Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 384.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.89 NGFR rabbit IgG(H+L) / donkey NGFR 1015.13
A375-P1
D5
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 308.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.67 NGFR rabbit IgG(H+L) / donkey NGFR 468.47
A375-P1
D6
A-375 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.28 NGFR rabbit IgG(H+L) / donkey NGFR 282.18
A375-P1
D7
A-375 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 422.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.51 NGFR rabbit IgG(H+L) / donkey NGFR 620.99
A375-P1
D8
A-375 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 523.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.91 NGFR rabbit IgG(H+L) / donkey NGFR 465.12
A375-P1
D9
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2728.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 495.24 NGFR rabbit IgG(H+L) / donkey NGFR 132.92
A375-P1
E10
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1734.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 353.69 NGFR rabbit IgG(H+L) / donkey NGFR 119.95
A375-P1
E11
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3098.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 540.77 NGFR rabbit IgG(H+L) / donkey NGFR 567.23
A375-P1
E2
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3238.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 574.69 NGFR rabbit IgG(H+L) / donkey NGFR 547.66
A375-P1
E3
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 539.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.41 NGFR rabbit IgG(H+L) / donkey NGFR 1132.49
A375-P1
E4
A-375 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 445.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.24 NGFR rabbit IgG(H+L) / donkey NGFR 1195.67
A375-P1
E5
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2351.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 431.86 NGFR rabbit IgG(H+L) / donkey NGFR 295.59
A375-P1
E6
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1610.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 333.93 NGFR rabbit IgG(H+L) / donkey NGFR 343.07
A375-P1
E7
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2421.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 433.32 NGFR rabbit IgG(H+L) / donkey NGFR 252.56
A375-P1
E8
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1095.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 214.29 NGFR rabbit IgG(H+L) / donkey NGFR 135.55
A375-P1
E9
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 550.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 170.79 NGFR rabbit IgG(H+L) / donkey NGFR 155.36
A375-P1
F10
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 523.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 171.20 NGFR rabbit IgG(H+L) / donkey NGFR 138.23
A375-P1
F11
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 941.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 206.09 NGFR rabbit IgG(H+L) / donkey NGFR 778.64
A375-P1
F2
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 938.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 214.23 NGFR rabbit IgG(H+L) / donkey NGFR 922.84
A375-P1
F3
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 517.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.94 NGFR rabbit IgG(H+L) / donkey NGFR 1172.91
A375-P1
F4
A-375 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 493.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.91 NGFR rabbit IgG(H+L) / donkey NGFR 1049.55
A375-P1
F5
A-375 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 544.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.98 NGFR rabbit IgG(H+L) / donkey NGFR 308.08
A375-P1
F6
A-375 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 492.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.59 NGFR rabbit IgG(H+L) / donkey NGFR 204.04
A375-P1
F7
A-375 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 497.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.72 NGFR rabbit IgG(H+L) / donkey NGFR 524.06
A375-P1
F8
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 475.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.33 NGFR rabbit IgG(H+L) / donkey NGFR 510.86
A375-P1
F9