MCF 10A F3aN400T32A/S253A/S315A-Venus-P2A-NLS-mCherry - Cell Line (ID:51128)

HMS LINCS ID: 51128
Name: MCF 10A F3aN400T32A/S253A/S315A-Venus-P2A-NLS-mCherry
Alternative Names:
LINCS ID:
Alternative ID: CLO_0007599
Parent Cell Line: HMSL50583-11: MCF 10A
Reference Source: Somponnat Sampattavanich (Mahidol University)
Organism: Homo sapiens
Organ: breast
Tissue:
Cell Type: epithelial
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: fibrocystic disease
Details of Disease: non-tumorigenic
Production Details: Modification type: stable piggyBac transposon-mediated transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated construct: pPB-CAG.EBNXN (A. Bradley, Sanger Institute) containing FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry, which includes a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479) and mutated AKT phosphorylation sites T32A/S253A/S315A
Genetic Modification(s): pPB-CAG-FoxO3aN400T32A/S253A/S315A-HA-Venus-P2A-NLS-Myc-mCherry
Known Mutations:
Citation Information for Mutations:
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions:
Relevant Citations:
Usage Note:
Comments:
Date Publicly Available: 2017-05-02
Most Recent Update: 2017-05-02

Datasets:

HMS Dataset ID Dataset Title HMS Dataset Type
20290
Growth factor-induced FoxO3a nuclear-cytoplasmic pulsing dynamics of non-phosphorylatable reporters in mammary epithelial cell lines. Dataset 1 of 3: single-cell reporter measurements.
Microscopy/Imaging
20291
Growth factor-induced FoxO3a nuclear-cytoplasmic pulsing dynamics of non-phosphorylatable reporters in mammary epithelial cell lines. Dataset 2 of 3: single-cell reporter pulsing metrics.
Analysis
20292
Growth factor-induced FoxO3a nuclear-cytoplasmic pulsing dynamics of non-phosphorylatable reporters in mammary epithelial cell lines. Dataset 3 of 3: mean reporter pulsing metrics.
Analysis

Batch Information:

HMS LINCS Batch ID Provider Provider Catalog ID Provider Batch ID
51128-1 Somponnat Sampattavanich (Mahidol University)