Viability and apoptosis measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of three compounds (viability/apoptosis dataset 2 of 2) - Dataset (ID:20273)

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Cell Small Molecule 1 Small Molecule 2 Small Molecule 3 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol 3 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Total Number of Cells (Nuclei) Number of Apoptotic Cells Relative Viability Mean Relative Viability Apoptosis Fraction Mean Apoptosis Fraction
A-375 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 1.0000
uM
 72
hr
 3 11320 265 0.6518 0.6666 0.0234 0.0291
MMAC-SF Vemurafenib Trametinib Dasatinib 0.0000 0.0000 3.1600
uM
 72
hr
 3 506 12 0.4674 0.7329 0.0237 0.0348
A-375 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 1.0000
uM
 72
hr
 2 11776 362 0.6729 0.6666 0.0307 0.0291
MMAC-SF Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 0.3160
uM
 72
hr
 3 1446 46 1.3861 1.3106 0.0318 0.0383
MMAC-SF Vemurafenib Trametinib Dasatinib 0.0000 0.0000 1.0000
uM
 72
hr
 3 683 22 0.6254 0.7364 0.0322 0.0453
MMAC-SF Vemurafenib Trametinib PF562271 0.0000 0.0000 0.3160
uM
 72
hr
 1 1192 39 0.9968 1.2637 0.0327 0.0426
A-375 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 1.0000
uM
 72
hr
 1 11842 391 0.6751 0.6666 0.0330 0.0291
A-375 Vemurafenib Trametinib I-BET 0.0000 0.0000 1.0000
uM
 72
hr
 1 10027 340 0.6952 0.5962 0.0339 0.0732
MZ7-mel Vemurafenib Trametinib Dasatinib 0.0000 0.0000 1.0000
uM
 72
hr
 2 649 22 0.1881 0.2356 0.0339 0.0441
MMAC-SF Vemurafenib Trametinib Dasatinib 0.0000 0.0000 3.1600
uM
 72
hr
 1 1031 35 0.9423 0.7329 0.0339 0.0348
MMAC-SF Vemurafenib Trametinib PF562271 0.0000 0.0000 1.0000
uM
 72
hr
 1 794 27 0.6631 0.6202 0.0340 0.0473
MMAC-SF Vemurafenib Trametinib Saracatinib 0.0000 0.0000 0.0000
uM
 72
hr
 3 1203 41 1.1505 1.0000 0.0341 0.0425
MMAC-SF Vemurafenib Trametinib PF562271 0.0000 0.0000 3.1600
uM
 72
hr
 1 751 26 0.6268 0.4801 0.0346 0.0405
A-375 Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 0.3160
uM
 72
hr
 1 15733 557 0.8947 1.0240 0.0354 0.0404
MZ7-mel Vemurafenib Trametinib Dasatinib 0.0000 0.0000 1.0000
uM
 72
hr
 3 759 27 0.2196 0.2356 0.0356 0.0441
MMAC-SF Vemurafenib Trametinib Defactinib 0.0000 0.0000 0.0000
uM
 72
hr
 1 1060 39 0.9858 1.0000 0.0368 0.0416
MMAC-SF Vemurafenib Trametinib PF562271 0.0000 0.0000 3.1600
uM
 72
hr
 3 408 15 0.3398 0.4801 0.0368 0.0405
A-375 Vemurafenib Trametinib Saracatinib 0.0000 0.0000 1.0000
uM
 72
hr
 1 9338 344 0.5342 0.4663 0.0368 0.0378
MMAC-SF Vemurafenib Trametinib Defactinib 0.0000 0.0000 3.1600
uM
 72
hr
 2 671 25 0.6238 0.6505 0.0373 0.0426
MZ7-mel Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 3.1600
uM
 72
hr
 3 2471 93 0.6267 0.9655 0.0376 0.0440
A-375 Vemurafenib Trametinib Saracatinib 0.0000 0.0000 1.0000
uM
 72
hr
 2 7768 292 0.4441 0.4663 0.0376 0.0378
A-375 Vemurafenib Trametinib Saracatinib 0.0000 0.0000 3.1600
uM
 72
hr
 1 8961 339 0.5121 0.3895 0.0378 0.0403
A-375 Vemurafenib Trametinib Saracatinib 0.0000 0.0000 3.1600
uM
 72
hr
 3 4796 182 0.2741 0.3895 0.0379 0.0403
MMAC-SF Vemurafenib Trametinib JNK-IN-8 0.0000 0.0000 0.3160
uM
 72
hr
 1 1293 49 1.2317 1.3106 0.0379 0.0383
MMAC-SF Vemurafenib Trametinib Defactinib 0.0000 0.0000 3.1600
uM
 72
hr
 1 954 37 0.8854 0.6505 0.0388 0.0426
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