Viability and apoptosis measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of three compounds (viability/apoptosis dataset 2 of 2) - Dataset (ID:20273)

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Cell Small Molecule 1 Small Molecule 2 Small Molecule 3 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol 3 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Total Number of Cells (Nuclei) Number of Apoptotic Cells Relative Viability Mean Relative Viability Apoptosis Fraction Mean Apoptosis Fraction
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 0.3160
uM
72
hr
2 755 735 0.0033 0.0020 0.9735 0.9824
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 0.3160
uM
72
hr
1 711 706 0.0008 0.0020 0.9930 0.9824
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 0.3160
uM
72
hr
3 626 614 0.0020 0.0020 0.9808 0.9824
COLO 858 Vemurafenib Trametinib Dasatinib 1.0000 0.1000 0.3160
uM
72
hr
1 618 601 0.0028 0.0029 0.9725 0.9759
COLO 858 Vemurafenib Trametinib Dasatinib 1.0000 0.1000 0.3160
uM
72
hr
2 712 695 0.0028 0.0029 0.9761 0.9759
COLO 858 Vemurafenib Trametinib Dasatinib 1.0000 0.1000 0.3160
uM
72
hr
3 914 895 0.0032 0.0029 0.9792 0.9759
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 3.1600
uM
72
hr
1 707 693 0.0023 0.0030 0.9802 0.9776
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 3.1600
uM
72
hr
3 975 952 0.0038 0.0030 0.9764 0.9776
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 3.1600
uM
72
hr
2 717 700 0.0028 0.0030 0.9763 0.9776
COLO 858 Vemurafenib Trametinib Saracatinib 3.1600 0.3160 3.1600
uM
72
hr
2 839 825 0.0023 0.0038 0.9833 0.9738
COLO 858 Vemurafenib Trametinib Saracatinib 3.1600 0.3160 3.1600
uM
72
hr
1 817 795 0.0037 0.0038 0.9731 0.9738
COLO 858 Vemurafenib Trametinib Saracatinib 3.1600 0.3160 3.1600
uM
72
hr
3 917 885 0.0054 0.0038 0.9651 0.9738
COLO 858 Vemurafenib Trametinib Dasatinib 0.3160 0.0316 0.3160
uM
72
hr
2 788 761 0.0045 0.0042 0.9657 0.9671
COLO 858 Vemurafenib Trametinib Dasatinib 0.3160 0.0316 0.3160
uM
72
hr
1 668 650 0.0030 0.0042 0.9731 0.9671
COLO 858 Vemurafenib Trametinib Dasatinib 0.3160 0.0316 0.3160
uM
72
hr
3 828 797 0.0051 0.0042 0.9626 0.9671
COLO 858 Vemurafenib Trametinib Dasatinib 1.0000 0.1000 1.0000
uM
72
hr
2 1083 1059 0.0040 0.0044 0.9778 0.9751
COLO 858 Vemurafenib Trametinib Dasatinib 1.0000 0.1000 1.0000
uM
72
hr
1 1164 1140 0.0040 0.0044 0.9794 0.9751
COLO 858 Vemurafenib Trametinib Dasatinib 1.0000 0.1000 1.0000
uM
72
hr
3 1006 974 0.0053 0.0044 0.9682 0.9751
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.3160
uM
72
hr
1 849 828 0.0035 0.0045 0.9753 0.9731
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.3160
uM
72
hr
2 975 944 0.0051 0.0045 0.9682 0.9731
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.3160
uM
72
hr
3 1204 1175 0.0048 0.0045 0.9759 0.9731
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 1.0000
uM
72
hr
1 1387 1358 0.0048 0.0049 0.9791 0.9743
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 1.0000
uM
72
hr
2 1237 1209 0.0046 0.0049 0.9774 0.9743
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 1.0000
uM
72
hr
3 923 892 0.0051 0.0049 0.9664 0.9743
COLO 858 Vemurafenib Trametinib Saracatinib 3.1600 0.3160 1.0000
uM
72
hr
3 929 907 0.0037 0.0051 0.9763 0.9739
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