Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | AU565 | 0 (control) | 6 | hr | 286.03 | 62.73 | p-Akt | 29.07 | | |
| Gefitinib | AU565 | 316.0 | nM | 6 | hr | 228.71 | 57.03 | p-Akt | 21.72 | |
| Gefitinib | AU565 | 316.0 | nM | 6 | hr | 289.30 | 66.48 | p-4EBP1 | 93.41 | |
| Gefitinib | AU565 | 316.0 | nM | 6 | hr | 234.01 | 54.85 | p-Akt | 22.11 | |
| Gefitinib | AU565 | 10.0 | nM | 6 | hr | 241.48 | 51.68 | p-Akt | 25.73 | |
| Gefitinib | AU565 | 1.0 | nM | 6 | hr | 289.01 | 63.74 | p-4EBP1 | 68.42 | |
| Gefitinib | AU565 | 31.6 | nM | 6 | hr | 275.23 | 61.32 | p-4EBP1 | 73.02 | |
| Gefitinib | AU565 | 31.6 | nM | 6 | hr | 224.67 | 51.67 | p-Akt | 24.54 | |
| Gefitinib | AU565 | 100.0 | nM | 6 | hr | 256.41 | 58.17 | p-Akt | 25.26 | |
| Gefitinib | AU565 | 3.16 | nM | 6 | hr | 226.81 | 47.26 | p-4EBP1 | 50.71 | |
| Gefitinib | AU565 | 10.0 | nM | 6 | hr | 257.60 | 59.37 | p-S6 | 139.49 | |
| Gefitinib | AU565 | 3.16 | uM | 6 | hr | 194.79 | 43.73 | p-S6 | 15.66 | |
| Gefitinib | AU565 | 3.16 | nM | 6 | hr | 268.55 | 59.48 | p-S6 | 131.60 | |
| Gefitinib | AU565 | 3.16 | uM | 6 | hr | 207.63 | 45.48 | p-Akt | 16.31 | |
| Gefitinib | AU565 | 3.16 | nM | 6 | hr | 250.47 | 55.69 | p-S6 | 122.30 | |
| Gefitinib | AU565 | 100.0 | nM | 6 | hr | 277.70 | 67.19 | p-4EBP1 | 80.51 | |
| Gefitinib | AU565 | 100.0 | nM | 6 | hr | 246.22 | 60.31 | p-S6 | 101.43 | |
| Gefitinib | AU565 | 1.0 | uM | 6 | hr | 218.20 | 49.17 | p-S6 | 35.46 | |
| Gefitinib | AU565 | 1.0 | uM | 6 | hr | 215.97 | 50.77 | p-S6 | 32.49 | |
| Gefitinib | AU565 | 3.16 | uM | 6 | hr | 233.77 | 53.87 | p-4EBP1 | 66.06 | |
| Gefitinib | AU565 | 1.0 | nM | 6 | hr | 244.78 | 52.81 | p-4EBP1 | 55.56 | |
| Gefitinib | AU565 | 31.6 | nM | 6 | hr | 286.23 | 60.88 | p-Akt | 29.80 | |
| Gefitinib | AU565 | 31.6 | nM | 6 | hr | 286.70 | 67.72 | p-S6 | 146.86 | |
| Gefitinib | AU565 | 0 (control) | 6 | hr | 234.75 | 54.33 | p-Akt | 24.82 | | |
| Gefitinib | AU565 | 3.16 | nM | 6 | hr | 235.59 | 52.34 | p-4EBP1 | 50.68 | |