HCC202 - Cell Line (ID:50214)
|HMS LINCS ID:||50214|
|Parent Cell Line:|
|Reference Source:||ATCC CRL-2316|
|Details of Cell Type:|
|Donor Health Status:|
|Disease:||DOID:3008, primary ductal carcinoma|
|Details of Disease:||breast ductal carcinoma|
|Citation Information for Mutations:||COSS1290906|
|Verification Reference Profile:|
|Recommended Culture Conditions:||From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Subculturing: Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended|
|Date Publicly Available:||2012-07-31|
|Most Recent Update:||2016-04-04|
|HMS Dataset ID||Dataset Title||HMS Dataset Type|
Metrics other than potency reveal systematic variation in responses to cancer drugs
Basal profile of receptor tyrosine kinase signaling network measured by ELISA
Cell signaling response to growth factors measured by high throughput microscopy
Cell signaling response to cytokines measured by high throughput microscopy
Cell signaling response to growth factors measured by ELISA
Growth rate-corrected (GR) dose-response metrics across a panel of 71 breast cancer cell lines treated with a library of small molecule and antibody perturbagens. Dataset 1 of 4: Relative cell counts and normalized growth rate inhibition values across technical replicates.
Growth rate-corrected (GR) dose-response metrics across a panel of 71 breast cancer cell lines treated with a library of small molecule and antibody perturbagens. Dataset 2 of 4: Calculated dose-response metrics.
Breast Cancer Deubiquitinating Enzyme (DUB) Inhibitor Profiling: Fixed-cell GR measures of 21 breast cell lines to 32 small molecule perturbagens. Dataset 1 of 2: Normalized growth rate inhibition values.
Breast Cancer Deubiquitinating Enzyme (DUB) Inhibitor Profiling: Fixed-cell GR measures of 21 breast cell lines to 32 small molecule perturbagens. Dataset 2 of 2: Calculated dose response metrics.
|HMS LINCS Batch ID||Provider||Provider Catalog ID||Provider Batch ID|