BT-474 - Cell Line (ID:50106)

HMS LINCS ID: 50106
Name: BT-474
Alternative Names:
LINCS ID: LCL-1308
Alternative ID: CLO_0002042
Parent Cell Line:
Reference Source: ATCC HTB-20
Organism: Homo sapiens
Organ: breast
Tissue:
Cell Type:
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3008, ductal carcinoma
Details of Disease: breast ductal carcinoma
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS946359
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: Hybri-Care Medium ATCC 46-X, 90%; fetal bovine serum, 10%. Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Relevant Citations:
Usage Note:
Comments:
Date Publicly Available: 2012-07-31
Most Recent Update: 2016-04-04

Batch Information for HMSL50106-2:

HMS LINCS Batch ID: 50106-2
Provider: ATCC
Provider Catalog ID: HTB-20
Provider Batch ID:
Source Information: Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010
Date Received: 2010-07-16
HMS QC Outcome:
Transient Modification(s):
Comments:
Date Publicly Available: 2016-06-06
Most Recent Update: 2016-07-12