Viability and apoptosis measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of three compounds (viability/apoptosis dataset 2 of 2) - Dataset (ID:20273)

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Cell Small Molecule 1 Small Molecule 2 Small Molecule 3 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol 3 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Total Number of Cells (Nuclei) Number of Apoptotic Cells Relative Viability Mean Relative Viability Apoptosis Fraction Mean Apoptosis Fraction
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 1.0000
uM
 72
hr
 1 9628 474 0.5180 0.5111 0.0492 0.0493
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 3.1600
uM
 72
hr
 2 2274 274 0.1132 0.1203 0.1205 0.1099
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.0000
uM
 72
hr
 2 19509 973 1.0489 1.0000 0.0499 0.0517
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.3160
uM
 72
hr
 3 16548 819 0.8901 0.8521 0.0495 0.0546
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 1.0000
uM
 72
hr
 3 9269 446 0.4993 0.5111 0.0481 0.0493
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 3.1600
uM
 72
hr
 1 2870 305 0.1452 0.1203 0.1063 0.1099
A-375 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.0000
uM
 72
hr
 2 15978 849 0.9722 1.0000 0.0531 0.0508
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.0000
uM
 72
hr
 1 19308 1026 1.0346 1.0000 0.0531 0.0517
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.0000
uM
 72
hr
 3 17086 890 0.9165 1.0000 0.0521 0.0517
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.3160
uM
 72
hr
 2 16146 916 0.8618 0.8521 0.0567 0.0546
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 1.0000
uM
 72
hr
 2 9606 487 0.5160 0.5111 0.0507 0.0493
A-375 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.3160
uM
 72
hr
 3 5232 302 0.3168 0.2809 0.0577 0.0601
A-375 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 1.0000
uM
 72
hr
 1 2100 97 0.1287 0.1268 0.0462 0.0491
A-375 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.0000
uM
 72
hr
 1 15785 755 0.9659 1.0000 0.0478 0.0508
A-375 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 1.0000
uM
 72
hr
 2 2015 111 0.1224 0.1268 0.0551 0.0491
A-375 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.3160
uM
 72
hr
 1 3999 254 0.2407 0.2809 0.0635 0.0601
A-375 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 1.0000
uM
 72
hr
 3 2110 97 0.1294 0.1268 0.0460 0.0491
A-375 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.0000
uM
 72
hr
 3 17422 897 1.0619 1.0000 0.0515 0.0508
A-375 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 3.1600
uM
 72
hr
 1 3039 126 0.1872 0.1595 0.0415 0.0410
A-375 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 0.3160
uM
 72
hr
 2 4717 279 0.2852 0.2809 0.0591 0.0601
A-375 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 3.1600
uM
 72
hr
 2 2717 113 0.1673 0.1595 0.0416 0.0410
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.3160
uM
 72
hr
 1 15081 869 0.8042 0.8521 0.0576 0.0546
A-375 Vemurafenib Trametinib Dasatinib 0.0000 0.0000 3.1600
uM
 72
hr
 3 2009 80 0.1240 0.1595 0.0398 0.0410
A-375 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 0.0000
uM
 72
hr
 1 17534 924 0.9793 1.0000 0.0527 0.0484
A-375 Vemurafenib Trametinib Defactinib 0.0000 0.0000 3.1600
uM
 72
hr
 3 2021 208 0.1026 0.1203 0.1029 0.1099
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