HMS LINCS Batch ID,HMS LINCS ID,Name,Alternative Names,LINCS ID,Alternative ID,Reference Source,Organism,Organ,Tissue,Cell Type,Details of Cell Type,Donor Sex,Donor Age,Donor Ethnicity,Donor Health Status,Disease,Details of Disease,Production Details,Genetic Modification(s),Known Mutations,Citation Information for Mutations,Verification Reference Profile,Growth Properties,Recommended Culture Conditions,Relevant Citations,Usage Note,Comments,Provider,Provider Catalog ID,Provider Batch ID,Source Information,Date Received,HMS QC Outcome,Transient Modification(s),Date Publicly Available,Most Recent Update
50029-1,50029,MCF7,,LCL-1460,"CLO_0007606","ATCC HTB-22",Homo sapiens,breast,,epithelial,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS905946","DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15",adherent,"From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1 mM sodium pyruvate and supplemented with 0.01 mg/ml bovine insulin, 90%; fetal bovine serum, 10%.
Protocol: Remove culture medium to a centrifuge tube.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Transfer the cell suspension to the centrifuge tube with the medium and cells from step 1, and centrifuge at approximately 125 x g for 5 to 10 minutes. Discard the supernatant.
Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended",,,,ATCC,HTB-22,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC.,,,,2012-04-25,2016-04-04
50057-1,50057,SK-BR-3,SKBr3,LCL-1475,"CLO_0009034","ATCC HTB-30",Homo sapiens,breast,,epithelial,,female,,,,"DOID:3459, breast carcinoma",,,none,,,"DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 12
D13S317: 11,12
D16S539: 9
D5S818: 9,12
D7S820: 9,12
THO1: 8,9
TPOX: 8,11
vWA: 17",adherent,"ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0 deg C
Subculturing protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin, 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37 deg C.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week",,,,ATCC,HTB-30,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-05-01,2016-07-12
50058-1,50058,MDA-MB-231,MDAMB231; MDA-MB231,LCL-1461,"CLO_0007634","ATCC HTB-26",Homo sapiens,breast,,epithelial,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS905960","DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 12,13
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 8,9
THO1: 7,9.3
TPOX: 8,9
vWA: 15,18",adherent,"ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 37.0 deg C
Subculturing protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37 deg C without CO2.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week",,,,ATCC,HTB-26,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-05-01,2016-07-12
50091-1,50091,AU565,AU-565,LCL-1462,"CLO_0001769","ATCC CRL-2351",Homo sapiens,breast,,,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS910704",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Subculturing: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended",,,,ATCC,CRL-2351,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50105-1,50105,BT-20,,LCL-1476,"CLO_0002041","ATCC HTB-19",Homo sapiens,breast,,,,female,,,,"DOID:3459, breast carcinoma",breast ductal carcinoma,,none,,"COSS906801",,adherent,"From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended",,,,ATCC,HTB-19,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50106-1,50106,BT-474,,LCL-1308,"CLO_0002042","ATCC HTB-20",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS946359",,adherent,"From MGH/CMT as specified by cell provider: Hybri-Care Medium ATCC 46-X, 90%; fetal bovine serum, 10%.
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended",,,,ATCC,HTB-20,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50107-1,50107,BT-483,,LCL-1309,"CLO_0002043","ATCC HTB-121",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS949093",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.01 mg/ml bovine insulin, 80%; fetal bovine serum, 20%.
Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 3 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 is recommended",,,,ATCC,HTB-121,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50108-1,50108,BT-549,,LCL-1310,"CLO_0002044","ATCC HTB-122",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS905951",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 0.023 IU/ml insulin and 10% fetal bovine serum.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended",,,,ATCC,HTB-122,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50131-1,50131,CAMA-1,,LCL-1466,"CLO_0002194","ATCC HTB-21",Homo sapiens,breast,,,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS946382",,adherent,"From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:4 is recommended",,,,ATCC,HTB-21,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50205-1,50205,HCC1143,,LCL-1329,"CLO_0003630","ATCC CRL-2321",Homo sapiens,breast,,,,female,,,,"DOID:3008, primary ductal carcinoma",breast ductal carcinoma,,none,,"COSS749710",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol: Remove medium, rinse with 0.25% trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended",,,,ATCC,CRL-2321,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50206-1,50206,HCC1395,HCC-1395,LCL-1330,"CLO_0003634","ATCC CRL-2324",Homo sapiens,breast,,,,female,,,,"DOID:3008, primary ductal carcinoma",breast ductal carcinoma,,none,,"COSS749712",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 is recommended",,,,ATCC,CRL-2324,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50207-1,50207,HCC1419,HCC-1419,LCL-1314,"CLO_0003636","ATCC CRL-2326",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS907045",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Growth Conditions: The line grows as large epithelial attached cells in island-like formation.
