Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with EGF or four kinase inhibitors - Dataset (ID:20267)

Antibodies Studied

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  • 1 to 25 of 28 Antibodies
HMS LINCS ID Name Alternative Names Clone Name LINCS ID Resource Identification Initiative ID Nominal Protein Target HMS LINCS ID(s)
80001-1
H3 (pS10)
Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #3475
D2C8 AB_10694639
80002-2
p21 / Alexa Fluor 647
p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 647 Conjugate) #8587
12D1 AB_10892861
80003-1
KI-67 / clone D3B5
Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) #11882
D3B5
80004-1
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555
Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #8957
D20B12
80005-1
S6 (pS235; pS236) / Alexa Fluor 647
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #4851
D57.2.2E AB_916160
80006-1
S6 (pS240; pS244) / Alexa Fluor 488
Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP®Rabbit mAb (Alexa Fluor® 488 Conjugate) #5018
D68F8 AB_10695861
80007-1
rabbit IgG(H+L) / goat / cross-adsorbed
Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate
AB_143165
80008-1
EGFR
EGF Receptor (D38B1) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #5616
D38B1 AB_10691853
80011-1
PCNA
PCNA (PC10) Mouse mAb (Alexa Fluor® 488 Conjugate) #8580
PC10 AB_11178664
80012-1
ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) / Alexa Fluor 488
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #4344
D13.14.4E AB_2139960
80013-1
Keratin
Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 555 Conjugate) #3478
C11 AB_10829040
K4; K5; K6A; K6B; K6C; K8; K10; K13; K18
80016-1
Beta-actin
β-Actin (13E5) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8046
13E5 AB_11179208
80022-1
p53
p53 Antibody (DO-1): sc-126
DO-1 AB_628082
80024-1
mouse IgG(H+L) / goat
Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 647 conjugate
AB_10564125
80025-1
Aurora kinase A (pT288); Aurora kinase B (pT232); Aurora kinase C (pT198)
Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #13464
D13A11
80026-1
S6
S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 555 Conjugate) #6989
54D2 AB_10828226
80027-1
VEGFR-2
VEGF Receptor 2 (D5B1) Rabbit mAb (Alexa Fluor® 555 Conjugate) #12872
D5B1
80028-1
PARP-1 (D214-cleaved)
Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #6894
D64E10 AB_10830735
80029-1
p27Kip1 / clone Y236
Anti-p27 KIP 1 antibody [Y236] (Alexa Fluor® 647) (ab194234)
Y236
80030-1
H2A.X (pS139) / clone 20E3 / Alexa Fluor 647
Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor® 647 Conjugate) #9720
20E3 AB_10692910
80031-1
AKT
Akt (pan) (C67E7) Rabbit mAb (Alexa Fluor® 647 Conjugate) #5186
C67E7 AB_10695877
80032-1
mTOR
mTOR (7C10) Rabbit mAb (Alexa Fluor® 647 Conjugate) #5048
7C10 AB_10828101
80033-1
Vimentin
Vimentin (D21H3) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #9856
D21H3 AB_10834530
80034-1
NF-kB p65
Anti-NF-kB p65 antibody [E379] (Alexa Fluor® 647) (ab190589)
E379
80035-1
FOXO3a
FoxO3a (75D8) Rabbit mAb #2497
75D8 AB_836876
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  • 1 to 25 of 28 Antibodies