184A1 F3aN400-Venus NLS-mCherry - Cell Line (ID:51125)
HMS LINCS ID: | 51125 |
Name: | 184A1 F3aN400-Venus NLS-mCherry |
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LINCS ID: | |
Alternative ID: | CLO_0001137 |
Parent Cell Line: | HMSL51088-2: 184A1 |
Reference Source: | Somponnat Sampattavanich (Mahidol University) |
Organism: | Homo sapiens |
Organ: | breast |
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Production Details: | Modification type: stable retroviral transformation followed by sorting for cells with tightly binned intensity cutoffs for both fluorescent proteins; integrated constructs: an NLS-mCherry expression cassette and pMSCV-puro-FoxO3aN400-Venus, a fragment of human FoxO3a spanning residues 1-400 and carrying a loss-of-function H212R mutation in the DNA binding domain (PMID: 11964479) inserted upstream of the Venus sequence in pMSCV-puro. |
Genetic Modification(s): | NLS-mCherry and pMSCV-puro-FoxO3aN400-Venus integration |
Known Mutations: | |
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Growth Properties: | adherent |
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Date Publicly Available: | 2017-05-02 |
Most Recent Update: | 2017-05-02 |
Datasets:
HMS Dataset ID | Dataset Title | HMS Dataset Type |
---|---|---|
20287 | FoxO3a nuclear-cytoplasmic pulsing dynamics in a mammary epithelial cell line following growth-factor treatment with varying ERK/AKT activation loads. Dataset 1 of 3: single-cell reporter measurements. | Microscopy/Imaging |
20288 | FoxO3a nuclear-cytoplasmic pulsing dynamics in a mammary epithelial cell line following growth-factor treatment with varying ERK/AKT activation loads. Dataset 2 of 3: single-cell reporter pulsing metrics. | Analysis |
20289 | FoxO3a nuclear-cytoplasmic pulsing dynamics in a mammary epithelial cell line following growth-factor treatment with varying ERK/AKT activation loads. Dataset 3 of 3: mean reporter pulsing metrics. | Analysis |
Batch Information:
HMS LINCS Batch ID | Provider | Provider Catalog ID | Provider Batch ID |
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51125-1 | Somponnat Sampattavanich (Mahidol University) |