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended",,,,ATCC,CRL-2326,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50208-1,50208,HCC1428,HCC-1428,LCL-1467,"CLO_0003637","ATCC CRL-2327",Homo sapiens,breast,,,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS1290905",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:8 is recommended",,,,ATCC,CRL-2327,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50210-1,50210,HCC1569,HCC-1569,LCL-1480,"CLO_0003640","ATCC CRL-2330",Homo sapiens,breast,,,,female,,,,"DOID:3459, breast carcinoma",breast carcinoma; metastatic carcinoma,,none,,"COSS907046",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Subculturing: Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended",,,,ATCC,CRL-2330,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50211-1,50211,HCC1806,HCC-1806,LCL-1960,"CLO_0003644","ATCC CRL-2335",Homo sapiens,breast,,,,female,,,,"DOID:7459, primary acantholytic squamous cell carcino",breast ductal carcinoma,,none,,"COSS907047",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Subculturing: Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended",,,,ATCC,CRL-2335,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50212-1,50212,HCC1937,HCC-1937,LCL-1331,"CLO_0003645","ATCC CRL-2336",Homo sapiens,breast,,,,female,,,,"DOID:3008, primary ductal carcinoma",breast ductal carcinoma,,none,,"COSS749714",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol: Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended",,,,ATCC,CRL-2336,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50213-1,50213,HCC1954,HCC-1954,LCL-1332,"CLO_0003647","ATCC CRL-2338",Homo sapiens,breast,,,,female,,,,"DOID:3008, primary ductal carcinoma",breast ductal carcinoma,,none,,"COSS749709",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:8 is recommended",,,,ATCC,CRL-2338,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50214-1,50214,HCC202,HCC-202,LCL-1333,"CLO_0003649","ATCC CRL-2316",Homo sapiens,breast,,,,female,,,,"DOID:3008, primary ductal carcinoma",breast ductal carcinoma,,none,,"COSS1290906",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Subculturing: Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended",,,,ATCC,CRL-2316,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50216-1,50216,HCC38,HCC-38,LCL-1334,"CLO_0003655","ATCC CRL-2314",Homo sapiens,breast,,,,female,,,,"DOID:3008, primary ductal carcinoma",breast ductal carcinoma,,none,,"COSS749717",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended",,,,ATCC,CRL-2314,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50219-1,50219,HCC70,HCC-70,LCL-1335,"CLO_0003658","ATCC CRL-2315",Homo sapiens,breast,,,,female,,,,"DOID:3008, primary ductal carcinoma",breast ductal carcinoma,,none,,"COSS907048",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended",,,,ATCC,CRL-2315,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50238-1,50238,Hs 578T,Hs-578-T,LCL-1315,"CLO_0004009","ATCC HTB-126",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS905957",,adherent,"From MGH/CMT as specified by cell provider: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.01 mg/ml bovine insulin and 10% fetal bovine serum.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended",,,,ATCC,HTB-126,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-07-12
50324-1,50324,MB 157,,LCL-1484,"CLO_0007544","ATCC CRL-7721",Homo sapiens,breast,,,,female,,,,"DOID:3459, breast carcinoma",,,none,,,,adherent,"From MGH/CMT as specified by cell provider: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 90%; fetal bovine serum, 10%.
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: 1:2 to 1:3",,,,ATCC,CRL-7721,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-07-12
50327-1,50327,MDA-MB-134-VI,,LCL-1316,"CLO_0007631","ATCC HTB-23",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,,,lightly adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 80%; fetal bovine serum, 20%.
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended",,,,ATCC,HTB-23,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-07-12
50328-1,50328,MDA-MB-157,,LCL-1916,"CLO_0007632","ATCC HTB-24",Homo sapiens,breast,,,,female,,,,"DOID:5605, breast medullary carcinoma",,,none,,"COSS925338",,adherent,"From MGH/CMT as specified by cell provider: LLeibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C without CO2.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended",,,,ATCC,HTB-24,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50329-1,50329,MDA-MB-175-VII,,LCL-1317,"CLO_0007633","ATCC HTB-25",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS908120",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%.
