HMS LINCS Batch ID,HMS LINCS ID,Name,Alternative Names,LINCS ID,Alternative ID,Reference Source,Organism,Organ,Tissue,Cell Type,Details of Cell Type,Donor Sex,Donor Age,Donor Ethnicity,Donor Health Status,Disease,Details of Disease,Production Details,Genetic Modification(s),Known Mutations,Citation Information for Mutations,Verification Reference Profile,Growth Properties,Recommended Culture Conditions,Relevant Citations,Usage Note,Comments,Provider,Provider Catalog ID,Provider Batch ID,Source Information,Date Received,HMS QC Outcome,Transient Modification(s),Date Publicly Available,Most Recent Update
50091-2,50091,AU565,AU-565,LCL-1462,"CLO_0001769","ATCC CRL-2351",Homo sapiens,breast,,,,female,,,,"DOID:3458, adenocarcinoma",breast adenocarcinoma,,none,,"COSS910704",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Subculturing: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended",,,,Mario Niepel (Harvard Medical School),,,"Obtained by Mario Niepel (Harvard Medical School) from ATCC as part of the ICBP43 Collection in July, 2010, and distributed to Mohammad Fallahi-Sichani in December of 2012.",2012-12,,,2012-07-31,2016-04-04
50213-2,50213,HCC1954,HCC-1954,LCL-1332,"CLO_0003647","ATCC CRL-2338",Homo sapiens,breast,,,,female,,,,"DOID:3008, primary ductal carcinoma",breast ductal carcinoma,,none,,"COSS749709",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:8 is recommended",,,,Mario Niepel (Harvard Medical School),,,"Obtained by Mario Niepel (Harvard Medical School) from ATCC as part of the ICBP43 Collection in July, 2010, and distributed to Mohammad Fallahi-Sichani in December of 2012.",2012-12,,,2012-07-31,2016-04-04
50541-2,50541,T47D,T-47D,LCL-1486,"CLO_0009251","ATCC HTB-133",Homo sapiens,breast,,,,female,,,,"DOID:3459, breast carcinoma",,,none,,"COSS905945",,adherent,"From MGH/CMT as specified by cell provider: RPMI 1640 or DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 37C.",,,,Mario Niepel (Harvard Medical School),,,"Obtained by Mario Niepel (Harvard Medical School) from ATCC as part of the ICBP43 Collection in July, 2010, and distributed to Mohammad Fallahi-Sichani in December of 2012.",2012-12,,,2012-07-31,2016-07-12
50583-5,50583,MCF 10A,,LCL-2085,"CLO_0007599","ATCC CRL-10317",Homo sapiens,breast,,epithelial,,female,,,,fibrocystic disease,non-tumorigenic,,none,,,,adherent,,,,,Mario Niepel (Harvard Medical School),,,"Obtained by Mario Niepel (Harvard Medical School) from ATCC as part of the ICBP43 Collection in July, 2010, and distributed to Mohammad Fallahi-Sichani in December of 2012.",2012-12,,,2012-11-02,2016-04-04