Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

Data Columns

  • 23 Data Columns
Name Description Display Name Data Type Unit Precision Readout Type Replicate Comments
primaryAntibody
Primary antibody reagent
 Primary Antibody antibody
secondaryAntibody
Secondary antibody reagent
 Secondary Antibody antibody
cell
Cell reagent
 Cell cell
yCoordinate
Y-coordinate of the region of interest in the image field
 Y-coordinate Numeric 2
xCoordinate
X-coordinate of the region of interest in the image field
 X-coordinate Numeric 2
field Field Numeric 0
measuredfluorescenceintensityAU Measured Fluorescence Intensity (a.u.) Numeric 1
cellCircularity Cell Circularity Numeric 3
cellperimeterUm Cell Perimeter (um) Numeric 2
cellareaUm2 Cell Area (um^2) Numeric 0
timepoint Timepoint Numeric 0
registeredImage
Link to composite image file with data from both CycIF cycles
 Registered Image omero_image
rawImage
Link to raw image file from the designated cycle of CycIF
 Raw Image omero_image
otherReagentDetected
Other reagent detected by fluorescence
 Other Reagent Detected other_reagent
proteinDetected
Protein detected by immunofluorescence with the specified antibodies
 Protein Detected protein
smallMolecule
Small molecule perturbagen
 Small Molecule small_molecule
smallMolConcUnit Small Mol Conc Unit Text
smallMolConcentration Small Mol Concentration Text
timepointUnit Timepoint Unit Text
well Well Text
cycIFRound CycIF Round Text
cytellfilter Cytell filter Text
cellIDNumber Cell ID Number Text
  • 23 Data Columns