T24 - Cell Line (ID:50048)

HMS LINCS ID: 50048
Name: T24
Alternative Names: T-24
LINCS ID: LCL-1709
Alternative ID: CLO_0009245
Parent Cell Line:
Reference Source: ATCC HTB-4
Organism: Homo sapiens
Organ: urinary bladder
Tissue:
Cell Type: epithelial
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:4007, bladder carcinoma; DOID:4006, bladder transitional cell carcinoma
Details of Disease: transitional cell carcinoma; urothelial bladder carcinoma
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS724812
Verification Reference Profile: DNA Profile (STR, source: ATCC): Amelogenin: X CSF1PO: 10,12 D13S317: 12 D16S539: 9 D5S818: 10,12 D7S820: 10,11 THO1: 6 TPOX: 8,11 vWA: 17
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: McCoy's 5a medium (modified) with 1.5 mM L-glutamine adjusted to contain 2.2 g/L sodium bicarbonate, 90%; fetal bovine serum, 10%. Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Relevant Citations:
Usage Note:
Comments:
Date Publicly Available: 2012-04-25
Most Recent Update: 2016-07-12

Datasets:

HMS Dataset ID Dataset Title HMS Dataset Type
20003
Tang Mitosis/Apoptosis ver. II
Microscopy/Imaging

Batch Information:

HMS LINCS Batch ID Provider Provider Catalog ID Provider Batch ID
50048-1 Center for Molecular Therapeutics (CMT; Massachusetts General Hospital) MGH-11