Protocol:
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended",,,,ATCC,HTB-25,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50331-1,50331,MDA-MB-361,,LCL-1468,"CLO_0007636","ATCC HTB-27",Homo sapiens,breast,,,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS908121",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 80%; fetal bovine serum, 20%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended",,,,ATCC,HTB-27,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50332-2,50332,MDA-MB-415,,LCL-1469,"CLO_0007637","ATCC HTB-128",Homo sapiens,breast,,,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS924240",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2mM L-glutamine supplemented with 10 mcg/ml insulin and 10 mcg/ml glutathione, 85%; fetal bovine serum, 15%.
Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation ratio: A subcultivation ratio of 1:2 is recommended",,,,ATCC,HTB-128,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50333-1,50333,MDA-MB-436,,LCL-1470,"CLO_0007639","ATCC HTB-130",Homo sapiens,breast,,,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS1240172",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 10 mcg/ml insulin, 16 mcg/ml glutathione, 90%; fetal bovine serum, 10%.
Subculturing: Subcultures are prepared by scraping. Remove spent medium, add 3 to 5 ml of fresh medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation ratio: A subcultivation ratio of 1:2 is recommended",,,,ATCC,HTB-130,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50334-1,50334,MDA-MB-453,,LCL-1485,"CLO_0007640","ATCC HTB-131",Homo sapiens,breast,,,,female,,,,"DOID:3459, breast carcinoma",breast carcinoma; metastatic carcinoma,,none,,"COSS908122",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C without CO2.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended",,,,ATCC,HTB-131,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50335-1,50335,MDA-MB-468,,LCL-1471,"CLO_0007641","ATCC HTB-132",Homo sapiens,breast,,,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS908123",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended",,,,ATCC,HTB-132,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50541-1,50541,T47D,T-47D,LCL-1486,"CLO_0009251","ATCC HTB-133",Homo sapiens,breast,,,,female,,,,"DOID:3459, breast carcinoma",,,none,,"COSS905945",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 or DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 37C.",,,,European Collection of Cell Cultures (ECACC),85102201,,Obtained by Joe Gray's group (Oregon Health & Science University) from ECACC,,,,2012-07-31,2016-07-12
50556-1,50556,UACC-812,,LCL-1319,"CLO_0009467","ATCC CRL-1897",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS910910",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with 20 ng/ml human EGF and 20% fetal bovine serum.
Growth Conditions: The cells grow very slowly, and growth is enhanced by using 20% fetal bovine serum and adding epidermal growth factor (20 ng/ml) to the medium.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended",,,,ATCC,CRL-1897,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50557-1,50557,UACC-893,,LCL-1320,"CLO_0009468","ATCC CRL-1902",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS909778",,adherent,"From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended",,,,ATCC,CRL-1902,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-04-04
50574-1,50574,ZR-75-1,,LCL-1321,"CLO_0009727","ATCC CRL-1500",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,,,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended",,,,ATCC,CRL-1500,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-07-31,2016-07-12
50576-1,50576,184B5,,LCL-2081,"CLO_0001138","ATCC CRL-8799",Homo sapiens,breast,,epithelial,,female,,,,not diseased,,"Cells derived from the tissue were exposed to benzo(a)pyrene, and a transformed line was established. The 184B5 cell line was established from normal mammary tissue obtained from a normal reduction mammoplasty.",none,,,,adherent,,,,,ATCC,CRL-8799,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-11-02,2016-04-04
50578-1,50578,HCC1187,,LCL-1324,"CLO_0003632","ATCC CRL-2322",Homo sapiens,breast,duct,epithelial,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS749711",,"mixed, adherent and suspension",,,,,ATCC,CRL-2322,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-11-02,2016-07-12
