MDA-MB-157 - Cell Line (ID:50328)

HMS LINCS ID: 50328
Name: MDA-MB-157
Alternative Names:
LINCS ID: LCL-1916
Alternative ID: CLO_0007632
Parent Cell Line:
Reference Source: ATCC HTB-24
Organism: Homo sapiens
Organ: breast
Tissue:
Cell Type:
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:5605, breast medullary carcinoma
Details of Disease:
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS925338
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: LLeibovitz's L-15 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%. Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C without CO2. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Relevant Citations:
Usage Note:
Comments:
Date Publicly Available: 2012-07-31
Most Recent Update: 2016-04-04

Datasets:

HMS Dataset ID Dataset Title HMS Dataset Type
20120
Metrics other than potency reveal systematic variation in responses to cancer drugs
Analysis
20137
Basal profile of receptor tyrosine kinase signaling network measured by ELISA
ELISA
20138
Cell signaling response to growth factors measured by high throughput microscopy
Microscopy/Imaging
20139
Cell signaling response to cytokines measured by high throughput microscopy
Microscopy/Imaging
20140
Cell signaling response to growth factors measured by ELISA
ELISA
20268
Growth rate-corrected (GR) dose-response metrics across a panel of 71 breast cancer cell lines treated with a library of small molecule and antibody perturbagens. Dataset 1 of 4: Relative cell counts and normalized growth rate inhibition values across technical replicates.
Analysis
20269
Growth rate-corrected (GR) dose-response metrics across a panel of 71 breast cancer cell lines treated with a library of small molecule and antibody perturbagens. Dataset 2 of 4: Calculated dose-response metrics.
Analysis
20343
Breast Cancer Profiling Project, Drug Sensitivity 1: Fixed-cell GR measures of 35 breast cell lines to 34 small molecule perturbagens. Dataset 1 of 2: Normalized growth rate inhibition values.
Microscopy/Imaging
20344
Breast Cancer Profiling Project, Drug Sensitivity 1: Fixed-cell GR measures of 35 breast cell lines to 34 small molecule perturbagens. Dataset 2 of 2: Calculated dose response metrics.
Analysis
20348
Breast Cancer Profiling Project, Gene Expression 1: Baseline mRNA sequencing on 35 breast cell lines
RNA-Seq
20352
Breast Cancer Profiling Project – Proteomics 1: 1 total proteome dataset for a 35-cell line breast cancer panel under basal conditions
Proteomics
20353
Breast Cancer Profiling Project – Proteomics 2: 1 phosphoproteome dataset (including phosphotyrosine enrichment) for a 35-cell line breast cancer panel under basal conditions
Proteomics

Batch Information:

HMS LINCS Batch ID Provider Provider Catalog ID Provider Batch ID
50328-1 ATCC HTB-24
50328-2 ATCC HTB-24