BPH-1 - Cell Line (ID:50005)

HMS LINCS ID: 50005
Name: BPH-1
Alternative Names:
LINCS ID: LCL-2095
Alternative ID: CLO_0002022
Parent Cell Line:
Reference Source: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures ACC 143
Organism: Homo sapiens
Organ: prostate
Tissue:
Cell Type: epithelioid
Details of Cell Type:
Donor Sex: male
Donor Age:
Donor Ethnicity:
Donor Health Status:
Disease: DOID:0060087, male reproductive benign neoplasm
Details of Disease: benign prostate hyperplasia
Production Details:
Genetic Modification(s): none
Known Mutations:
Citation Information for Mutations: COSS924105
Verification Reference Profile:
Growth Properties: adherent
Recommended Culture Conditions: From MGH/CMT as specified by cell provider: 80% RPMI 1640 + 20% FBS + 20 ng/ml testosterone + 5 microg/ml transferrin + 5 ng/ml sodium selenite + 5 microg/ml insulin + trace elements mix (a list can be provided as they are no longer commercially available in a single mix) split confluent culture 1:3 to 1:8 every 3-7 days using trypsin/EDTA (cells should be incubated for more than 10 min with trypsin/EDTA at 37C); seed out at 0.1 x 10 5 cells/cm 2 ; we noted that the cells grow equally well without the trace element mix which was recommended by the depositor at 37C with 5% CO2 cell harvest of ca. 0.4 x 105 cells/cm 2
Relevant Citations:
Usage Note:
Date Publicly Available: 2012-04-25
Most Recent Update: 2016-08-24

Datasets:

HMS Dataset ID Dataset Title HMS Dataset Type
20002
Moerke 3 Color Apoptosis
Microscopy/Imaging
20004
Tang Proliferation/Mitosis
Microscopy/Imaging

Batch Information:

HMS LINCS Batch ID Provider Provider Catalog ID Provider Batch ID
50005-1 Center for Molecular Therapeutics (CMT; Massachusetts General Hospital) MGH-997
50005-2 Center for Molecular Therapeutics (CMT; Massachusetts General Hospital) MGH-997