50583-1,50583,MCF 10A,,LCL-2085,"CLO_0007599","ATCC CRL-10317",Homo sapiens,breast,,epithelial,,female,,,,fibrocystic disease,non-tumorigenic,,none,,,,adherent,,,,,ATCC,CRL-10317,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-11-02,2016-04-04
50584-1,50584,MCF 10F,,LCL-2102,"CLO_0007600","ATCC CRL-10318",Homo sapiens,breast,,epithelial,,female,,,,fibrocystic disease,non-tumorigenic,,none,,,,adherent,,,,,ATCC,CRL-10318,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2012-11-02,2016-04-04
51081-1,51081,SUM1315MO2,SUM-1315MO2,LCL-2066,"CLO_0009916",University of Michigan (http://www.cancer.med.umich.edu/breast_cell/Production/index.html),Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,"PMID 10604729",,,Stephen Ethier (Wayne State University and University of Michigan Medical School),,,Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier,,,,2013-07-30,2016-07-12
51082-1,51082,SUM149PT,SUM-149PT,LCL-2067,"CLO_0009917","Asterand SUM-149PT",Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,"PMID 10604729",,,Stephen Ethier (Wayne State University and University of Michigan Medical School),,,Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier,,,,2013-07-30,2016-07-12
51083-1,51083,SUM159PT,SUM-159PT,LCL-2068,"CLO_0009918","Asterand SUM-159PT",Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,"PMID 10604729",,,Stephen Ethier (Wayne State University and University of Michigan Medical School),,,Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier,,,,2013-07-30,2016-07-12
51084-1,51084,SUM185PE,SUM-185PE,LCL-2069,"CLO_0009919","Asterand SUM-185PE",Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,"PMID 10604729",,,Stephen Ethier (Wayne State University and University of Michigan Medical School),,,Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier,,,,2013-07-30,2016-07-12
51085-1,51085,SUM225CWN,SUM-225CWN,LCL-2070,"CLO_0009920","Asterand SUM-225CWN",Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,"PMID 10604729",,,Stephen Ethier (Wayne State University and University of Michigan Medical School),,,Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier,,,,2013-07-30,2016-07-12
51086-1,51086,SUM229PE,SUM-229PE,LCL-2065,"CLO_0009921","Asterand SUM-229PE",Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,"PMID 10604729",,,Stephen Ethier (Wayne State University and University of Michigan Medical School),,,Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier,,,,2013-07-30,2016-07-12
51087-1,51087,SUM52PE,SUM-52E,LCL-2071,"CLO_0037189","Asterand SUM-52PE",Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,"PMID 10604729",,,Stephen Ethier (Wayne State University and University of Michigan Medical School),,,Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier,,,,2013-07-30,2016-07-12
51088-1,51088,184A1,,LCL-2080,"CLO_0001137","ATCC CRL-8798",Homo sapiens,breast,,,,,,,,,,,none,,,,adherent,,,,,ATCC,CRL-8798,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2013-07-30,2016-07-12
51089-1,51089,MCF12A,MCF-12A,LCL-2082,"CLO_0007603","ATCC CRL-10782",Homo sapiens,breast,,,,,,,,,,,none,,,,adherent,,,,,ATCC,CRL-10782,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2013-07-30,2016-07-12
51090-1,51090,MX1,MX-1,LCL-2072,"CLO_0009915","DTP, DCTD Tumor Repository, National Cancer Institute",Homo sapiens,breast,,,,,,,,,,,none,,,,not specified,,,,,"DTP, DCTD Tumor Repository (National Cancer Institute)",,,"Obtained by Joe Gray's group (Oregon Health & Science University) from the DTP, DCTD Tumor Repository",,,,2013-07-30,2016-07-12
51091-1,51091,T47DKBLUC,T47D-KBluc,LCL-2064,"CLO_0037191","ATCC CRL-2865",Homo sapiens,breast,,,,,,,,,,The parent cell line was transfected with pGL2.TATA.Inr.luc.neo which contains three estrogen responsive elements upstream of a luc reporter gene. The cells were selected for responsiveness to 17-beta-estradiol.,pGL2.TATA.Inr.luc.neo integration,,,,adherent,,,,,ATCC,CRL-2865,,Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC,,,,2013-07-30,2016-07-12
51092-1,51092,600MPE,,LCL-2073,"CLO_0009908",H.S. Smith (California Pacific Medical Center),Homo sapiens,breast,,,,,,,,,,,,,,,,,"PMID 3470538",,,H.S. Smith (California Pacific Medical Center),,,Obtained by Joe Gray's group (Oregon Health & Science University) from H.S. Smith,,,,2013-07-30,2016-07-12
