KYSE-140 - Cell Line (ID:50024)

HMS LINCS ID: 50024
Name: KYSE-140
Alternative Names:
LINCS ID: LCL-1547
Alternative ID: CLO_0007128
Parent Cell Line:
Reference Source: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 348
Organism: Homo sapiens
Organ: esophagus
Tissue:
Cell Type:
Details of Cell Type:
Donor Sex: male
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:3748, esophageal squamous cell carcinoma
Details of Disease:
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS753573
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: 90% RPMI 1640 + 10% FBS split confluent culture 1:5 to 1:10 every 2-4 days using trypsin/EDTA (cells may need five or more minutes to detach from the flask; prior to using trypsin, cells should be washed twice with PBS); seed out at ca. 2-3 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of ca. 20-30 x 10 6 cells/175 cm 2
Relevant Citations:
Usage Note:
Date Publicly Available: 2012-04-25
Most Recent Update: 2016-08-24

Datasets:

HMS Dataset ID Dataset Title HMS Dataset Type
20002
Moerke 3 Color Apoptosis
Microscopy/Imaging
20003
Tang Mitosis/Apoptosis ver. II
Microscopy/Imaging
20004
Tang Proliferation/Mitosis
Microscopy/Imaging

Batch Information:

HMS LINCS Batch ID Provider Provider Catalog ID Provider Batch ID
50024-1 Center for Molecular Therapeutics (CMT; Massachusetts General Hospital) MGH-496
50024-2 Center for Molecular Therapeutics (CMT; Massachusetts General Hospital) MGH-496