HMS LINCS ID,Name,Alternative Names,LINCS ID,Alternative ID,Parent Primary Cell,Parent Primary Cell HMS LINCS ID,Citation Information for Mutations,Molecular Features,Passaging Method,Passage Last Karyotyping,Recommended Culture Conditions,Molecular Features,Related Projects,Cell Markers,Genetic Modification(s),Production Details,Comments,Date Publicly Available,Most Recent Update
70001,Axiogenesis iPSC,"iPSCs derived from human dermal fibroblasts by Ncardia, previously Axiogenesis",,,,,,,,,"Complete culture medium recipe:
450mL IMDM (PAN,P04-20150)
500μL Insulin (Insulin solution human, I9278, Sigma)
50mL Fetal calf serum (heat inactivated)
Thioglycerol (400 μM)BMCC serum free culture medium recipe:
500ml IMDM (PAN,P04-20150)
500μl Insulin (Insulin solution human, I9278, Sigma)
5ml CD-Lipids (CD Lipids concentrate, 11905-031, Gibco)
500μl Trace Elements A ( 25-021-CI, Cellgro)
500μl Trace Elements B ( 99-175-CI, Cellgro)
500μl Trace Elements C (99-176-CI, Cellgro)
250μl Vitamin C ( 100mg/ml Stock)
Thioglycerol (400 μM)",,,,,"generated using the Yamanaka protocol (inducing factors Oct3/4, Sox2, Klf4, c-Myc)",,2017-09-29,2018-03-20