51093-1,51093,HCC2185,,LCL-2074,"CLO_0009909",Adi Gazdar (University of Texas-Southwestern Medical Center),Homo sapiens,breast,,,,female,38,white,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,,,non-adherent,,"PMID 9833771",,,Adi Gazdar (University of Texas-Southwestern Medical Center),,,Obtained by Joe Gray's group (Oregon Health & Science University) from Adi Gazdar,,,,2013-07-30,2016-07-12
51094-1,51094,HCC3153,,LCL-2075,"CLO_0009910",Adi Gazdar (University of Texas-Southwestern Medical Center),Homo sapiens,breast,,,,,,,,,,,,,,,,,,,,Adi Gazdar (University of Texas-Southwestern Medical Center),,,Obtained by Joe Gray's group (Oregon Health & Science University) from Adi Gazdar,,,,2013-07-30,2016-07-12
51095-1,51095,LY2,LY 2,LCL-2076,"CLO_0009913",Mark Lippman (National Cancer Institute),Homo sapiens,breast,,,,,,,,,,"The cell line was selected by increasing the concentration of LY 117018, a potent antiestrogen that inhibits cell growth at concentrations as low as 10(-10) M, in the growth medium in a stepwise manner from 10(-8) to 10(-6) M as the cells become resistant.",none,,,,,,"PMID 4029083",,,Georgetown University,,,Obtained by Joe Gray's group (Oregon Health & Science University) from an unknown researcher at Georgetown University,,,,2013-07-30,2016-07-12
51096-1,51096,ZR75B,,LCL-2077,"CLO_0009924",Mark Lippman (National Cancer Institute),Homo sapiens,breast,,,,,,,,,,,,,,,,,"PMID 7439217",,,Georgetown University,,,Obtained by Joe Gray's group (Oregon Health & Science University) from an unknown researcher at Georgetown University,,,,2013-07-30,2016-07-12
51107-1,51107,21MT-1,,,,Ruth Sager (Dana-Farber Cancer Institute),Homo sapiens,breast,,epithelial,,female,36,,,"DOID:3005, invasive ductal carcinoma",breast ductal adenocarcinoma,established from the pleural effusion of lung metastases of a primary breast tumor,none,,,,adherent,,"PMID 2487147; PMID 1977518",,,"Kornelia Polyak (Dana-Farber Cancer Institute), Ruth Sager (Dana-Farber Cancer Institute)",,,Obtained by Joe Gray's group (Oregon Health & Science University) from Kornelia Polyak and Ruth Sager,,,,2016-08-24,2016-08-24
51108-1,51108,21NT,,,,Ruth Sager (Dana-Farber Cancer Institute),Homo sapiens,breast,,epithelial,,female,36,,,"DOID:3005, invasive ductal carcinoma",breast ductal adenocarcinoma,established from primary breast tumor,none,,,,adherent,,"PMID 1977518",,,"Kornelia Polyak (Dana-Farber Cancer Institute), Ruth Sager (Dana-Farber Cancer Institute)",,,Obtained by Joe Gray's group (Oregon Health & Science University) from Kornelia Polyak and Ruth Sager,,,,2016-08-24,2016-08-24
51109-1,51109,21PT,,,,Ruth Sager (Dana-Farber Cancer Institute),Homo sapiens,breast,,epithelial,,female,36,,,"DOID:3005, invasive ductal carcinoma",breast ductal adenocarcinoma,established from primary breast tumor,none,,,,adherent,,"PMID 1977518",,,"Kornelia Polyak (Dana-Farber Cancer Institute), Ruth Sager (Dana-Farber Cancer Institute)",,,Obtained by Joe Gray's group (Oregon Health & Science University) from Kornelia Polyak and Ruth Sager,,,,2016-08-24,2016-08-24
51110-1,51110,CAL-120,,,"CLO_0002179","Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 459",Homo sapiens,breast,,,,female,43,,,"DOID:3458, breast adenocarcinoma",breast adenocarcinoma,From DSMZ: established from the pleural effusion of a metastatic breast adenocarcinoma in 1991,none,,,,adherent; From DSMZ: heterogenous population with epithelial-like and fibroblast-like adherent cells growing in monolayers,From DSMZ: Medium: 90% Dulbecco's MEM + 10% h.i. FBS. Subculture: split confluent culture 1:2 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/175 cm2. Incubation: at 37°C with 10% CO2. Doubling time: ca. 50-60 hours.,,,,Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures,ACC 459,,Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ,,,,2016-08-24,2016-08-24
