WM-115 - Cell Line (ID:50567)

HMS LINCS ID: 50567
Name: WM-115
Alternative Names:
LINCS ID: LCL-1260
Alternative ID: CLO_0009613
Parent Cell Line:
Reference Source: ATCC CRL-1675
Organism: Homo sapiens
Organ: skin
Tissue:
Cell Type:
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:1909, malignant melanoma
Details of Disease:
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS909784
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.03% EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 34 to 35°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 34 to 35°C. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Relevant Citations:
Usage Note:
Comments:
Date Publicly Available: 2012-07-31
Most Recent Update: 2016-04-04

Datasets:

HMS Dataset ID Dataset Title HMS Dataset Type
20217
Viability and apoptosis in BRAF(V600E/D) melanoma cell lines monitored by imaging
Microscopy/Imaging
20218
Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA)
Reverse Phase Protein Array
20219
Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging
Microscopy/Imaging
20229
PLSR model loadings (pMEK and pERK included) from analysis of the covariation of molecular signals with cell viability and apoptosis fraction in BRAF(V600E/D) melanoma cell lines
Analysis
20230
PLSR model loadings (pMEK and pERK excluded) from analysis of the covariation of molecular signals with cell viability and apoptosis fraction in BRAF(V600E/D) melanoma cell lines
Analysis
20231
Average variable importance in the projection (VIP) scores from the PLSR models analyzing the covariation of molecular signals with cell viability and apoptosis fraction in BRAF(V600E/D) melanoma cell lines
Analysis
20272
Viability and apoptosis measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (viability/apoptosis dataset 1 of 2)
Microscopy/Imaging
20273
Viability and apoptosis measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of three compounds (viability/apoptosis dataset 2 of 2)
Microscopy/Imaging
20274
Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4)
Microscopy/Imaging
20275
Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 2 of 4)
Microscopy/Imaging
20276
Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4)
Microscopy/Imaging
20277
Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4)
Microscopy/Imaging

Batch Information:

HMS LINCS Batch ID Provider Provider Catalog ID Provider Batch ID
50567-1 Nathan Moerke (Harvard Medical School)
50567-2 Nathan Moerke (Harvard Medical School)
50567-3 Nathan Moerke (Harvard Medical School)