UACC-812 - Cell Line (ID:50556)

HMS LINCS ID: 50556
Name: UACC-812
Alternative Names:
LINCS ID: LCL-1319
Alternative ID: CLO_0009467
Parent Cell Line:
Reference Source: ATCC CRL-1897
Organism: Homo sapiens
Organ: breast
Tissue:
Cell Type:
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3008, ductal carcinoma
Details of Disease: breast ductal carcinoma
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS910910
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with 20 ng/ml human EGF and 20% fetal bovine serum. Growth Conditions: The cells grow very slowly, and growth is enhanced by using 20% fetal bovine serum and adding epidermal growth factor (20 ng/ml) to the medium. Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Relevant Citations:
Usage Note:
Comments:
Date Publicly Available: 2012-07-31
Most Recent Update: 2016-04-04

Batch Information for HMSL50556-2:

HMS LINCS Batch ID: 50556-2
Provider: ATCC
Provider Catalog ID: CRL-1897
Provider Batch ID:
Source Information: Obtained by Mario Niepel (Harvard Medical School) as part of the ICBP43 Collection in July, 2010
Date Received: 2010-07-16
HMS QC Outcome:
Transient Modification(s):
Comments:
Date Publicly Available: 2016-06-06
Most Recent Update: 2016-07-12