51111-1,51111,CAL-148,,,"CLO_0002181","Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 460",Homo sapiens,breast,,epithelioid,,female,58,,,"DOID:3458, breast adenocarcinoma",breast adenocarcinoma,From DSMZ: established from the pleural effusion from a 58-year-old French woman with terminally invasive breast cancer (moderately differentiated ductal adenocarcinoma) after repeated cycles of chemotherapy (including adriamycin and taxol),none,,,,"mixed; From DSMZ: epitheloid cells growing as single cells or as clumps either in suspension or, if supplemented with EGF, in monolayers",From DSMZ: Medium: 80% Dulbecco's MEM + 20% h.i. FBS + 2 mM L-glutamine + EGF (1 µg/100 ml). Subculture: Split confluent culture 1:4 to 1:10 every 3-5 days by agitation. Seed out at ca. 0.2-0.3 x 106 cells/ml (suspension culture) or 0.4-0.8 x 105 cells/cm2 (adherent culture). Maintain at about 0.2-0.7 x 106 cells/ml or equivalent. Cell numbers may be difficult to ascertain due to growth in clumps. Cells grow faster in suspension without EGF. Incubation: at 37°C with 10% CO2. Doubling time: ca. 30-40 hours.,"PMID 7531416",,,Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures,ACC 460,,Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ,,,,2016-08-24,2017-05-02
51112-1,51112,CAL-85-1,,,"CLO_0002190","Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 440",Homo sapiens,breast,,epithelial,,female,35,,,"DOID:3458, breast adenocarcinoma",breast adenocarcinoma,From DSMZ: established from the relapsing invasive galactophoric breast adenocarcinoma resected from a 35-year-old woman during radiotherapy in 1990; cells were described as expressing the multidrug resistance (MDR) gene,none,,,,adherent; From DSMZ: adherent epithelial-like cells growing in monolayers,From DSMZ: Medium: 90% Dulbecco's MEM + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate. Subculture: Split confluent culture 1:2 to 1:3 every 3-4 days using trypsin/EDTA (incubation with trypsin/EDTA for about 10 min). Seed out at ca. 0.03-0.04 x 106 cells/cm2. Maintain at 0.04 x 106 cells/cm2. A significant amount of cell debris is always visible in the culture background which might be due to granula or other intracellular material. Incubation: at 37°C with 5% CO2. Doubling time: ca. 70 hours.,"Fischel, J. L., Formento, P., Lucas, C., Berlion, M., Bizzari, J. P., Milano, G. (1993) Le S9788 dans la réversion de la résistance MDR: Activité comparée à des composés de référence. Bull Cancer 80 : 409.",,,Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures,ACC 440,,Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ,,,,2016-08-24,2016-08-24
51113-1,51113,EFM-19,,,"CLO_0002888","Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 231",Homo sapiens,breast,,epithelioid,,female,50,Caucasian,,"DOID:3007, breast ductal carcinoma",breast ductal adenocarcinoma,"From DSMZ: established from the pleural effusion of a 50-year-old Caucasian woman with breast carcinoma (ductal type, histological grading II) in 1979",none,,,,"mixed; From DSMZ: epitheloid, growing in clusters at first, then as monolayer",From DSMZ: Medium: 90% RPMI 1640 + 10% h.i. FBS. Subculture: Split confluent culture 1:2 to 1:3 twice a week using trypsin/EDTA. Seed out at ca. 2 x 106 cells/25 cm2. Incubation: at 37°C with 5% CO2. Doubling time: about 64 hours.,"PMID 6325325",,,Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures,ACC 231,,Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ,,,,2016-08-24,2017-05-02
51114-1,51114,EFM-192A,,,"CLO_0002889","Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 258",Homo sapiens,breast,,epithelioid,,female,46,Caucasian,,"DOID:3458, breast adenocarcinoma",breast adenocarcinoma,From DSMZ: established from the pleural effusion of the left side from a 46-year-old Caucasian woman with metastasizing breast adenocarcinoma in 1985,none,ERBB2 amplification,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 258",,"adherent; From DSMZ: epitheloid, adherent growing as monolayer",From DSMZ: Medium: 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine. Subculture: Split confluent culture 1:2 to 1:3 once a week using trypsin/EDTA. Seed out at ca. 2 x 106 cells/25 cm2. Incubation: at 37°C with 5% CO2. Doubling time: about 9 days.,,,,Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures,ACC 258,,Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ,,,,2016-08-24,2017-05-02
51115-1,51115,EFM-192B,,,"CLO_0002890","Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 308",Homo sapiens,breast,,epithelioid,,female,46,Caucasian,,"DOID:3458, breast adenocarcinoma",breast adenocarcinoma,From DSMZ: established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma,none,,,,adherent; From DSMZ: epitheloid adherent cells growing as monolayers,From DSMZ: Medium: 80% RPMI 1640 + 20% h.i. FBS. Subculture: Subcultivation recommended before reaching confluence. Split 1:2 to 1:3 once a week using trypsin/EDTA. Seed out at ca. 0.5-1.0 x 105 cells/cm2. Incubation: at 37°C with 5% CO2. Doubling time: ca. 4-5 days.,,,,Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures,ACC 308,,Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ,,,,2016-08-24,2017-05-02
