Growth Properties: adherent
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Date Publicly Available: 2017-05-02
Most Recent Update: 2017-05-02

Datasets:

HMS Dataset ID Dataset Title HMS Dataset Type
20290
Growth factor-induced FoxO3a nuclear-cytoplasmic pulsing dynamics of non-phosphorylatable reporters in mammary epithelial cell lines. Dataset 1 of 3: single-cell reporter measurements.
Microscopy/Imaging
20291
Growth factor-induced FoxO3a nuclear-cytoplasmic pulsing dynamics of non-phosphorylatable reporters in mammary epithelial cell lines. Dataset 2 of 3: single-cell reporter pulsing metrics.
Analysis
20292
Growth factor-induced FoxO3a nuclear-cytoplasmic pulsing dynamics of non-phosphorylatable reporters in mammary epithelial cell lines. Dataset 3 of 3: mean reporter pulsing metrics.
Analysis

Batch Information:

HMS LINCS Batch ID Provider Provider Catalog ID Provider Batch ID
51131-1 Somponnat Sampattavanich (Mahidol University)