UACC-812 - Cell Line (ID:50556)

HMS LINCS ID: 50556
Name: UACC-812
Alternative Names:
LINCS ID: LCL-1319
Alternative ID: CLO_0009467
Parent Cell Line:
Reference Source: ATCC CRL-1897
Organism: Homo sapiens
Organ: breast
Tissue:
Cell Type:
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3008, ductal carcinoma
Details of Disease: breast ductal carcinoma
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS910910
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with 20 ng/ml human EGF and 20% fetal bovine serum. Growth Conditions: The cells grow very slowly, and growth is enhanced by using 20% fetal bovine serum and adding epidermal growth factor (20 ng/ml) to the medium. Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Relevant Citations:
Usage Note:
Comments:
Date Publicly Available: 2012-07-31
Most Recent Update: 2016-04-04

Datasets:

HMS Dataset ID Dataset Title HMS Dataset Type
20120
Metrics other than potency reveal systematic variation in responses to cancer drugs
Analysis
20137
Basal profile of receptor tyrosine kinase signaling network measured by ELISA
ELISA
20138
Cell signaling response to growth factors measured by high throughput microscopy
Microscopy/Imaging
20139
Cell signaling response to cytokines measured by high throughput microscopy
Microscopy/Imaging
20140
Cell signaling response to growth factors measured by ELISA
ELISA
20268
Growth rate-corrected (GR) dose-response metrics across a panel of 71 breast cancer cell lines treated with a library of small molecule and antibody perturbagens. Dataset 1 of 4: Relative cell counts and normalized growth rate inhibition values across technical replicates.
Analysis
20269
Growth rate-corrected (GR) dose-response metrics across a panel of 71 breast cancer cell lines treated with a library of small molecule and antibody perturbagens. Dataset 2 of 4: Calculated dose-response metrics.
Analysis

Batch Information:

HMS LINCS Batch ID Provider Provider Catalog ID Provider Batch ID
50556-1 ATCC CRL-1897
50556-2 ATCC CRL-1897