| HMS LINCS ID:
|
50048
|
| Name:
|
T24
|
| Alternative Names:
|
T-24
|
| LINCS ID:
|
LCL-1709
|
| Alternative ID:
|
CLO_0009245
|
| Parent Cell Line:
|
|
| Reference Source:
|
ATCC HTB-4
|
| Organism:
|
Homo sapiens
|
| Organ:
|
urinary bladder
|
| Tissue:
|
|
| Cell Type:
|
epithelial
|
| Details of Cell Type:
|
|
| Donor Sex:
|
female
|
| Donor Age:
|
|
| Donor Ethnicity:
|
|
| Donor Health Status:
|
|
| Disease:
|
DOID:4007, bladder carcinoma; DOID:4006, bladder transitional cell carcinoma
|
| Details of Disease:
|
transitional cell carcinoma; urothelial bladder carcinoma
|
| Production Details:
|
|
| Genetic Modification(s):
|
none
|
| Known Mutations:
|
|
| Citation Information for Mutations:
|
COSS724812
|
| Verification Reference Profile:
|
DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 10,12
D13S317: 12
D16S539: 9
D5S818: 10,12
D7S820: 10,11
THO1: 6
TPOX: 8,11
vWA: 17
|
| Growth Properties:
|
adherent
|
| Recommended Culture Conditions:
|
From MGH/CMT as specified by cell provider: McCoy's 5a medium (modified) with 1.5 mM L-glutamine adjusted to contain 2.2 g/L sodium bicarbonate, 90%; fetal bovine serum, 10%.
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
|
| Relevant Citations:
|
|
| Usage Note:
|
|
| Comments:
|
|
| Date Publicly Available:
|
2012-04-25
|
| Most Recent Update:
|
2016-07-12
|