| HMS LINCS ID:
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51124
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| Name:
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MCF 10A-H2B-mCherry
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| Alternative Names:
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| LINCS ID:
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| Alternative ID:
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CLO_0007599
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| Parent Cell Line:
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HMSL50583-7: MCF 10A
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| Reference Source:
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Mario Niepel (Harvard Medical School)
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| Organism:
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Homo sapiens
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| Organ:
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breast
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| Tissue:
|
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| Cell Type:
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epithelial
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| Details of Cell Type:
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| Donor Sex:
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female
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| Donor Age:
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| Donor Ethnicity:
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| Donor Health Status:
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| Disease:
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fibrocystic disease
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| Details of Disease:
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non-tumorigenic
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| Production Details:
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Modification type: retroviral transformation with an H2B-mCherry expression cassette that comprised AAVS1 homology arms, the hPGK promotor, and SV40 polyA terminator and integration into the AAVS1 safe harbor genomic locus using CRISPR/Cas9; followed by clonal selection based on transgene expression level. Citations: H2B-mCherry expression cassette: gift of Robert Benezra (Memorial Sloan Kettering Cancer Center), Addgene plasmid #20972; SV40 polyA terminator: gift from Rudolf Jaenisch (Whitehead Institute for Biomedical Research), Addgene plasmid # 22072.
|
| Genetic Modification(s):
|
H2B-mCherry integration at AAVS1 safe harbor locus
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| Known Mutations:
|
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| Citation Information for Mutations:
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| Verification Reference Profile:
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| Growth Properties:
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adherent
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| Recommended Culture Conditions:
|
|
| Relevant Citations:
|
PMID 27135972
|
| Usage Note:
|
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| Comments:
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| Date Publicly Available:
|
2016-08-24
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| Most Recent Update:
|
2016-08-24
|