HCC1806 - Cell Line (ID:50211)

HMS LINCS ID: 50211
Name: HCC1806
Alternative Names: HCC-1806
LINCS ID: LCL-1960
Alternative ID: CLO_0003644
Parent Cell Line:
Reference Source: ATCC CRL-2335
Organism: Homo sapiens
Organ: breast
Tissue:
Cell Type:
Details of Cell Type:
Donor Sex: female
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:7459, primary acantholytic squamous cell carcino
Details of Disease: breast ductal carcinoma
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS907047
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Subculturing: Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Relevant Citations:
Usage Note:
Comments:
Date Publicly Available: 2012-07-31
Most Recent Update: 2016-04-04

Batch Information for HMSL50211-1:

HMS LINCS Batch ID: 50211-1
Provider: ATCC
Provider Catalog ID: CRL-2335
Provider Batch ID:
Source Information: Obtained by Joe Gray's group (Oregon Health & Science University) from ATCC
Date Received:
HMS QC Outcome:
Transient Modification(s):
Comments:
Date Publicly Available: 2012-07-31
Most Recent Update: 2015-06-29