Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | MCF 10A | 10.0 | uM | 24 | hr | 144.97 | 21.29 | p-Akt | 18.43 | |
| Dactolisib | T47D | 10.0 | uM | 6 | hr | 85.23 | 24.35 | p-4EBP1 | 5.42 | |
| Triciribine | T47D | 3.16 | uM | 6 | hr | 103.46 | 24.67 | p-Akt | 8.36 | |
| Gefitinib | MCF 10A | 3.16 | uM | 24 | hr | 173.69 | 24.68 | p-Akt | 20.53 | |
| Torkinib | HCC1954 | 2.37 | uM | 6 | hr | 143.76 | 24.81 | p-S6 | 9.70 | |
| Torkinib | HCC1954 | 562.0 | nM | 6 | hr | 146.89 | 24.95 | p-S6 | 10.19 | |
| Torkinib | HCC1954 | 10.0 | uM | 6 | hr | 173.43 | 25.17 | p-S6 | 9.62 | |
| Torkinib | HCC1954 | 133.0 | nM | 6 | hr | 159.90 | 25.42 | p-S6 | 10.66 | |
| Triciribine | T47D | 3.16 | uM | 6 | hr | 95.94 | 25.51 | p-4EBP1 | 82.61 | |
| Torkinib | HCC1954 | 562.0 | nM | 6 | hr | 166.11 | 26.07 | p-Akt | 12.65 | |
| Triciribine | T47D | 1.0 | uM | 6 | hr | 101.24 | 26.12 | p-Akt | 9.22 | |
| Gefitinib | MCF 10A | 3.16 | uM | 24 | hr | 178.17 | 26.30 | p-4EBP1 | 151.62 | |
| Torkinib | HCC1954 | 2.37 | uM | 6 | hr | 174.43 | 26.36 | p-Akt | 12.16 | |
| Gefitinib | MCF 10A | 10.0 | uM | 24 | hr | 162.20 | 26.49 | p-4EBP1 | 179.07 | |
| Gefitinib | MCF 10A | 10.0 | uM | 24 | hr | 178.43 | 26.75 | p-4EBP1 | 176.62 | |
| Dactolisib | T47D | 0 (control) | 6 | hr | 99.23 | 26.75 | p-Akt | 14.05 | | |
| Torkinib | HCC1954 | 2.37 | uM | 6 | hr | 169.96 | 27.00 | p-S6 | 11.34 | |
| Torkinib | HCC1954 | 31.6 | nM | 6 | hr | 144.70 | 27.42 | p-4EBP1 | 31.74 | |
| Gefitinib | MCF 10A | 3.16 | uM | 24 | hr | 206.94 | 27.53 | p-Akt | 24.17 | |
| Gefitinib | MCF 10A | 10.0 | uM | 24 | hr | 173.33 | 27.59 | p-S6 | 20.83 | |
| Dactolisib | T47D | 100.0 | nM | 6 | hr | 75.87 | 27.61 | p-4EBP1 | 41.17 | |
| Gefitinib | MCF 10A | 10.0 | uM | 24 | hr | 187.17 | 27.83 | p-S6 | 16.12 | |
| Dactolisib | T47D | 1.0 | nM | 6 | hr | 92.26 | 27.93 | p-4EBP1 | 75.18 | |
| Torkinib | HCC1954 | 10.0 | uM | 6 | hr | 163.54 | 28.07 | p-4EBP1 | 11.75 | |
| Gefitinib | MCF 10A | 3.16 | uM | 24 | hr | 178.06 | 28.30 | p-4EBP1 | 142.46 | |