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Insulin-like Growth Factor 1 (IGF-1)
Responses to ligand

General information

Ligand family Ins/IGF ligand
HMS LINCS ID 200855
UniProt ID P05019
Molecular weight 21 kDa
Receptor affinity
Receptor KD
IGF1R 1 nM
IR 10 nM
Affinity references: Lammers, EMBO 1989, Blakesley VA 1996

Data downloads

Time course response

Fold-change response of pERK and pAKT following 10, 30 or 90 minutes treatment with IGF-1 at 1ng/ml (left) or 100ng/ml (right). Colored trajectories represent the indicated cell lines and all remaining cell lines are gray. Non-significant responses (below ~1.2-fold) are set to 1.

Pathway bias

(Left) Distribution of the pathway bias across cell lines for IGF-1 (in black) and 2 reference ligands (FGF-1, blue; and EGF, red) where the bias is defined as the arctangent of the ratio of the pAKT to pERK fold change responses (maximum across the three time points at 100ng/ml). (Right) Distribution of the pathway bias for all ligands that exhibited a least 50% significant responses across all cell lines when stimulated at 100ng/ml. Ligands are sorted according the median of the pathway bias and colored as in the left panel.

Dose response

Fold change pERK (left) and pAKT (right) responses following treatment with IGF-1 at 1ng/ml and 100ng/ml (maximum response across 10, 30 and 90 minutes). Colored trajectories represent the indicated cell lines and all remaining cell lines are gray. Non-significant responses (below ~1.2-fold) are set to 1.

Sensitivity class

Distribution of sensitivity classes by ligands for pERK (left) and pAKT (right): ‘Equal’ (red) means that the fold change response at low dose is at least 75% of the high dose response; ‘100ng>1ng’ (blue) means that low dose response is statistically significant but less than 75% of the high dose response; ‘only 100ng’ (yellow) means that only the high dose response is significant.

Kinetic cluster

Distribution of the kinetics of the responses at 100mg/ml for IGF-1 (left) and the different ligand families (ErbB ligands, Ins/IGF ligands, FGF-1/2, and all remaining ligands) for pAKT (top) and pERK (bottom). Kinetics classes are determined by an unsupervised k-means clustering of the time-series data that identified trajectories that correspond to sustained, transient, late, or no response.

When using any of the data or analysis presented here, please cite Niepel et al., BMC Biology, 2014. For more information, contact Mario Niepel (mario_niepel@hms.harvard.edu).