HMS LINCS Batch ID,HMS LINCS ID,Name,Alternative Names,LINCS ID,Alternative ID,Reference Source,Organism,Organ,Tissue,Cell Type,Details of Cell Type,Donor Sex,Donor Age,Donor Ethnicity,Donor Health Status,Disease,Details of Disease,Production Details,Genetic Modification(s),Known Mutations,Citation Information for Mutations,Verification Reference Profile,Growth Properties,Recommended Culture Conditions,Relevant Citations,Usage Note,Comments,Provider,Provider Catalog ID,Provider Batch ID,Source Information,Date Received,HMS QC Outcome,Transient Modification(s),Date Publicly Available,Most Recent Update
50029-3,50029,MCF7,,LCL-1460,"CLO_0007606","ATCC HTB-22",Homo sapiens,breast,,epithelial,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS905946","DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15",adherent,"From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1 mM sodium pyruvate and supplemented with 0.01 mg/ml bovine insulin, 90%; fetal bovine serum, 10%.
Protocol: Remove culture medium to a centrifuge tube.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Transfer the cell suspension to the centrifuge tube with the medium and cells from step 1, and centrifuge at approximately 125 x g for 5 to 10 minutes. Discard the supernatant.
Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended",,,,ATCC,HTB-22,,"Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010.",2010-07-16,PASS,,2012-04-25,2016-04-04
50057-3,50057,SK-BR-3,SKBr3,LCL-1475,"CLO_0009034","ATCC HTB-30",Homo sapiens,breast,,epithelial,,female,,,,"DOID:3459, breast carcinoma",,,none,,,"DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 12
D13S317: 11,12
D16S539: 9
D5S818: 9,12
D7S820: 9,12
THO1: 8,9
TPOX: 8,11
vWA: 17",adherent,"ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0 deg C
Subculturing protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin, 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37 deg C.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week",,,,ATCC,HTB-30,,"Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010",2010-07-16,PASS,,2012-05-01,2016-07-12
50058-4,50058,MDA-MB-231,MDAMB231; MDA-MB231,LCL-1461,"CLO_0007634","ATCC HTB-26",Homo sapiens,breast,,epithelial,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS905960","DNA Profile (STR, source: ATCC):
Amelogenin: X
CSF1PO: 12,13
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 8,9
THO1: 7,9.3
TPOX: 8,9
vWA: 15,18",adherent,"ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 37.0 deg C
Subculturing protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37 deg C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37 deg C without CO2.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week",,,,ATCC,HTB-26,,"Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010",2010-07-16,PASS,,2012-05-01,2016-07-12
50105-3,50105,BT-20,,LCL-1476,"CLO_0002041","ATCC HTB-19",Homo sapiens,breast,,,,female,,,,"DOID:3459, breast carcinoma",breast ductal carcinoma,,none,,"COSS906801",,adherent,"From MGH/CMT as specified by cell provider: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended",,,,ATCC,HTB-19,,"Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010",2010-07-16,PASS,,2012-07-31,2016-04-04
50238-3,50238,Hs 578T,Hs-578-T,LCL-1315,"CLO_0004009","ATCC HTB-126",Homo sapiens,breast,,,,female,,,,"DOID:3008, ductal carcinoma",breast ductal carcinoma,,none,,"COSS905957",,adherent,"From MGH/CMT as specified by cell provider: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.01 mg/ml bovine insulin and 10% fetal bovine serum.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended",,,,ATCC,HTB-126,,"Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010",2010-07-16,PASS,,2012-07-31,2016-07-12
50583-7,50583,MCF 10A,,LCL-2085,"CLO_0007599","ATCC CRL-10317",Homo sapiens,breast,,epithelial,,female,,,,fibrocystic disease,non-tumorigenic,,none,,,,adherent,,,,,ATCC,CRL-10317,,"Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010",2010-07-16,PASS,,2012-11-02,2016-04-04