51116-1,51116,EFM-192C,,,"CLO_0002891","Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 311",Homo sapiens,breast,,epithelioid,,female,46,Caucasian,,"DOID:3458, breast adenocarcinoma",breast adenocarcinoma,From DSMZ: established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma,none,ERBB2 amplification,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 311",,adherent; From DSMZ: epitheloid adherent cells growing as monolayers,From DSMZ: Medium: 80% RPMI 1640 + 20% h.i. FBS. Subculture: Optimal split ratio is 1:2 to 1:3 once a week using trypsin/EDTA. Seed out at ca. 5-8 x 104 cells/cm2 or 2 x 106 cells/25 cm2 flask. Incubation: at 37°C with 5% CO2. Doubling time: ca. 4 days.,,,,Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures,ACC 311,,Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ,,,,2016-08-24,2017-05-02
51117-1,51117,HBL-100,,,"CLO_0003626","CLS Cell Lines Service 300178",Homo sapiens,breast,,epithelial,,female,27,Caucasian,,not diseased,,"From CLS: The epithelial cell line HBL-100 has been derived by E.V. Gaffney and associates from the milk of a nursing mother and obtained 3 days after delivery. Although there was no evidence of a breast lesion in the milk donor, and the patient had no family history of breast cancer, the karyotype of the recovered cells was abnormal as early as passage 7. This line was able to synthesize a small amount of lactose and would respond to prolactin or estrogen by producing increased amounts of casein. Electron micrographs revealed microvilli, tonofibrils and desmosomes.",none,,,"DNA Profile (STR, source: CLS): Amelogenin: X,Y CSF1PO: 10 D13S317: 12 D16S539: 9,12 D5S818: 11,12 D7S820: 8,12 THO1: 6,8 TPOX: 8 vWA: 16 D3S1358: 14,16 D21S11: 28,30 D18S51: 16 Penta E: 7 Penta D: 12 D8S1179: 12,15 FGA: 25","adherent; From CLS: monolayer, adherent","From CLS: Medium: DMEM supplemented with 4.5 g/L glucose, L-glutamine, and 10% fetal bovine serum. Subculture: Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask); the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. Split Ratio: A ratio of 1:2 is recommended. Fluid Renewal: 2 to 3 times weekly.",,,,UCSF Cell Culture Facility,,,Obtained by Joe Gray's group (Oregon Health & Science University) from UCSF CCF,,,,2016-08-24,2016-08-24
51118-1,51118,JIMT-1,,,,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 589",Homo sapiens,breast,,epithelial,,female,62,,,"DOID:3005, invasive ductal carcinoma",breast ductal adenocarcinoma,"From DSMZ: established from the pleural effusion of a 62-year-old woman with ductal breast cancer (grade 3 invasive, T2N1M0) after postoperative radiation in 2003; cell line was described to be insensitive to HER-2-inhibiting drugs, e.g. trastuzumab (Herceptin)",none,HER-2 amplification,"Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 589",,adherent; From DSMZ: epithelial-like cells growing in monolayers,From DSMZ: Medium: 90% Dulbecco's MEM + 10% h.i. FBS. Subculture: Split confluent culture 1:2 to 1:8 every 2-4 days using trypsin/EDTA. Seed out at ca. 1 x 106 cells/80 cm2. Incubation: at 37°C with 5% CO2. Doubling time: ca. 30-40 hours.,"PMID 15634652",,,Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures,ACC 589,,Obtained by Joe Gray's group (Oregon Health & Science University) from DSMZ,,,,2016-08-24,2016-08-24
51119-1,51119,HMT-3522 S1,,,,"European Collection of Authenticated Cell Cultures (ECACC) 98102210",Homo sapiens,breast,,epithelial,,female,48,Caucasian,,"DOID:0060082, breast benign neoplasm",,"From ECACC: HMT-3522 S1, also known as HMT-3522/wt, is a subline that has been derived from HMT-3522. The parent line was established from a benign breast tumour of a 48 year old woman and has undergone spontaneous malignant transformation. It was grown on collagen in serum-free media without antibiotics from explantation onwards. HMT-3522 S1 is a continuation of this line from passage 34 growing without collagen.",none,mutation at codon 179 of P53,"European Collection of Authenticated Cell Cultures (ECACC) 98102210",,adherent,From ECACC: DMEM / Ham's F12 (1:1) + 2 mM Glutamine + 250 ng/ml insulin + 10 µg/ml transferrin +10-8 M sodium selenite + 10-10 M 17 beta-estradiol + 0.5 µg/ml hydrocortisone + 5 µg/ml ovine prolactin + 10 ng/ml EGF,"PMID 3558253",,,"Mina Bissell (Lawrence Berkeley National Laboratory, University of California, Berkeley)",,,Obtained by Joe Gray's group (Oregon Health & Science University) from Mina Bissell,,,,2016-08-24,2016-08-24
