Highly multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the COLO 858 melanoma cell line - Dataset (ID:20236)

Antibodies Studied

  • 7 Antibodies
HMS LINCS ID Name Alternative Names Clone Name LINCS ID Resource Identification Initiative ID Nominal Protein Target HMS LINCS ID(s)
80001
H3 (pS10)
Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #3475
D2C8 AB_10694639
80002
p21 / Alexa Fluor 647
p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 647 Conjugate) #8587
12D1 AB_10892861
80003
KI-67 / clone D3B5
Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) #11882
D3B5
80004
Rb (pS807; pS811); Rb (pS807); Rb (pS811) / clone D20B12 / Alexa Fluor 555
Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #8957
D20B12
80005
S6 (pS235; pS236) / Alexa Fluor 647
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #4851
D57.2.2E AB_916160
80006
S6 (pS240; pS244) / Alexa Fluor 488
Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP®Rabbit mAb (Alexa Fluor® 488 Conjugate) #5018
D68F8 AB_10695861
80007
rabbit IgG(H+L) / goat / cross-adsorbed
Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate
AB_143165
  • 7 Antibodies