51120-1,51120,SUM102PT,SUM-102PT,,,"Asterand SUM-102PT",Homo sapiens,breast,,,,,,,,"DOID:3005, invasive ductal carcinoma",breast ductal adenocarcinoma,"From Asterand: developed from a patient with minimally invasive, ER negative and PR negative human breast carcinoma.",none,,,,adherent,"From Asterand: Ham’s F-12 + 1 g/L bovine serum albumin (fatty acid-free), 10 ng/mL EGF, 5 mM ethanolamine, 10 mM HEPES, 1 ug/mL hydrocortisone, 5 ug/mL insulin, 50 nM sodium selenite, 5 ug/mL apo-transferrin, 10 nM triiodo-L-thyronine","PMID 10604729",,,Stephen Ethier (Wayne State University and University of Michigan Medical School),,,Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier,,,,2016-08-24,2016-08-24
51121-1,51121,SUM190PT,SUM-190PT,,,"Asterand SUM-190PT",Homo sapiens,breast,,,,,,,,"DOID:6263, inflammatory breast carcinoma",inflammatory luminal breast carcinoma,From Asterand: derived from a primary human inflammatory luminal breast carcinoma,none,,,,adherent,"From Asterand: Ham’s F-12 + 1 g/L bovine serum albumin, 5 mM ethanolamine, 10 mM HEPES, 1 ug/mL hydrocortisone, 5 ug/mL insulin, 50 nM sodium selenite, 5 ug/mL apo-transferrin, 10 nM triiodo-L-thyronine","PMID 10604729",,,Stephen Ethier (Wayne State University and University of Michigan Medical School),,,Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier,,,,2016-08-24,2016-08-24
51122-1,51122,SUM44PE,SUM-44PE,,,"Asterand SUM-44PE",Homo sapiens,breast,,,,,,,,"DOID:3459, breast carcinoma",luminal breast carcinoma,From Asterand: derived from primary human luminal breast epithelial cells of a pleural effusion.,none,,,,adherent,"From Asterand: Ham’s F-12 + 1 g/L bovine serum albumin, 5 mM ethanolamine, 10 mM HEPES, 1 ug/mL hydrocortisone, 5 ug/mL insulin, 50 nM sodium selenite, 5 ug/mL apo-transferrin, 10 nM triiodo-L-thyronine","PMID 10604729",,,Stephen Ethier (Wayne State University and University of Michigan Medical School),,,Obtained by Joe Gray's group (Oregon Health & Science University) from Stephen Ethier,,,,2016-08-24,2016-08-24
51123-1,51123,HMT-3522 T4-2,,,,"European Collection of Authenticated Cell Cultures (ECACC) 98102212",Homo sapiens,breast,,epithelial,,female,48,Caucasian,,"DOID:0060082, breast benign neoplasm",,"From ECACC: HMT-3522 T4-2, also known as HMT-3522/mt-1, is a subline that has been derived from HMT-3522. The parent line was established from a benign breast tumour of a 48 year old woman and has undergone spontaneous malignant transformation. It was grown on collagen in serum-free media without antibiotics from explantation onwards. A mutation at codon 179 of the p53 gene was detected during establishment of the parent line at passage 25-30. The subline S1 is a continuation of this line from passage 34 (ECACC cataogue no. 98102210) growing without collagen. Subline S2 (ECACC catalogue no. 98102211) has been isolated by culturing S1 cells from passage 118 in serum-free media without epidermal growth factor (EGF). The third subline, T4-2 was established from S2 at passage 238 and is the only tumorigenic line in nude mice out of the 3 sublines.",none,mutation at codon 179 of P53; trisomy for the short arm of chromosome 7,"European Collection of Authenticated Cell Cultures (ECACC) 98102212","DNA Profile (STR, source: Asterand): STR-PCR Data: Amelogenin: X CSF1PO: 11,12 D13S317: 11 D16S539: 11,13 D5S818: 9,11 D7S 820: 8,9 THO1: 9,9.3 TPOX: 8,11 vWA: 14,17",adherent,From ECACC: DMEM / Ham's F12 (1:1) + 2 mM Glutamine + 250 ng/ml insulin + 10 µg / ml transferrin +10-8 M Sodium selenite + 10-10 M 17 beta-estradiol + 0.5 µg/ml hydrocortisone + 5 µg / ml ovine prolactin,"PMID 3558253",,,"Mina Bissell (Lawrence Berkeley National Laboratory, University of California, Berkeley)",,,Obtained by Joe Gray's group (Oregon Health & Science University) from Mina Bissell,,,,2016-08-24,2016-08-24